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Dive into the research topics where Keisuke Nakajima is active.

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Featured researches published by Keisuke Nakajima.


Journal of Biological Chemistry | 1997

Induction of Apoptosis and CPP32 Expression by Thyroid Hormone in a Myoblastic Cell Line Derived from Tadpole Tail

Yoshio Yaoita; Keisuke Nakajima

During amphibian metamorphosis, the tail and gills that are useful in aquatic life but inappropriate for terrestrial activity are induced to degenerate completely in several days by endogenous thyroid hormone (TH). The dramatic resorption of the tadpole tail has attracted a good deal of attention as an experimental system of cell death, but the mechanism has not been well characterized. To facilitate in vitro analysis, we have established a myoblast cell line (XLT-15) derived from the Xenopus laevis tadpole tail. This cultured cell line died in response to TH and exhibited positive TUNEL reaction and internucleosomal DNA cleavage. Simultaneously, expression of the Xenopus CPP32/apopain/Yama gene was up-regulated by TH in the cell line as it is in regressing tadpole tail, whereas interleukin-1β-converting enzyme (ICE) mRNA is around 1 copy/cell in tail and undetectable in XLT-15 cells. A CPP32/apopain/Yama inhibitor (acetyl-Asp-Glu-Val-Asp-aldehyde) prevented TH-induced apoptosis of XLT-15 cells, but an ICE inhibitor (acetyl-Tyr-Val-Ala-Asp-aldehyde) did not. These results suggested that an increase of CPP32/apopain/Yama gene expression is involved in TH-dependent apoptosis of XLT-15 and tadpole tail resorption during metamorphosis.


Collagen and related research | 1988

Quantitative Evaluation of the Factors Affecting the Process of Fibroblast-Mediated Collagen Gel Contraction by Separating the Process into Three Phases

Toshio Nishiyama; Naoki Tominaga; Keisuke Nakajima; Toshihiko Hayashi

Kinetics of collagen gel contraction by fibroblasts cultured in vitro was examined in detail for quantitative analysis. The process of collagen gel contraction was not expressed by a simple function of time. It appeared to consist of three distinct phases; a lag phase before the initiation of contraction, a rapid contraction phase and a slow contraction phase. Factors affecting the gel contraction can be classified into four groups. The first group includes increase in cell number, in culture temperature or in serum concentration, which strengthened the contraction in all the three phases, suggesting that they affected cellular activity particularly in interacting with collagen. The second group repressed the later two phases of contraction but not the first lag phase, typically increase in collagen concentration and a low dose of nocodazole or colcemid. Increasing population doubling levels of fibroblasts belongs to the third group which caused a reduced lag time but no change in the later two phases. Cytochalasin D at a low dose (0.03-0.1 microgram/ml) is another example of the third group which shortened the lag time. The last group did not change the contraction curves. Donor age of fibroblasts isolated from the skin is an example of this group. The rate of rapid contraction in the second phase was always found to be closely correlated with the degree of contraction at the end of the third phase, in a whole set of the factors above mentioned. The results suggest that the extent of the later two phases might be a reflection of the same cellular activity, particularly cytokinetical one. The lag time is directly related to the time for cells to become elongate in shape as observed by using the video-microscopy, suggesting that the lag phase is also governed by cytokinetical activity. The two cytokinetical activities are closely related, but may be distinct, since the factors affecting the collagen gel contraction can be differentiated into four groups.


