Katiane M. Ferreira

Estrutura e composição da ictiofauna de riachos do rio Paranapanema, Sudeste e Sul do Brasil

Seventeen 100 m long streams stretches, none of an order higher than three, were sampled to both sides of the main channel of Rio Paranapanema in the States of Sao Paulo and Parana, southeastern and southern Brazil. Each stream stretch had its midpoint located with a GPS satellite receiver and had its fish fauna sampled via a standardized environmental data and fish collection methodology (primarily utilizing electrofishing) with the aim of providing the following information about each stream: 1) the taxonomic composition of the fish fauna and the contribution of each species in that stream in terms of both number of individuals and biomass; 2) a photographic documentation of the live coloration of representative speci-mens of each collected species; and 3) the description of each sampled environment, with colored photographic illustra-tions and details of the main biotic and abiotic parameters. Overall 3,683 fishes were collected, belonging to six orders, 16 families, 37 genera, and 52 species, with a total biomass of 16.8 kg. Of the collected species, approximately 36% were Siluriformes, 36% Characiformes, 11% Gymnotiformes, 10% Perciformes, 4% Cyprinodontiformes, and 2% Synbranchiformes. The most abundant species in terms of total number of individuals were Astyanax altiparanae (15.2%) and Astyanax sp. 1 (12.3%); The species with the largest biomasses were Astyanax altiparanae (28%) and Geophagus brasiliensis (13%). In terms of abundance and biomass collected for each family, the Characidae was clearly the predominant family followed by the Loricariidae, Pimelodidae, and Cichlidae. Among the sampled stream stretches, locality 14 with 24 species and locality 13 with five species yielded the highest and lowest richness in terms of species numbers, respectively. This coincides with the values obtained for the Shannon-Wienner index of specific diversity (H´ = 0.99 and 0.32, respectively). The median species richness for all streams stretches was 11. In the species richness estimate by extrapolation for all 17 sampled stream stretches, a value of 69 species was obtained (with a standard error of four) indicating the need for an additional moderate sampling effort to reach the asymptote of the curve. Of the 52 collected species, eight (approximately 15% of the total) are clearly new to science and five other species (approximately 10% of the total) are of indefinite taxonomic status and require further analysis. Three of the captured species are introduced (approximately 6% of the total). Analysis of the trophic and spatial structure of the studied fish fauna indicates that the 10 numerically dominant species in the sampled streams can be grouped into four guilds that are in decreasing order of numeric importance: nektonic omnivores; benthonic invertivores; periphytovores; and benthonic omnivores. An identification key for all the species of fish collected during this study it is provided.

Biology and ecomorphology of stream fishes from the rio Mogi-Guaçu basin, Southeastern Brazil

The corrego Pauliceia is a direct tributary of the rio Mogi-Guacu, located in Santa Rita do Passa Quatro, State of Sao Paulo, Southeastern Brazil. This stream runs inside a savannah-like (cerrado) environment and it associated vegetation. Biological and ecomorphological aspects of its fish community were studied in three stretches of the stream, denominated herein as the upper, middle and lower courses. The fish fauna recorded in this study consisted of 15 species, belonging to five orders and nine families, based in a total of 715 collected individuals representing 1,450 kg of biomass. The most important abiotic factors related to the occurrence and distribution of the fish fauna were the substrate, current speed, and presence or absence of riparian vegetation. The analysis of 336 stomach contents of 13 species demonstrated that 65% of food items are autochthonous, 32% allochthonous, and 3% are of unknown origin. Four alimentary guilds could be identified, based on the predominant food items: omnivores with tendency to insectivory - Astyanax paranae, A. fasciatus and Piabina argentea; larvivores - Corydoras difluviatilis, Cetopsorhamdia iheringi, Phenacorhamdia tenebrosa, Characidium gomesi, Eigenmannia virescens, and Rhamdia quelen; periphyton feeders - Hisonotus sp., Hypostomus ancistroides and Synbranchus marmoratus; and piscivore - Hoplias malabaricus. Reproductions data are presented for Astyanax altiparanae and Hisontus sp. Three ecomorphological groups could be established, based on swimming, feeding, and microhabitat strategies: nektonic (A. fasciatus, A. paranae, Bryconamericus stramineus, P. argentea, H. malabaricus and Phallotorynus jucundus), benthic (C. gomesi, P. tenebrosa, C. iheringi, R. quelen, C. difluviatilis, H. ancistroides and Hisonotus sp.), and necktobenthic (E. virescens and S. marmoratus).

Spermatic characteristics and sperm evolution on the subfamily Stevardiinae (Ostariophysi: Characiformes: Characidae)

The monophyly and phylogenetic relationships among the members of Clade A characids (sensu Malabarba & Weitzman), later redefined and named as the Stevardiinae (sensu Mirande), have been primarily supported by traditional morphological and molecular data. Herein were examined, described and compared spermiogenesis and sperm ultrastructure of 12 species of the genera Boehlkea, Bryconacidnus, Bryconamericus, Creagrutus, Cyanocharax, Hemibrycon, Knodus, Odontostoechus, Piabina, and Rhinobrycon in order to evaluate possible phylogenetic signals and their potential use in recovering relationships of the Stevardiinae. All examined species demonstrated a nuclear rotation equal or less than 95o resulting in a lateral position of the double nuclear fossa and flagellum. In all species, sperm nuclei are slightly elongate toward the flagellum, the proximal centriole is partially inside the nuclear fossa and lies anterior and oblique to the distal centriole, and the midpiece is short and strongly asymmetric. All species analyzed herein and other species previously examined for these systems in the Stevardiinae share homologous sperm characteristics as evidenced by spermiogenesis, further supporting the monophyly of this clade. Spermatozoa of the Stevardiinae further show three morphotypes (M1, M2, M3) of arrangement of centrioles, flagellum, nucleus and midpiece, hypothesized as successively derived in a series of transformation from the most basal morphotype (M1).

