Kazumasa Nakano
Otsuka Pharmaceutical
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Publication
Featured researches published by Kazumasa Nakano.
Analytical Biochemistry | 1992
Noboru Tomiya; Takeshi Suzuki; Juichi Awaya; Kuniharu Mizuno; Akira Matsubara; Kazumasa Nakano; Masayasu Kurono
A sensitive and simple high-performance liquid chromatographic method has been developed to determine the concentration of monosaccharides and sugar alcohols in animal tissues. Five neutral monosaccharides (D-glucose, D-galactose, D-mannose, D-fructose, and D-ribose) and three neutral sugar alcohols (myo-inositol, glycerol, and D-sorbitol) predominate in the renal cortices and sciatic nerves of rats. These monosaccharides and sugar alcohols were extracted with distilled water, purified by deproteinization with ethanol, a Sep-Pak C18 cartridge, and columns of Dowex 50W-X8 and Amberlite CG-400, then separated on Ca2+ and Pb2+ cation-exchange columns, eluted with deionized distilled water at 80 degrees C, and detected using integrated pulsed amperometry. About 10 pmol of each sugar was detectable with a signal-to-noise ratio of 10:1. D-Glucose, D-fructose, D-sorbitol, and D-mannose were higher in both the renal and sciatic tissues of diabetic rats than in those of normal animals. D-Ribose and glycerol were higher in the renal cortex of diabetic animals.
Journal of Diabetes and Its Complications | 1994
Noriaki Kato; Kuniharu Mizuno; Akira Matsubara; Kazumasa Nakano; Masayasu Kurono; Soroku Yagihashi
Abstract We studied the long-term effects of a new aldose reductase inhibitor, (2 S ,4 S )-6-fluoro-2′,5′-dioxospiro-[chroman-4,4′-imidazolidine]-2-carboxamide (SNK-860), on functional, biochemical, and structural changes in peripheral nerve of streptozotocin (STZ)-induced diabetic rats. During the experimental period of 26 weeks, the delayed motor-nerve conduction in diabetic rats was significantly prevented by SNK-860 treatment, and elevated sorbitol levels and reduced myo -inositol levels were normalized to 100% and 71% of control levels, respectively. Teased nerve fiber studies demonstrated that the frequency of abnormal fibers was significantly reduced in treated diabetic rats. Morphometric analysis of myelinated fibers also disclosed prevention of axonal atrophy, distorted axonal circularity and preservation of large-sized fibers following SNK-860 treatment. These results suggest that long-term treatment with SNK-860 has a beneficial preventive effect on the development of experimental diabetic neuropathy.
Metabolism-clinical and Experimental | 1992
Kuniharu Mizuno; Noriaki Kato; Akira Matsubara; Kazumasa Nakano; Masayasu Kurono
Archive | 1989
Kiichi Sawai; Masayasu Kurono; Hiromoto Asai; Takahiko Mitani; Kazumasa Nakano; Naohisa Ninomiya
Acta Medica Okayama | 1993
Masahiko Akita; Kuniharu Mizuno; Akira Matsubara; Kazumasa Nakano; Masayasu Kurono
Archive | 1994
Masahiko Akita; Masatsune Kurono; Akira Matsubara; Kuniharu Mizuno; Kazumasa Nakano; 万正 中野; 旭 松原; 邦治 水野; 昌彦 秋田; 昌庸 黒野
Journal of Toxicological Sciences | 1994
Kyoichi Asano; Masaya Yamano; Kiyoshi Haruyama; Etuo Ikawa; Kazumasa Nakano; Masayasu Kurono; Osamu Wada
Archive | 1989
Kiichi Sawai; Masayasu Kurono; Hiromoto Asai; Takahiko Mitani; Motohide Hayashi; Kazumasa Nakano; Naohisa Ninomiya
Archive | 1992
Kiichi Sawai; Masayasu Kurono; Kazumasa Nakano; Kyoichi Asano; Takahiko Mitani; Naohisa Ninomiya; Bunkichi Kato
Chemical & Pharmaceutical Bulletin | 1989
Mikio Hori; Tadashi Kataoka; Hiroshi Shimizu; Eiji Imai; Noriyuki Iwata; Norihiro Kawamura; Masayasu Kurono; Kazumasa Nakano; Masaru Kido