Keiji Nakagami
Teikyo University
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Publication
Featured researches published by Keiji Nakagami.
Virchows Archiv | 1987
Junji Kimura; Keiji Nakagami; Kimiko Amanuma; Shoji Ohkuma; Yoji Yoshida; Tatsuya Takano
Monoclonal antibodies against lipid-laden cells and against extracellular regions of lipid-deposits in atherosclerotic aorta were prepared. Mice were immunized with a delipidated homogenate of atherosclerotic aorta of Watanabe-heritable hyperlipidemic rabbits. Hybridomas were obtained by fusion and cultured in hypoxanthine, aminopterin and thymidine selection medium. Specific antibodies were selected by indirect immunohistochemical staining of frozen sections of atherosclerotic aorta. Nine clones that produced antibodies that stained the atherosclerotic intima exclusively were selected and cloned by limiting dilution. Finally two clones (FCR1a/201F, FCR1b/ 904B) producing antibodies specific to lipid-laden cells and one clone (EMR1a/212D) producing an antibody specific to regions with lipid deposits in the extracellular matrix were established. These monoclonal antibodies may help in understanding how lipids accumulate in atherosclerosis.
Archive | 1988
Tatsuya Takano; Chieko Mineo; Ryoichi Hashida; Y. Yagyu-Mizuno; Keiji Nakagami; Shoji Ohkuma
To elucidate the mechanism how lipoproteins transport through the endothelial barrier, an in vitro model of transcellular transport of macromolecules was developed.
Archive | 1985
Tatsuya Takano; Ryoichi Hashida; Keiji Nakagami; Junji Kimura
Yakugaku Zasshi-journal of The Pharmaceutical Society of Japan | 2008
Tatsuya Takano; Hiroyuki Itabe; Masahiro Mori; Junji Kimura; Keiji Nakagami; Ryuichiro Sato; Ryoichi Hashita; Yasuko Yagyu; Chieko Mineo; Kimiko Amanuma; Tsuneo Imanaka; Yusuke Higashi; Yasuyuki Fujimoto; Eiko Fujita
Journal of Biochemistry | 1987
Masayasu Enomoto; Keiji Nakagami; Shoji Ohkuma; Tatsuya Takano
Yakugaku Zasshi-journal of The Pharmaceutical Society of Japan | 1979
Hiroshi Hamakawa; Noriyasu Takayanagi; Keiji Nakagami; Sadami Kobari; Takeshi Shimizu
Annals of the New York Academy of Sciences | 1990
Ryuichiro Sato; Yasuomi Komine; Keiji Nakagami; Tsuneo Imanaka; Tatsuya Takano
The journal of Japan Atherosclerosis Society | 1987
Masayasu Enomoto; Keiji Nakagami; Shoji Ohkuma; Tatsuya Takano
The journal of Japan Atherosclerosis Society | 1987
Keiji Nakagami; Junji Kimura; Nobuoki Hayashi; Shoji Ohkuma; Tatsuya Takano
Archive | 1986
Tatsuya Takano; Ryoichi Hashida; Keiji Nakagami; Junji Kimura
