Kazuyuki Yoshizaki

Establishment of highly sensitive and wide-ranged immunoassay for TNF.ALPHA. by DELFIA.

Non-isotopic immunoassay for the human tumor necrosis factor α (TNFα) was established by employing the dissociated enhanced lanthanide fluoroimmunoassay (DELFIA) system, based on the timeresolved fluoroimmunoassay (TR-FIA) technique utilizing the europium-labeled antibody. In comparison with enzyme-linked immunosorbent assay and bioassay, the sensitivity and range of measurement could be significantly increased by applying the DELFIA system to TNFα.Biological samples, including serum, may affect some of the immunoassays. We overcome the serum influence to the measurement of TNFα by DELFIA, Percent recovery of additional TNFα in each diluted serum was determined, then the sliding standard curve of each serum sample was mathematically calculated from the recovery. Finally corrected TNFα amount was detected by using the sliding standard curve. We suggest that more accurate, sensitive and wide ranged TNFα immunoassay has been established by DELFIA with some revising calculation.