Kazuo Ikegaya
Green Cross International
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Publication
Featured researches published by Kazuo Ikegaya.
Biochimica et Biophysica Acta | 1994
Hiroki Tatsumi; Kazuo Ikegaya; Seiji Murakami; Haruhide Kawabe; Eiichi Nakano; Hiroshi Motai
The neutral proteinase II from Aspergillus oryzae (NpII) is a zinc proteinase with three intramolecular disulfide bonds. NpII is most unstable after 10 min at about 75 degrees C, but regains stability beyond this temperature and is relatively stable at 100 degrees C. We analyzed the thermal stability of wild-type NpII and apo NpII. The results suggested that NpII unfolds reversibly upon incubation up to 100 degrees C, and that the irreversible inactivation observed is mainly due to autoproteolysis. To further understand the stability, a mutant NpII (Cys78-->Ala) lacking one of the disulfide bonds, was produced in a heterologous yeast expression system. The mutant NpII showed a similar stability profile, but the most unstable temperature and the most catalytically active temperature decreased to the same extent (around 10 degrees C), confirming that autoproteolysis is the main cause of the irreversible inactivation. Several lines of evidence presented in this study demonstrated that the thermal stability of o++NpII is attributed to reversible thermal unfolding and autoproteolysis.
Archive | 1995
Naofumi Hayasuke; Yukimitsu Nakagawa; Yutaka Ishida; Ken Okabayashi; Kohji Murakami; Kiyoshi Tsutsui; Kazuo Ikegaya; Hitoshi Minamino; Sadao Ueda; Haruhida Kawabe; Hirofumi Arimura; Atusi Masaki
Archive | 1989
Yukimitsu Nakagawa; Naofumi Hayasuke; Atsushi Masaki; Yutaka Ishida; Kohji Murakami; Ken Okabayashi; Kiyoshi Tsutsui; Hitoshi Minamino; Kazuo Ikegaya; Kaoru Kobayashi; Haruhide Kawabe; Sadao Ueda
Archive | 1989
Yukimitsu Nakagawa; Naofumi Hayasuke; Atsushi Masaki; Yutaka Ishida; Kohji Murakami; Ken Okabayashi; Kiyoshi Tsutsui; Hitoshi Minamino; Sadao Ueda; Kazuo Ikegaya; Kaoru Kobayashi; Haruhide Kawabe
Bioscience, Biotechnology, and Biochemistry | 1992
Kazuo Ikegaya; Yutaka Ishida; Kohji Murakami; Atsushi Masaki; Naritoshi Sugio; Kazuo Takechi; Seiji Murakami; Hiroki Tatsumi; Yoshihiro Ogawa; Eiichi Nakano; Hiroshi Motai; Haruhide Kawabe
Biotechnology and Bioengineering | 2003
Kazuo Ikegaya; Shigetoshi Sugio; Kohji Murakami; Kouichi Yamanouchi
Archive | 1988
Naofumi Hayasuke; Kazuo Ikegaya; Yutaka Ishida; Haruhide Kawabe; Kaoru Kobayashi; Koji Mazaki; Hitoshi Minamino; Koji Murakami; Yukimitsu Nakagawa; Ken Okabayashi; Kiyoshi Tsutsui; Sadao Ueda
Archive | 1988
Hirobumi Arimura; Naofumi Hayasuke; Kazuo Ikegaya; Yutaka Ishida; Haruhide Kawabe; Koji Mazaki; Hitoshi Minamino; Koji Murakami; Yukimitsu Nakagawa; Ken Okabayashi; Kiyoshi Tsutsui; Sadao Ueda
Archive | 1989
Yukimitsu Nakagawa; Naofumi Hayasuke; Atsushi Masaki; Yutaka Ishida; Kohji Murakami; Ken Okabayashi; Kiyoshi Tsutsui; Hitoshi Minamino; Sadao Ueda; Kazuo Ikegaya; Kaoru Kobayashi; Haruhide Kawabe
Archive | 1988
Naofumi Hayasuke; Yukimitsu Nakagawa; Yutaka Ishida; Ken Okabayashi; Kohji Murakami; Kiyoshi Tsutsui; Kazuo Ikegaya; Hitoshi Minamino; Sadao Ueda; Haruhida Kawabe; Hirofumi Arimura; Atusi Masaki