Kanehisa Yokoyama
Yamaguchi University
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Publication
Featured researches published by Kanehisa Yokoyama.
Nucleic Acids Research | 2006
Kenji Kinoshita; Kentaro Fujimoto; Toru Yakabe; Shin Saito; Yuzo Hamaguchi; Takayuki Kikuchi; Ken Nonaka; Shigenori Murata; Daisuke Masuda; Wataru Takada; Sohei Funaoka; Susumu Arai; Hisao Nakanishi; Kanehisa Yokoyama; Kazuhiko Fujiwara; Kenichi Matsubara
DNA microarrays are routinely used to monitor gene expression profiling and single nucleotide polymorphisms (SNPs). However, for practically useful high performance, the detection sensitivity is still not adequate, leaving low expression genes undetected. To resolve this issue, we have developed a new plastic S-BIO® PrimeSurface® with a biocompatible polymer; its surface chemistry offers an extraordinarily stable thermal property for a lack of pre-activated glass slide surface. The oligonucleotides immobilized on this substrate are robust in boiling water and show no significant loss of hybridization activity during dissociation treatment. This allowed us to hybridize the templates, extend the 3′ end of the immobilized DNA primers on the S-Bio® by DNA polymerase using deoxynucleotidyl triphosphates (dNTP) as extender units, release the templates by denaturalization and use the same templates for a second round of reactions similar to that of the PCR method. By repeating this cycle, the picomolar concentration range of the template oligonucleotide can be detected as stable signals via the incorporation of labeled dUTP into primers. This method of Multiple Primer EXtension (MPEX) could be further extended as an alternative route for producing DNA microarrays for SNP analyses via simple template preparation such as reverse transcript cDNA or restriction enzyme treatment of genome DNA.
Archive | 2006
Mitsutaka Matsumoto; Sumio Shibahara; Takayuki Matsumoto; Kanehisa Yokoyama; Sohei Funaoka; Daisuke Masuda; Michael Patrick Coleman; Dominic Zichi
Archive | 2003
Hisao Nakanishi; Kanehisa Yokoyama; 久雄 中西; 兼久 横山
Archive | 2007
Kenji Kinoshita; Kanehisa Yokoyama; Kentaro Fujimoto; Toru Yakabe; Shin Saito; Kazuhiko Fujiwara
Archive | 2008
Hajime Fukunaga; Kazuhiko Fujiwara; Kanehisa Yokoyama; Kentaro Fujimoto
Archive | 2006
Kenji Kinoshita; Toru Yakabe; Kentaro Fujimoto; Kanehisa Yokoyama; Kazuhiko Fujiwara
Archive | 2006
Kenji Kinoshita; Kazuhiko Fujiwara; Kanehisa Yokoyama; Kentaro Fujimoto; Toru Yakabe
Archive | 2006
Kenji Kinoshita; Kazuhiko Fujiwara; Kanehisa Yokoyama; Kentaro Fujimoto; Toru c; o Sumitomo Bakelite Co. Ltd. Yakabe
Analytical Sciences | 2009
Atsuko Tanaka; Naoki Harikai; Shin Saito; Toru Yakabe; Sohei Funaoka; Kanehisa Yokoyama; Kazuhiko Fujiwara; Kyoko Iwao-Koizumi; Shigenori Murata; Kenji Kinoshita
Archive | 2007
Kanehisa Yokoyama; Kenji Kinoshita; Kentaro Fujimoto; Toru Yakabe; Shin Saito