Kartika Dewi

Two new species of philometrid nematodes (Nematoda: Philometridae) in Epinephelus coioides (Hamilton, 1822) from the South Bali Sea, Indonesia

Based on light and scanning electron microscopy, two new species of philometrid nematodes, Spirophilometra endangae sp. nov. and Philometra epinepheli sp. nov. (Nematoda: Dracunculoidea: Philometridae) are described from Epinephelus coioides (Hamilton, 1822) (Perciformes: Serranidae) from the South Bali Sea, Indonesia. Spirophilometra endangae sp. nov. was isolated from the fins of E. coioides. The new species can be distinguished from the most closely related S. eichleri Parukhin, 1971 by a larger total body length and the site of infection in the host. The new species differs from S. centropomi (Caballero, 1974) also in the larger body size of the gravid females and the site of infection in the host. S. en-dangae sp. nov. differs from S. pacifica (Moravec, Santana-Pineros, Gonzales-Solis & Torres-Huerta, 2007) in the struc-ture and arrangement of the spines on the middle part of the body, the infection site of the worm, the type host and the zoogeographical host distribution. Philometra epinepheli sp. nov. differs from all other Philometra spp. congeners so far recorded from Ephinepelus groupers in the total body length and the site of infection. This is the first opercula-infecting species of Philometra described from the fish family Serranidae.

A NEW SYPHACIA SPECIES (NEMATODA: OXYURIDAE) COLLECTED FROM BUNOMYS SPP. (RODENTIA: MURIDAE) IN CENTRAL SULAWESI, INDONESIA

Abstract Syphacia (Syphacia) rifaii sp. n. (Nematoda: Oxyuridae) is described from endemic Bunomys chrysocomus and Bunomys prolatus (Rodentia: Muridae) on Sulawesi Island, Indonesia. The new species is closest morphologically to Syphacia (Syphacia) sulawesiensis, parasitic in Rattus xanthurus from Sulawesi Island, by having large vesicular lateral alae in males, but is readily distinguished by having a smaller body, a round cephalic plate in both sexes, the absence of lateral alae in females, a longer relative distance between excretory pore and vulva, and smaller eggs. Syphacia (S.) rifaii is surmised to be a specific parasite of Bunomys spp. and has evolved from a common ancestor with S. (S.) sulawesiensis on Sulawesi Island.

Syphacia (Syphacia) Semiadii N. SP. (Nematoda: Oxyuridae) from Halmaheramys Bokimekot Fabre et al., 2013 (Rodentia: Muridae) on Halmahera Island, Indonesia and A Key to The Species Present in Sulawesi and The Australian Bioregion

Abstract Syphacia (Syphacia) semiadii n. sp.is described from Halmaheramys bokimekot Fabre et al., 2013, an endemic murine of Halmahera Island, Maluku, Indonesia. Among the New Guinea/Australian congeners, S. (S.) semiadii n. sp. most closely resembles S. (S.) mamelonitenuis and S. (S.) longaecauda in having a circular cephalic plate without dorsoventral constriction laterally and lacking lateral alae in both sexes. However, this species differs from S. (S.) mamelonitenuis, which has a shorter tail in both sexes and larger eggs, and from S. (S.) longaecauda, which has a longer tail in both sexes. to aid identification, we also provide a dichotomous key to the species of Syphacia in Sulawesi and the Australian bioregion.

Philometrid nematodes (Philometridae) of marine teleosts from Balinese waters, Indonesia, including the description of Philometra damriyasai sp. nov.

Based on light and scanning electron microscopy, one new species of philometrid nematodes, Philometra damriyasai sp. nov. (Nematoda: Dracunculoidea: Philometridae) from the body cavity of Tylerius spinosissimus (Regan, 1908) (Tetraodontiformes, Tetraodontidae), and five known species are reported in different marine teleosts from South Bali, Indonesia. The new species can be distinguished from the most closely related P. javensis Moravec, Walter & Juniar, 2012 by the absence of sclerotized denticles on the cuticle of the anterior oesophageal lobes of the mouth and smaller larvae. Philometra nemipteri Luo, 2001 of Nemipterus japonicus (Bloch, 1791), P. otolithi Moravec & Manoharan, 2013 of Otolithes ruber (Bloch & Schneider, 1801) (both from gonads) and Philometroides marinus Moravec & Buron, 2009 from the body cavity of Rachycentron canadum (Linnaeus, 1766) are reported for the first time from Indonesian waters, thus extending their range of distribution into the tropical Indo-Pacific Ocean. Philometra lobotidis Moravec, Walter & Yuniar, 2012 from the body cavity of Lobotes surinamensis (Bloch, 1790) and P. ocularis Moravec, Ogawa, Suzuki, Miyaki & Donai, 2002 from the eye cavity of Variola louti (Forsskål, 1775) represent new geographical records, and Philometra sp. was recorded for the first time from Cephalopholis sexmaculata (Rüppell, 1830). A total of 14 philometrids have been so far identified from marine fishes in Indonesia.

