Katell Mevel

257: The Phosphodiesterase Type 4 Inhibitor, Rolipram, is More Efficient to Relax Detrusor Smooth Muscle in Rats with Overactive Bladder than in Control Rats

PDE4 inhibitors deserve further investigation for the treatment of OAB. Bladder strip preparation After 6 weeks of partial bladder outlet obstruction, rats were anesthetized with isoflurane. Entire bladders were excised and placed in Krebs-HEPES buffer (with the following millimolar composition : NaCl 118.0; KCl 4.7; MgSO4 1.2; KH2PO4 1.2; CaCl2 2.5; NaHCO3 4.2; glucose 11.1; HEPES 20.8; pH 7.4). The bladder dome was isolated from the trigone region and four longitudinal strips were then mounted isometrically in 5 ml organ baths filled with Krebs-HEPES buffer maintained at 37°C and continuously bubbled with 95%O2-5%CO2.

999 THE PHOSPHODIESTERASE TYPE 4 INHIBITOR, ROLIPRAM, IS MORE EFFICIENT TO RELAX DETRUSOR SMOOTH MUSCLE IN RATS WITH OVERACTIVE BLADDER THAN IN CONTROL RATS

Hypothesis / aims of study Detrusor smooth muscle relaxation is mainly mediated by the cyclic adenosine monophosphate (cAMP) pathway. Elevation of cAMP levels by phophodiesterase type 4 inhibition relaxes smooth muscle of various origins. The aim of this study was to evaluate the effect of the PDE4 inhibitor rolipram on detrusor smooth muscle tone in a rat pathological model of overactive bladder (OAB) i.e with partial bladder outlet obstruction (BOO).