Keiko Murakoso

Long-term effect of 2-deoxy-2-fluoroglucose on maintenance in culture of the neonatal B cell of rat.

Abstract This report describes a beneficial role for 2-deoxy-2-fluoroglucose(2-dfg) in promoting survival of neonatal B cells of the rat. Culturing of islets in medium with 5.5 mM glucose abolished the glucose responsiveness of B cells after 7 days of culture. By contrast, the 2-dfg supplemented culture maintained glucose-induced insulin release, and the recovery of insulin in B cells at day 7 at levels 4 to 7-fold higher than at day 0. Slightly enhanced insulin biosynthesis was observed in those B cells. In addition, a selective deletion of fibroblasts was caused by the addition of the fluoroglucose.

EFFECT OF 2-DEOXY-D-GLUCOSE ON MAINTENANCE IN CULTURE OF NEONATAL B CELL OF RAT

SummaryThe effect of 2-deoxy-d-glucose on maintenance in culture of B cells of the neonatal rat was examined by supplementation of Medium 199 containing 5.5 mM glucose with 1 mM 2-deoxy-d-glucose. Islets maintained in medium with 5.5 mM glucose (basal medium) for 7 d underwent remarkable decreases in glucose sensitivity, and the levels of insulin in the medium dropped. By contrast, addition of 2-deoxy-d-glucose promoted a higher insulin content in medium and an increase in the glucose-induced insulin release and biosynthesis. Moreover, the addition of the deoxysugar caused a selective deletion of fibroblasts and prevented the deterioration of islet cells in basal medium, yielding clusters mostly consisting of islet cells at the end of culture.