Nematodes of amphibians from Java, Indonesia, with a description of new species, Meteterakis wonosoboensis n. sp. (Nematoda : Heterakoidea)

During a survey on the parasites of amphibians of Indonesia, toads (30 Bufo melanostictus) and 246 frogs (213 Fejervarya cancrivora, 11 F. limnocharis, 22 Rana macrodon from West Java and 68 F. cancrivora from Central Java) were examined for parasitic nematodes. Three species of nematodes were found and described, i.e. Meteterakis wonosoboensis n. sp. from Fejervaria cancrivora; Meteterakis japonica from Bufo melanostictus, F. cancrivora and F. limnocharis; and Chabaudus sp. from F. cancrivora, F. limnocharis and Rana macrodon. Meteterakis wonosoboensis n. sp. is distinguished from other species of the genus by the length and shape of spicules, the number of caudal papillae, the presence of gubernaculum in male and the presence of vulval flap in female. Bufo melanostictus and Java are recorded as new host and locality for M. japonica, respectively.

A checklist of nematode parasites from Indonesian murids.

A checklist of nematode parasites from Indonesian murids with their geographic distribution is presented. This checklist is compiled from three sources: the catalogue of nematode parasites of Museum Zoologicum Bogoriense (unpublished specimens in the collection), data from our previous research and articles on nematodes of Indonesian murids. This checklist is presented as a list of nematode parasites with host information, and a host list with information on their nematodes. This paper reports 38 nominal species of nematodes and 13 species identified to the generic level only. The nematodes reported comprise 32 genera and 17 families parasitizing 32 species of Indonesian murids.

Two new species of Trichuris (Nematoda: Trichuridae) collected from endemic murines of Indonesia

Two new species of the genus Trichuris (Nematoda: Trichuridae) parasitic in the old endemic murids of Indonesia are described: T. musseri sp. nov. from Echiothrix centrosa (Murinae: Rattini) in Sulawesi and T. mallomyos sp. nov. from Mallomys rothschildi (Murinae: Hydromyini) in Papua Indonesia. Both species are characterized by having a gradually tapered and sharply pointed distal end of the spicule, being readily distinguished from most of the congeners known from murid rodents. Trichuris musseri is readily distinguished from T. mallomyos by having a much smaller body and large number of nuclei per subdivision of stichosome. The resemblance in spicule morphology between the two new species is of special interest because both hosts belong to different tribes and have different habitats and habits. It remains to be elucidated whether the resemblance is merely homoplasy or actually reflects close phylogenetic relationship of the parasites.

Construction and Periplasmic Expression of the Anti-EGFRvIII ScFv Antibody Gene in Escherichia coli

In the previous study, we constructed an expression vector carrying the anti-EGFRvIII scFv antibody gene with VH-linker-VL orientation. The proteins were successfully produced in the periplasmic space of Escherichia coli. In this study, we substituted the inserted DNA with VL-linker-VH orientation of the anti-EGFRvIII scFv gene and analyzed its expression in E. coli. The DNA fragment was amplified from its cloning vector (pTz-rscFv), subsequently cloned into a previous expression vector containing the pelB signal sequence and his-tag, and then transformed into E. coli TOP10. The recombinant plasmids were characterized by restriction, PCR, and DNA sequencing analyses. The new anti-EGFRvIII scFv antibody proteins have been successfully expressed in the periplasmic compartment of E. coli Nico21(DE3) using 0.1 mM final concentration of IPTG induction. Total proteins, soluble periplasmic and cytoplasmic proteins, solubilized inclusion bodies, and extracellular proteins were analyzed by SDS-PAGE and Western Blot analyses. The results showed that soluble scFv proteins were found in all fractions except from the cytoplasmic space.

Syphacia (Syphacia) maxomyos sp. n. (Nematoda: Oxyuridae) from Maxomys spp. (Rodentia: Muridae) from Sulawesi and Sumatra, Indonesia

The present report describes Syphacia (Syphacia) maxomyos sp. n. (Nematoda: Oxyuridae) from two species of spiny rats, Maxomys musschenbroekii from Sulawesi and M. whiteheadi from Sumatra. It is characterized by a cephalic plate extending laterally with dorsoventral constriction and stumpy eggs with an operculum rim reaching pole. It is readily distinguishable by the former feature from all of hitherto known representatives of this genus in Indonesia, but it resembles parasites in Murini and Hydromyni rodents in continental Asia and Sahul. This is the first Syphacia species distributed in both the Sunda Shelf and Sulawesi with the exception of Syphacia muris, a cosmopolitan pinworm found in rodents of the of genus Rattus. It is surmised that S. maxomyos is specific to Maxomys and that it was introduced to Sulawesi by dispersal of some Maxomys from the Sunda Shelf.

Redescription of Subulura ( Murisubulura ) andersoni (Cobbold, 1876) (Nematoda: Subuluridae) from Bunomys spp. (Rodentia: Muridae) of Sulawesi, Indonesia, with special reference to S . ( M .) suzukii and other related species in the adjacent areas

Subulura (Murisubulura) andersoni (Cobbold, 1876) (Nematoda: Subuluridae) is redescribed in detail using light and scanning electron microscopy based on materials collected from Bunomys chrysocomus and Bunomys andrewsi (Rodentia: Muridae) of Sulawesi, Indonesia, to provide basic reference data for identification. Comparison was made among congeners from adjacent areas, especially Subulura (Murisubulura) suzukii Yagi and Kamiya, 1981 of Japan. Because morphological differences distinguishing S. (M.) andersoni from S. (M.) suzukii and S. (M.) sipiroki were only the thickness of female tail and length of male tail, respectively, further study using DNA sequence analysis is necessary to prove their distinctness.