Kei Mukai
Takeda Pharmaceutical Company
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Publication
Featured researches published by Kei Mukai.
Journal of Pharmaceutical Sciences | 2016
Shohei Horiuchi; Naoki Choda; Haruyuki Takahashi; Tomomi Sato; Hikaru Taira; Kei Mukai
It is important to determine a critical micelle concentration (CMC) of a surfactant in a protein formulation for stabilizing the protein at maximum by preventing it from interfacial denaturation. There are several techniques for CMC determination. Among them, surface tensiometry is the most common approach because this has a long history and much data at many research fields. However, large amount of sample solution is usually required for the measurement (e.g., more than 1 mL is necessary when a standard reservoir like a glass petri dish is used). This is one of the hurdles for protein formulators because only a small amount of protein could be used at the early-stage development. In this research, we tried to minimize the required amount of sample solution for surface tension measurement by developing appropriate probe and reservoir using a three-dimensional printer (3D printer). The advantages and capabilities of 3D printer are (1) to control the shape and size of the printed material precisely, (2) to change the figure freely, and (3) to prepare the prototype quickly. After the experiments and thereby the refinement of probe as well as reservoir, we found that CMCs of polysorbate 20, polysorbate 80, and poloxamer 188 in water and protein formulations could be precisely detected using a probe 0.5 mm in diameter and small reservoir with a pocket of 7.5 mm in diameter/0.25 mm in depth which were made by a 3D printer. Furthermore, the required sample solution per each measurement could be reduced to 80 μL, which means more than 90% reduction against a standard reservoir.
Archive | 2005
Tomomichi Futo; Kei Mukai; Jiichi Arai
Archive | 2001
Kei Mukai; Muneo Nonomura; 圭 向井; 宗夫 野々村
Archive | 1999
Hisayoshi Shimizu; Muneo Nonomura; Tomomichi Futo; Kei Mukai
Archive | 2004
Hisayoshi Shimizu; Muneo Nonomura; Tomomichi Futo; Kei Mukai
Archive | 2001
Muneo Nonomura; Kei Mukai
Archive | 2016
Shohei Horiuchi; Naoki Choda; Haruyuki Takahashi; Tomomi Sato; Hikaru Taira; Kei Mukai
Archive | 2010
Hisayoshi Shimizu; Muneo Nonomura; Tomomichi Futo; Kei Mukai
Archive | 2005
Tomomichi Futo; Kei Mukai; Jiichi Arai
Archive | 1999
Hisayoshi Shimizu; Muneo Nonomura; Tomomichi Futo; Kei Mukai