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Featured researches published by Kazuya Uenishi.


Nucleic Acids Research | 2005

DNA base flipping by a base pair-mimic nucleoside

Shu-ichi Nakano; Yuuki Uotani; Kazuya Uenishi; Masayuki Fujii; Naoki Sugimoto

On the basis of non-covalent bond interactions in nucleic acids, we synthesized the deoxyadenosine derivatives tethering a phenyl group (X) and a naphthyl group (Z) by an amide linker, which mimic a Watson–Crick base pair. Circular dichroism spectra indicated that the duplexes containing X and Z formed a similar conformation regardless of the opposite nucleotide species (A, G, C, T and an abasic site analogue F), which was not observed for the natural duplexes. The ΔG370 values among the natural duplexes containing the A/A, A/G, A/C, A/T and A/F pairs differed by 5.2 kcal mol−1 while that among the duplexes containing X or Z in place of the adenine differed by only 1.9 or 2.8 kcal mol−1, respectively. Fluorescence quenching experiments confirmed that 2-amino purine opposite X adopted an unstacked conformation. The structural and thermodynamic analyses suggest that the aromatic hydrocarbon group of X and Z intercalates into a double helix, resulting in the opposite nucleotide base flipping into an unstacked position regardless of the nucleotide species. This observation implies that modifications at the aromatic hydrocarbon group and the amide linker may expand the application of the base pair-mimic nucleosides for molecular biology and biotechnology.


Biochemistry | 2009

Dynamics and Energetics of the Base Flipping Conformation Studied with Base Pair-Mimic Nucleosides

Shu-ichi Nakano; Hirohito Oka; Yuuki Uotani; Kazuya Uenishi; Masayuki Fujii; Naoki Sugimoto

A base flipping conformation is found in many biological processes, including DNA repair and DNA and RNA modification processes. To investigate the dynamics and energetics of this unusual conformation in a double helix, base flipping induced by the base pair analogues of deoxyadenosine and deoxycytidine derivatives tethering a phenyl or naphthyl group was investigated. DNA strands bearing the base pair analogues stabilized the base flipping conformation of a complementary RNA, resulting in a site-specific hydrolysis by specific base catalysis. Measurements of the hydrolysis rate and the thermal stability of DNA/RNA duplexes suggested an unconstrained flexibility of the flipped-out ribonucleotide. As established in the base flipping by DNA repair and DNA and RNA modification enzymes, the results suggested that base flipping occurred in competition with base pair formation. In addition, the deoxycytidine derivatives discriminated G from I (inosine), with respect to the base pair interaction energy, as observed for a damaged base or a weakened base pair search by DNA repair proteins. The base pair mimic nucleosides would be useful for investigating the base flipping conformation under the equilibrium with base pairing.


Journal of Polymer Science Part A | 2007

Imidazole‐promoted copolymerization of epoxide and 3,4‐dihydrocoumarin and its application to a high‐performance curing system

Atsushi Sudo; Kazuya Uenishi; Takeshi Endo


Journal of the American Chemical Society | 2005

Site-selective RNA cleavage by DNA bearing a base pair-mimic nucleoside

Shu-ichi Nakano; Yuuki Uotani; Kazuya Uenishi; Masayuki Fujii; Naoki Sugimoto


Journal of Polymer Science Part A | 2008

Anionic alternating copolymerization of 3,4‐dihydrocoumarin and glycidyl ethers: A new approach to polyester synthesis

Kazuya Uenishi; Atsushi Sudo; Takeshi Endo


Journal of Polymer Science Part A | 2009

Anionic alternating copolymerization of a bifunctional six‐membered lactone and glycidyl phenyl ether: Selective synthesis of a linear polyester having lactone moiety

Kazuya Uenishi; Atsushi Sudo; Takeshi Endo


Journal of Polymer Science Part A | 2008

Anionic copolymerization of epoxide with bifunctional aromatic lactone derived from 2‐methylresorcinol

Atsushi Sudo; Kazuya Uenishi; Takeshi Endo


Journal of Polymer Science Part A | 2009

Synthesis of polyester having sequentially ordered two orthogonal reactive groups by anionic alternating copolymerization of epoxide and bislactone

Kazuya Uenishi; Atsushi Sudo; Takeshi Endo


Molecular BioSystems | 2010

Stacking interaction in the middle and at the end of a DNA helix studied with non-natural nucleotides

Shu-ichi Nakano; Hirohito Oka; Yuuki Uotani; Kazuya Uenishi; Masayuki Fujii; Naoki Sugimoto


Journal of Polymer Science Part A | 2005

Novel siloxane‐carrying dithiol derived from 5‐membered cyclic dithiocarbonate and its curing reactions for coating application

Kazuya Uenishi; Atsushi Sudo; Takeshi Endo

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