Karunakar Rao Kudle

MICRO WAVE ASSISTED GREEN SYNTHESIS OF SILVER NANOPARTICLES USING BOSWELL IA SERRATA FLOWER EXTRACT AND EVALUATION OF THEIR ANTIMICROBIAL ACTIVITY

In the present investigation , we describe a cost effective green synthesis of silver nanoparticles from the extract of Boswellia serrata flow er as reducing agent. The formed s ilver nanoparticles were characterized using UV – Vis absorption spectroscopy, Fourier Transfer Infra Red and Scanning Electron Microscope (SEM). The functional group responsible for Ag 0 reduction was found to be alkanoid compounds from FTIR. The SEM analysi s showed the size of nanoparticles to be between 60 - 84 nm. Antimicrobial activity of the formed nanoparticles exhibited maximum activity against gram negative bacteria than gram positive bacte ria. This is for the first report on the use of Boswellia s errat a flower for the synthesis of silver nanoparticles .

Synthesis and biological evaluation of new 2-(6-alkyl-pyrazin-2-yl)-1H-benz[d]imidazoles as potent anti-inflammatory and antioxidant agents

A novel series of 2-(6-alkyl-pyrazin-2-yl)-1H-benzo[d]imidazole analogs (3a–3j) derived from 2-(6-chloropyrazin-2-yl)-1H-benzo[d]imidazole (2) by reacting with various substituted cyclic and acyclic secondary amines in presence of Pd-PEPPSI Mes catalyst by using Buchwald–Hartwig amination in excellent yield. The structures of newly synthesized compounds were elucidated by Fourier transform infrared spectroscopy, 1H-nuclear magnetic resonance, 13C-nuclear magnetic resonance, electrospray ionisation mass spectrometry and high-resolution mass spectrometry spectral data. All the title compounds (3a–3j) were evaluated for in vitro screening against cyclooxygenase-1 and cyclooxygenase-2 by colorimetric COX (human ovine) inhibition assay and in vivo anti-inflammatory activity against cyclooxygenase-2 enzyme by means of the carrageenan-induced rat paw edema. The compound 3i was found to have potent anti-inflammatory activity than standard Ibuprofen. The compounds 3a, 3e, and 3j showed appreciable activity against cyclooxygenase-2 enzyme. However the compounds 3a, 3e, 3g, 3h, and 3j were showed maximum activity within 15 to 24 h and 3g and 3i were showed good DPPH scavenging activity. Moreover, the docking studies were also performed and the results are well agreement with the biological data, suggested that compound 3i was a potential anti-inflammatory agent for further development.