Keiichi Takigawa

Investigation of nosocomial respiratory infection due to Pseudomonas cepacia by Arbitrarily primed polymerase chain reaction

We used DNA fingerprinting by the arbitrarily primed polymerase chain reaction (AP-PCR) technique for an epidemiologic investigation of Pseudomonas cepacia nosocomial isolates obtained from patients attending our hospital. This approach was compared with conventional phenotypic typing and pulsed-field gel electrophoresis (PFGE). The patterns of gel electrophoresis of the products of AP-PCR differed significantly according to differences in the concentration of Mg2+ and in pH. AP-PCR and PFGE was identical in their resolving power, as the two methods generated four different profiles and identified the same group of strains. The AP-PCR method constitutes an easy alternative to the well-established PFGE method.

A Prospective Randomized Clinical Trial Comparing Preventive and Conventional Use of rhG-CSF in Lung Cancer Patients Receiving Chemotherapy.

肺癌患者22例を対象に, 肺癌化学療法後の好中球減少症に対するG-CSFの予防的皮下投与の意義を, 現在一般的に行われている白血球減少後より使用開始する方法と比較してprospectiveに検討した. CBDCA 300mg/m2day 1, ADM 30mg/m2day1, VP-16 100mg/m2day 1-3による化学療法後, Arm Aは白血球数2,000/μl以下となった時点より, Arm Bは化学療法終了翌日であるDay4よりrh G-CSFの投与を開始した. その結果, Arm BのG-CSF予防的投与はArm Aの対症的投与に比して, 白血球数の最低値, 2,000/μl以下の期間, 4,000/μlに回復するまでの期間, 好中球数の最低値, 500/μl以下の期間を有意に改善し, 抗生物質使用日数, 発熱期間を短縮, 感染機会を減少させる傾向を認めた. G-CSFの予防的投与は化学療法の間隔を短縮し, よりdoseintensityの高い治療を行うのに有用と考えられる.