A new genus and two new species of Stevardiinae (Characiformes: Characidae) with a hypothesis on their relationships based on morphological and histological data

Lepidocharax, new genus, and Lepidocharax diamantina and L. burnsi new species from eastern Brazil are described herein. Lepidocharax is considered a monophyletic genus of the Stevardiinae and can be distinguished from the other members of this subfamily except Planaltina, Pseudocorynopoma, and Xenurobrycon by having the dorsal-fin origin vertically aligned with the anal-fin origin, vs. dorsal fin origin anterior or posterior to anal-fin origin. Additionally the new genus can be distinguished from those three genera by not having the scales extending over the ventral caudal-fin lobe modified to form the dorsal border of the pheromone pouch organ or to represent a pouch scale in sexually mature males. In this paper, we describe these two recently discovered species and the ultrastructure of their spermatozoa.

Diet of Crenicichla britskii (Perciformes: Cichlidae) in a stream of Rio Aguapeí basin, Upper Rio Paraná system, southeastern Brazil

We qualitatively studied the gut contents of 20 specimens (36.1-71.3 mm standard length) of the pike cichlid Crenicichla britskii from a 100 m stretched second order stream running inside a small gallery forest in a large pasture area of Rio Aguapei basin, State of Sao Paulo, southeastern Brazil. The diet, in terms of per cent composition, constituted primarily of aquatic insects (50%) (mostly immature forms), crustaceans (14.3%), unidentified organic material and filamentous green algae (both with 10.7%), followed by fishes and arachnids (both with 7.1%). Diet analysis indicates that C. britskii is a generalist insectivore with a relatively short digestive tube, which feeds on autochthonous items captured in a broad array of habitats such as in the water column or at the surface, buried in or over the bottom, or associated with macrophytes and submerged vegetation debris.

Knodus shinahota (Characiformes: Characidae) a new species from the río Shinahota, río Chapare basin (Mamoré system), Bolivia

Knodus shinahota, new species, is described from rio Shinahota, a tributary of the upper rio Mamore basin, Provincia de Tiraque, Cochabamba State, Bolivia. The new species can be distinguished from all congeners except K. chapadae and K. geryi by having six rows of scales between the lateral line and the dorsal-fin origin (vs 4 or 5 rows of scales in the other species). Knodus shinahota differs from K. chapadae by possessing more rows of scales between the lateral line and the pelvic-fin origin (5 vs 3½ or 4, respectively) and more lateral line scales (38-41 vs 36-38, respectively). It differs from K. geryi by possessing more rows of scales between the lateral-line and the pelvic-fin origin (5 vs 4, respectively); fewer branched anal-fin rays (17-20 vs 15-17, respectively), and by lacking the two symmetric, large, dark, blotches on the basal portions of the caudal-fin lobes that characterize K. geryi.

A New Species of Knodus (Characiformes: Characidae) from the Rio Tiquie ´, Upper Rio Negro System, Brazil

Abstract Knodus tiquiensis, new species, is described from Rio Tiquié, a tributary of the Rio Uaupés, upper Rio Negro system, Amazon basin, Brazil. The new species is distinguished from its congeners by the presence of thickened skin forming folds that cover the basal portion of the six anteriormost branched anal-fin rays and its interradial membrane. Also, the anteriormost six anal-fin rays are considerably thicker than remaining rays of the fin. Comparisons to Knodus tiquiensis with its congeners are presented based on an examination of type material of all species currently included in the genus. Knodus tiquiensis, espécie nova, é descrita do Rio Tiquié, um tributário do Rio Uaupés, alto Rio Negro, Amazonas, Brasil. A nova espécie pode ser distinguida de suas congêneres pela presença de uma pele espessada entre os raios anteriores da nadadeira anal, formando dobras mais desenvolvidas na base e membranas inter-radiais dos seis primeiros raios ramificados e por possuir tais raios consideravelmente mais espessos que os demais. Comparações entre Knodus tiquiensis com seus congêneres são apresentadas, baseadas em exame do material tipo de todas as espécies atualmente incluídas no gênero Knodus.

A new species of Bryconadenos (Characiformes: Characidae) from the rio Curuá, rio Xingu drainage, Brazil

A second inseminating species bearing club cells organized into an anal-fin gland in sexually active males belonging to the genus Bryconadenos was recently discovered in the rio Xingu drainage and is described as new. It is distinguished from B. tanaothoros by the presence of a conspicuous dark blotch at the humeral region, and fewer scale rows between dorsal-fin origin and anal-fin origin. Males of B. weitzmani have longer pelvic fins at sizes beyond 30 mm SL. Bryconadenos weitzmani is further distinguished from B. tanaothoros by the cup shape of the anal-fin gland, its lower part much more developed than the upper, whereas in B. tanaothoros there is just a slit separating the almost equally developed upper and lower parts.