Katsura Funayama

Antihypertensive Effects of Hydrolysates of Wakame (Undaria pinnatifida) and Their Angiotensin-I-Converting Enzyme Inhibitory Activity

Aim: The angiotensin-I-converting enzyme (ACE) inhibitory and antihypertensive activities of wakame hydrolysates have been investigated in several studies. Methods: Wakame (Undaria pinnatifida) was hydrolyzed using 17 kinds of proteases and the inhibitory activity of the hydrolysates for ACE was measured. Of these hydrolysates 4 with potent ACE inhibitory activity were administered singly and orally to spontaneously hypertensive rats (SHR). Results: The systolic blood pressure of SHR decreased significantly after single oral administration of protease S ‘Amano’ and proleather FG-F hydrolysates (10 mg protein/kg body weight). In a long-term feeding experiment, 7-week-old SHR were fed standard chow supplemented with protease S ‘Amano’-derived wakame hydrolysates for 10 weeks. In SHR fed the 1 and 0.1% wakame hydrolysates, elevation of systolic blood pressure was still significantly suppressed for 7 weeks. Conclusions: The hydrolysates derived from wakame by protease S ‘Amano’ have a powerful ACE-inhibitory activity (IC50 = 86 µg protein/ml) and were effective in spite of their slight bitterness as ‘physiologically functional food’ with antihypertensive activity.

Acknowledgement to the 2002 Reviewers

Ambrosini, G., Crawley, Australia Azizi, F., Tehran, Iran Baker, P.W., Adelaide, Australia Baumgartner, M., Basel, Switzerland Berg, A., Freiburg, Germany Böhles, H.J., Frankfurt, Germany Bitsch, I., Giessen, Germany Bode, C., Stuttgart, Germany Boeing, H., Bergholz-Rehbrücke, Germany Bretillon, L., Dijon, France Brussaard, J.H., Zeist, The Netherlands Burdan, F., Lublin, Poland Chango, A., Jefferson, USA Cser, A., Budapest, Hungary Cynober, L.A., Paris, France Demirkol, M., Istanbul, Turkey Donaldson, M., Salisbury, USA Elmadfa, I., Vienna, Austria Faist, V., Kiel, Germany Flachowsky, G., Braunschweig, Germany Fogliano, V., Portici, Italy Gazia, N., Assiut, Egypt Genser, D., Vienna, Austria Grandjean, A., Omaha, USA Koletzko, B., Munich, Germany König, J., Vienna, Austria Kosenko, E., Pushchino, Russia Krawinkel, M., Giessen, Germany Lohninger, A., Vienna, Austria Moser, U., Basel, Switzerland Münch, G., Leipzig, Germany Nakamoto, T., New Orleans, USA Nestel, P.J., Melbourne, Australia Niedermüller, H., Vienna, Austria Nogala, M., Poznan, Poland Oliver, P., Palma de Mallorca, Spain Oltersdorf, U., Karlsruhe, Germany Palou, A., Palma de Mallorca, Spain Pedersen, J., Oslo, Norway Pokorny, J., Prague, Czech Republic Pollak, A., Vienna, Austria Prentice, A., Cambridge, UK Remer, T., Dortmund, Germany Renaud, S., Bordeaux, France von Ruecker, A., Bonn, Germany Rust, P., Vienna, Austria Sanchez-Muniz, F., Boston, USA Sanders, T., London, UK Schächinger, V., Frankfurt, Germany Scheppach, W., Würzburg, Germany Schiefermeier, M., Vienna, Austria Simopoulos, A., Washington, USA Sjöström, M., Huddinge, Sweden Stehle, P., Bonn, Germany Steinhart, H., Hamburg, Germany Suetsuna, K., Yamaguchi, Japan Tamura, M., Tsukuba, Japan de Vrese, M., Kiel, Germany Wagner, K.-H., Vienna, Austria Wascher, T., Graz, Austria Wenk, C., Zürich, Switzerland Yoshida, A., Nagoya, Japan Zittermann, A., Bonn, Germany Zunft, J., Bergholz-Rehbrücke, Germany Zwiauer, K. F., St. Pölten, Austria