Kazutaka Takahashi

High Ash Content in Net-Plankton Samples from Shallow Coastal Water: Possible Source of Error in Dry Weight Measurement of Zooplankton Biomass

Annual examination of net-plankton biomass in dry weight, ash-free dry weight, organic carbon, and nitrogen weight at the Manazuru harbor, central Japan revealed that net-plankton samples from shallow coastal water contained considerable amount of ash (53±13% of dry weight) which would be caused by contamination of inorganic materials from re-suspendion of sediments, terrestrial runoff and chain-forming diatoms. Therefore, in coastal water, dry weight is inadequate fro determination of zooplankton biomass in volving the possibility of over-estimation. Practical estimation of net-plankton biomass in shallow coastal waters, ash-free dry weight, organic carbon, and/or nitrogen are more adequate.

Nitrification and its influence on biogeochemical cycles from the equatorial Pacific to the Arctic Ocean

We examined nitrification in the euphotic zone, its impact on the nitrogen cycles, and the controlling factors along a 7500 km transect from the equatorial Pacific Ocean to the Arctic Ocean. Ammonia oxidation occurred in the euphotic zone at most of the stations. The gene and transcript abundances for ammonia oxidation indicated that the shallow clade archaea were the major ammonia oxidizers throughout the study regions. Ammonia oxidation accounted for up to 87.4% (average 55.6%) of the rate of nitrate assimilation in the subtropical oligotrophic region. However, in the shallow Bering and Chukchi sea shelves (bottom ⩽67 m), the percentage was small (0–4.74%) because ammonia oxidation and the abundance of ammonia oxidizers were low, the light environment being one possible explanation for the low activity. With the exception of the shallow bottom stations, depth-integrated ammonia oxidation was positively correlated with depth-integrated primary production. Ammonia oxidation was low in the high-nutrient low-chlorophyll subarctic region and high in the Bering Sea Green Belt, and primary production in both was influenced by micronutrient supply. An ammonium kinetics experiment demonstrated that ammonia oxidation did not increase significantly with the addition of 31–1560 nm ammonium at most stations except in the Bering Sea Green Belt. Thus, the relationship between ammonia oxidation and primary production does not simply indicate that ammonia oxidation increased with ammonium supply through decomposition of organic matter produced by primary production but that ammonia oxidation might also be controlled by micronutrient availability as with primary production.

Photodissociation of gas-phase I−3: product branching in the visible and UV regions

Abstract Photodissociation of the gas-phase triiodide anion, I − 3 , was investigated using photofragment mass spectrometry. The photofragment yield (PFY) and photoproduct branching fractions were determined from the observed mass spectra in the wavelength range 270–540 nm (2.3–4.6 eV). The measurement revealed that: 1. I − 3 photodissociation occurs with the production of I − and I − 2 fragments in the whole energy range studied, 2. the branching ratio of I − versus I − 2 production depends strongly on the excitation energy. These experimental findings clearly demonstrate the nonadiabatic nature of the I − 3 photodissociation dynamics.

Predation by scavenging amphipods to injured hatchery-raised juvenile Japanese flounder Paralichthys olivaceus under laboratory conditions

The attacking potential of the scavenging amphipod Scopelocheirus onagawae on artificially injured hatchery-raised Japanese flounder Paralichthys olivaceus juveniles was investigated in relation to the degree of injury on the fish. All injured flounder juveniles were attacked by amphipods regardless of the degree of injury, while non-injured juveniles were not attacked. The attack by amphipods on the juveniles generally depended on the amount of glycine, a main feeding stimulant for the amphipod, released from the injury opening. The swimming ability of flounder juveniles was important to avoid the attack of amphipods. Furthermore, an area of injury allowing the amphipods to cling to the fish affects to the vulnerability of juveniles against the predation of amphipods. This study suggests that scavenging amphipods are potentially involved in the rapid reduction of the number of hatchery-raised juveniles.

Phytoplankton community structure, as derived from pigment signatures, in the Kuroshio Extension and adjacent regions in winter and spring

The relationships between the spatiotemporal variation in phytoplankton community structure and environmental variables were investigated in the Kuroshio Extension (KE) region from winter to spring by analysing biomarker pigments. In winter, when the mixed layer was deep, phytoplankton communities were characterised by low biomass and a relatively high dominance of cryptophytes, followed by chlorophytes and pelagophytes. In spring, phytoplankton biomass generally increased with shoaling of the mixed layer. In April, when nitrate was not exhausted, chlorophytes became the most dominant group throughout the KE region, followed by cryptophytes. In May, in the south of the KE, phytoplankton biomass decreased with the depletion of nitrate and cyanobacteria dominated, whereas at the northern edge of the KE, phytoplankton biomass remained high. A predominance of diatoms occurred sporadically at the northern edge of the first ridge with a shallow mixed layer and an elevated nutricline. In contrast, the contribution of diatoms was low at the northern edge of the second ridge, despite high levels of nitrate and silicic acid, suggesting that factors other than macronutrient depletion limited diatom production. In general, the contribution of diatoms to the total phytoplankton biomass in the KE region was small in both winter (2.9%) and spring (16%). This study showed that the phytoplankton communities in the KE region during the spring bloom were generally composed of non-diatom phytoplankton groups, chlorophytes, cryptophytes, and prasinophytes. It is necessary to identify the roles of non-diatoms in grazing food chains to more accurately evaluate the KE as a nursery area for pelagic fish.

Possible association of diazotrophs with marine zooplankton in the Pacific Ocean.

Dinitrogen fixation, the biological reduction in N2 gas to ammonia contributes to the supply of new nitrogen in the surface ocean. To understand the diversity and abundance of potentially diazotrophic (N2 fixing) microorganisms associated with marine zooplankton, especially copepods, the nifH gene was studied using zooplankton samples collected in the Pacific Ocean. In total, 257 nifH sequences were recovered from 23 nifH‐positive DNA extracts out of 90 copepod samples. The nifH genes derived from cyanobacteria related to Trichodesmium, α‐ and γ‐subdivisions of proteobacteria, and anaerobic euryarchaeota related to Methanosaeta concilii were detected. Our results indicated that Pleuromamma, Pontella, and Euchaeta were the major copepod genera hosting dinitrogen fixers, though we found no species‐specific association between copepods and dinitrogen fixers. Also, the digital PCR provided novel data on the number of copies of the nifH gene in individual copepods, which we report the range from 30 to 1666 copies per copepod. This study is the first systematic study of zooplankton‐associated diazotrophs, covering a large area of the open ocean, which provide a clue to further study of a possible new hotspot of N2 fixation.