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The Journal of the Japanese Association for Infectious Diseases | 1989

Rapid Diagnostic Methods for Fungal Infections-Using Chromogenic Limulus Tests and Enzymatic Measurement of D-Arabinitol

Takashige Miyazaki; Shigeru Kohno; Kazuo Sasayama; Hiroshi Yamada; Akira Yasuoka; Yasumasa Doutsu; Yamaguchi K; Masaki Hirota; Kohei Hara

Conventional chromogenic limulus test (toxicolor test) reacts with glucan of bakers yeast as well as endotoxin, because toxicolor test contains factor G which is sensitive to glucan and polysaccharide of fungus. A new endotoxin specific limulus test (endospecy test) was developed to improve reaction which responded only to endotoxin. The toxicolor test showed the positive reaction to the culture media of candida albicans 7N strain in RPMI 1640 medium, but the endospecy test did not. D-arabinitol, measured by fluometric enzymatic method, also was positive to culture media. These results suggested that a combination of the toxicolor and the endospecy test and enzymatic measurement of D-arabinitol could be applied to rapid diagnostic methods of fungal infection.


The Journal of the Japanese Association for Infectious Diseases | 1986

A case of Staphylococcus aureus septicemia after acupuncture therapy

Yasumasa Doutsu; Tao Y; Kazuo Sasayama; Inoue Y; Yamashita K; Hideaki Shigeno; Miyazaki Y; Hironobu Koga; Nagasawa M; Fukuda Y


Tohoku Journal of Experimental Medicine | 1992

Polysaccharide-Coated Liposomal Amphotericin B for the Treatment of Murine Pulmonary Candidiasis

Takashige Miyazaki; Shigeru Kohno; Kazuo Sasayama; Yuichi Inoue; Kohei Hara; Masayoshi Ogasawara; Toshinori Sato; Junzo Sunamoto


The Journal of the Japanese Association for Infectious Diseases | 1989

An autopsied case of disseminated cryptococcosis with marked lymphadenopathy

Kazuo Sasayama; Oe T; Inuyama M; Tanigawa H; Kawamura K; Akira Yasuoka; Hiroshi Yamada; Dohtsu Y; Toshiaki Hayashi; Shigeru Kohno


The Journal of the Japanese Association for Infectious Diseases | 1986

Experimental Pseudomonas pneumonia model in normal mice

Shigeru Kohno; Kazuo Sasayama; Yasumasa Doutsu; Yamashita K; Shibuya N; Takashige Miyazaki; Hironobu Koga; Nakazato H; Nagasawa M; Naofumi Suyama


The Journal of the Japanese Association for Infectious Diseases | 1992

真菌多糖類によるリムルステスト(factor G)の活性化について

Takashige Miyazaki; Shigeru Kohno; Kotaro Mitsutake; Shigefumi Maesaki; Hiroshi Yamada; Akira Yasuoka; Kazuo Sasayama; Mitsuo Kaku; Hironobu Koga; Hara K


The Journal of the Japanese Association for Infectious Diseases | 1990

家兎カンジダ症におけるLimulus test, D-arabinitol測定及びLatex凝集法の比較検討

Takashige Miyazaki; Shigeru Kohno; Akira Yasuoka; Kazuo Sasayama; Hiroshi Yamada; Yasumasa Dohtsu; Keizo Yamaguchi; Masaki Hirota; Kohei Hara


Kekkaku(Tuberculosis) | 1990

ADVERSE REACTIONS OF ANTITUBERCULOUS AGENTS.

Hiroshi Yamada; Akira Yasuoka; Kazuo Sasayama; Yasumasa Doutsu; Toshiaki Hayashi; Shigeru Kohno; Yamaguchi K; Masaki Hirota; Kohei Hara


Chemotherapy | 1987

Laboratory Studies And Clinical Evaluation Of Carumonam In Respiratory Infections

Kazuo Sasayama; Kyoko Yamashita; Yasumasa Dotsu; Takashige Miyazaki; Hiroko Nakazato; Hideaki Shigeno; Hironobu Koga; Takashi Suyama; Nagasawa M; Kenji Mori; Yoshiaki Fukuda; Toshiaki Hayashi; Naomi Ito; Yoshiteru Shigeno; Keizo Yamaguchi; Masaki Hirota; Atsushi Saito; Kohei Hara; Kazuyuki Sugawara; Kazunori Shimoguchi; Kazunori Tomono; Nobukane Kusano; Yasuharu Masuyama; Toshiyuki Oe; Takeshi Ishizaki

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