Katarzyna Piekarska

Application of semipermeable membrane devices for long-term monitoring of polycyclic aromatic hydrocarbons at various stages of drinking water treatment

The primary goal of the presented study was the investigation of occurrence and concentration of sixteen selected polycyclic aromatic hydrocarbons in samples from various stages of water treatment and verification of the applicability of semi-permeable membrane devices in the monitoring of drinking water. Another objective was to verify if weather seasons affect the concentration and complexity of PAHs. For these purposes, semipermeable membrane devices were installed in a surface water treatment plant located in Lower Silesia (Poland). Samples were collected monthly over a period of one year. To determine the effect of water treatment on PAH concentrations, four sampling sites were selected: raw water input, a stream of water in the pipe just before ozonation, treated water output and water after passing through the distribution system. After each month of sampling, SPMDs were exchanged for fresh ones and prepared for instrumental analysis. Concentrations of polycyclic aromatic hydrocarbons were determined by high-performance liquid chromatography (HPLC). The presented study indicates that semipermeable membrane devices can be an effective tool for the analysis of drinking water, in which organic micropollutants occur at very low concentrations.

Genotoxic and cytotoxic properties of PM2.5 collected over the year in Wrocław (Poland)

In the ambient is >2000 chemical substances, some of them are absorbed on the surface of the particulate matter and may causes many health problems. Air pollution is responsible for >3.2 million premature deaths which classifies it as a second place environmental risk factor. Especially dangerous for health are polycyclic aromatic hydrocarbons and their derivatives which shows mutagenic and carcinogenic properties. Air pollutions were also classified by International Agency for Research on Cancer to group which carcinogenic properties on human were proved by available knowledge. Air pollutions, are one of the biggest problem in Polish cities. The article presents results of mutagenicity, genotoxicity and cytotoxicity researches conducted on a particulate matter fraction 2.5 μm collected during all year long in Wroclaw agglomeration (Poland). The material was collected on filters using high-flow air aspirator and extracted using dichloromethane. Additionally it was fractionated into 4 parts containing: all pollutants, only polycyclic aromatic hydrocarbons, nitro derivatives of PAHs and dinitro derivatives of PAHS. Dry residue of this fraction was dissolving in DMSO and tested using biological methods. Biological methods include mutagenicity properties which are investigated by Salmonella assay (Ames assay). Other biological method was comet assay and 4 parameter cytotoxicity test PAN-I assay. Results of the conducted experiments show differences in mutagenic, genotoxic and cytotoxic properties between seasons of collection and between volumes of dust pollutions fractions. The worst properties shows particles collected in autumn and winter season Results showed also some correlations in results obtained during different methods and properties. Due to the limited possibilities of testing all chemical compounds present in the PM2.5 fraction, it is recommended to carry out tests based on a set of genotoxic and cytotoxic tests, which is confirmed by the conducted research.