Keisuke Morishima

Screening of single Escherichia coli in a microchannel system by electric field and laser tweezers

This paper proposes a novel methodology for high throughput screening of microbes. We have developed a prototype of a microchannel system for high throughput screening of Escherichia coli. Experimental demonstration of non-contact transportation and manipulation of E. coli by dielectrophoretic force and radiation pressure of laser tweezers was carried out with a Laser manipulator system. The basic strategies for improving the working efficiency and the operability of the micromanipulation are discussed, and a new direction in this field is presented. Experiments show that transportation and separation of E. coli with an electric field and optical radiation pressure is useful for future practical application to the high throughput screening of microbes.

Microflow system and transportation of DNA molecule by dielectrophoretic force utilizing the conformational transition in the higher order structure of DNA molecule

In this paper, we propose a new methodology on noncontact transportation of DNA molecules by dielectrophoretic force together with the Micro DNA Flow System. We utilize the conformational transition in the higher order structure of DNA for transportation. We designed a simple micro electrode-flow system. Experimental demonstration of DNA transportation in the globule state using dielectrophoretic force and direct observation of the DNA molecule in a non-uniform electric field were carried out with fluorescence microscopy. We discuss the experimental result on the motion of the DNA molecule. We show that transportation of DNA with the state of compacted globule is profitable in the future practical application for the separation of giant DNAs such as human gene. In future we consider the possibility of the Micro DNA Flow System as one of the biomanipulation and automation systems for DNA sequencing.

Noncontact transportation of DNA molecule by dielectrophoretic force

In this paper, we propose a new approach to noncontact transportation of DNA molecules by dielectrophoretic force and the idea of the Micro DNA Flow System. Such systems can be made by using microfabrication technology. At first, we designed a simple micro electrode-flow system and carried out experiments. Experimental demonstration of DNA transportation using dielectrophoretic force and direct observation of the DNA molecule in a non-uniform electric field was carried out with fluorescence microscopy. Motion of the DNA molecule is modeled in terms of the electric field generated by the electrode design. We compared the experimental results with the motion of the DNA molecule which is theoretically predicted. We showed a simple electrode design and the possibility of noncontact transportation of DNA molecules based on Micro DNA Flow System.

Bio-micromanipulation system for high throughput screening of microbes in microchannel

We propose a novel methodology on high throughput screening of microbes. We have developed a prototype of microchannel system for high throughput screening of Escherichia coli. Experimental demonstration of noncontact transportation and manipulation of Escherichia coli by dielectrophoretic force and radiation pressure of laser tweezers has been carried out with laser manipulator system. We discussed the basic strategies to improve the working efficiency and the operability of the micromanipulation and presented a new direction in this field. In experiments, we show that transportation and separation of Escherichia coli with electric field and optical radiation pressure is feasible in the future practical application for the high throughput screening of microbes.

Manipulation of DNA molecule utilizing the conformational transition in the higher order structure of DNA

We propose a new methodology on noncontact transportation of DNA molecules by dielectrophoretic force together with the micro DNA flow system. We utilize the conformational transition in the higher order structure of DNA for transportation. We have designed a simple micro electrode-flow system. Experimental demonstrations of DNA transportation in the globule state using dielectrophoretic force and direct observation of the DNA molecule in a nonuniform electric field were carried out with fluorescence microscopy. We discuss the experimental results on the motion of the DNA molecule. We show that transportation of DNA with the state of compacted globule is profitable in the future practical application for the separation of giant DNAs such as the human gene.

Noncontact transportation of DNA molecule by dielectrophoretic force for micro DNA flow system

In this paper, we propose a new approach to noncontact transportation of DNA molecules by dielectrophoretic force and the idea of the micro DNA flow system. Such system can be made by using microfabrication technology. At first, we designed a simple micro electrode-flow system and carried out experiments. Experimental demonstration of DNA transportation using dielectrophoretic force and direct observation of the DNA molecule in a nonuniform electric field was carried out with fluorescence microscopy. We showed a simple electrode design and the possibility of noncontact transportation of DNA molecules based on micro DNA flow system. In future we consider the possibility of the micro DNA flow system as one of the biomanipulation and automation systems for DNA sequencing.

Screening of single Escherichia coli by electric field and laser tweezer

In this paper, we propose a novel methodology for high throughput screening of microbes. We have developed a prototype of a microchannel system for high throughput screening of Escherichia coli. Experimental demonstrations of noncontact transportation and manipulation of Escherichia coli by dielectrophoretic force and radiation pressure of laser tweezers were carried out with a laser manipulator system. We discuss research issues in bio-micromanipulation and present a new direction in this field. We consider the basic strategies to improve the working efficiency and the operability of bio-micromanipulation. In experiments, we show that transportation and separation of Escherichia coli with electric field and optical radiation pressure is profitable for future practical application in high throughput screening of microbes.

Transportation of DNA molecule by dielectrophoretic force utilizing the conformational transition in the higher order structure of DNA

We propose a new methodology on noncontact transportation of DNA molecules by a dielectrophoretic force together with the micro DNA flow system. We utilize the conformational transition in the higher order structure of DNA for transportation. We designed a simple micro electrode flow system. Experimental demonstration of DNA transportation in the globule state using a dielectrophoretic force and direct observation of the DNA molecule in a non-uniform electric field were carried out with fluorescence microscopy. We discuss the experimental results on the motion of the DNA molecule. We show that transportation of DNA with the state of compacted globule is profitable in the future practical application for the separation of giant DNAs such as the human gene.

Micromanipulation system for handling of biological molecule and screening of microbes in a microchannel by electric field and laser tweezers

In this paper, we propose a novel methodology on noncontact transportation of DNA molecules by dielectrophoretic force and high throughput screening of microbes. First, we utilize the conformational transition in the higher order structure of DNA for transportation. We designed a simple micro electrode-flow system. Experimental demonstration of DNA transportation in the globule state using dielectrophoretic force and direct observation of the DNA molecule in a non- uniform electric field were carried out with fluorescence microscopy. We discuss the experimental results on the motion of the DNA molecule. We show that transportation of DNA with the state of compacted globule is profitable in the future practical application for the separation of giant DNAs such as human gene. Next, we have developed a prototype of Microchannel system for high throughput screening of Escherichia coli. Experimental demonstration of noncontact transportation and manipulation of Escherichia coli by dielectrophoretic force and radiation pressure of laser tweezers were carried out with laser manipulator system. We discussed the basic strategies to improve the working efficiency and the operability of the micromanipulation and presented a new direction in this field. In experiments, we show that transportation and separation of E. coli cells by dielectrophoretic force and optical trapping is useful for future practical application to the high throughput screening of microbes. We showed the possibility of the Microchannel system as one of the biomanipulation and automation systems for DNA sequencing and pharmaceutical field.