Kazutaka Arisue

Relationship between Circadian Rhythm of Food Intake and that of Plasma Corticosterone and Effect of Food Restriction on Circadian Adrenocortical Rhythm in the Rat

The circadian rhythms in both food intake and plasma corticosterone (11-OHCS) in adult female rats were altered concomitantly under various lighting conditions. In all lighting regimens, plasma 11-OHC

Effect of food restriction and its withdrawal on the circadian adrenocortical rhythm in rats under constant dark or constant lighting condition.

Effects of food restriction and its withdrawal on the circadian adrenocortical rhythm were studied in adult female rats under constant darkness (DD) and constant lighting (LL). The animals were fed re

Rapid analysis of serum lactate dehydrogenase isoenzymes by high-performance ion-exchange chromatography

Abstract The repetitive analysis of serum lactate dehydrogenase (LDH) isoenzymes has been performed on a weak anion exchanger (TSKgel DEAE-5PW), which was developed by introducing diethylaminoethyl groups into TSKgel G5000PW (10 μm particle diameter) — a hydrophilic polymer-based material of large pore size — for high-performance gel chromatography. By use of this anion exchanger, a high-pH (8.0) solvent could be used and the albumin peak was completely separate from the LDH isoenzyme peaks. After 10 successive analyses with an autosampler, the coefficient of variation of the LDH isoenzyme elution times was ⩽ 0.90%, and the coefficient of variation for peak areas was ⩽ 3.85%. After 40 successive analyses, resolution between isoenzymes was generally 1.25. This column can be used for more than 300 intermittent injections of human serum.

Nutritional Factors and Atherosclerosis

It is said that the use of foods low in cholesterol content is essential to dietary treatment, because of the close relationship between the serum cholesterol level and the onset or progress of atherosclerosis.

Automated enzymatic determination of serum and urine creatine using the Abbott ABA-200.

A new enzymatic method is described for the determination of creatine in serum and urine with Abbott ABA-200. The measurement is accomplished by transforming creatine to formic acid in a reaction catalyzed by creatinase (creatine amidinohydrolase), sarcosine oxidase and formaldehyde dehydrogenase (see Figure 1). The assay takes less than 20 minutes. The standard response is linear for creatine concentrations up to 10 mg/dL (serum) and 80 mg/dL (urine). The coefficients of variation at 0.69 mg/dL (serum) and 4.93 mg/dL (urine) for within-day determination were less than 4% and, for between-day determination, were less than 5%. Results obtained by this procedure on one hundred serum and urine samples conformed well with a manual enzymatic method and the Folin method. The method is useful for the automated measurement of creatine in serum and urine.