Developmental Dynamics | 2003

Dual mechanisms governing muscle cell death in tadpole tail during amphibian metamorphosis

Keisuke Nakajima; Yoshio Yaoita

The tadpole tail, which is twice as long as the body, is induced to resorb completely by thyroid hormone within several days during the anuran metamorphosis. To investigate the underlying mechanism, we undertook two approaches. First, we examined the effect of dominant‐negative thyroid hormone receptor (DNTR) on muscle cell death in vitro. The overexpression of DNTR suppressed the death of a tail‐derived myoblastic cell line induced by thyroid hormone. Second, tadpole tails were injected with a reporter gene and the DNTR expression construct, and the reporter gene expression in muscle cells was followed during the spontaneous metamorphosis. DNTR overexpression inhibited a decrease of the reporter gene expression that began at stage 57 in the control tadpoles but only delayed massive muscle cell death at stage 63 when tails shrink very rapidly. Some remained even a few weeks after the metamorphosis, although most DNTR‐overexpressing cells died by the end of the metamorphosis. These results led us to propose that thyroid hormone induces the suicide of muscle cells (the cell‐autonomous death) in the tail between stage 57 and 62 and that both the murder and suicide mechanisms execute muscle cell death in stage 62–64 to remove muscle promptly and completely. Developmental Dynamics 227:246–255, 2003.


Development Growth & Differentiation | 2012

Generation of albino Xenopus tropicalis using zinc-finger nucleases

Keisuke Nakajima; Taeko Nakajima; Minoru Takase; Yoshio Yaoita

To generate albino lines of Xenopus tropicalis, we injected fertilized eggs with mRNAs encoding zinc‐finger nucleases (ZFNs) targeting the tyrosinase coding region. Surprisingly, vitiligo was observed on the skin of F0 frogs that had been injected with ZFN mRNAs, indicating that both tyrosinase genes in the genome were disrupted in all melanocytes within the vitiligo patches. Mutation analysis using genomic DNA from the skin revealed that two mosaic F0 frogs underwent spatially complex tyrosinase gene mutations. The data implies that the ZFN‐induced tyrosinase gene ablations occurred randomly over space and time throughout the entire body, possibly until the young tadpole stage, and that melanocyte precursors lacking functional tyrosinase proliferated and formed vitiligo patches. Several albino X. tropicalis, which are compound heterozygotes for biallelic tyrosinase mutations, were obtained by mating the mosaic F0 frogs. To our knowledge, this is the first report of the albino vertebrates generated by the targeted gene knockout.


Development Growth & Differentiation | 2007

Expression of matrix metalloproteinase genes in regressing or remodeling organs during amphibian metamorphosis

Kenta Fujimoto; Keisuke Nakajima; Yoshio Yaoita

Several matrix metalloproteinases (MMP) are induced by thyroid hormone (TH) during the climax of amphibian metamorphosis and play a pivotal role in the remodeling of the intestine and the regressing tail and gills by degrading the extracellular matrix (ECM). We compared MMP gene expression levels precisely by quantitative real‐time reverse transcription‐polymerase chain reaction. The expression of MMP genes increases prominently at Nieuwkoop and Faber (NF) stages 60, 60–61 and 62 in the intestine, gills and tail, respectively, when the drastic morphological changes start in each organ. Gene expression analysis in the TH‐treated tadpoles and cell line revealed that MMP mRNAs are upregulated in response to TH quickly within several hours to low levels and then increase in a day to high levels. All TH‐induced MMP genes have TH response elements (TREs). The presence of high affinity TREs in MMP genes correlates with early TH‐induction. Based on these results, we propose that TH stimulates the transcription of MMP genes through TREs within several hours to low levels and then brings about the main increase of mRNAs by TH‐induced transcriptional factors, including TH receptor β, in a cell type‐specific transcriptional environment.


Development Growth & Differentiation | 2006

One of the duplicated matrix metalloproteinase-9 genes is expressed in regressing tail during anuran metamorphosis

Kenta Fujimoto; Keisuke Nakajima; Yoshio Yaoita

The drastic morphological changes of the tadpole are induced during the climax of anuran metamorphosis, when the concentration of endogenous thyroid hormone is maximal. The tadpole tail, which is twice as long as the body, shortens rapidly and disappears completely in several days. We isolated a cDNA clone, designated as Xl MMP‐9TH, similar to the previously reported Xenopus laevis MMP‐9 gene, and showed that their Xenopus tropicalis counterparts are located tandemly about 9 kb apart from each other in the genome. The Xenopus MMP‐9TH gene was expressed in the regressing tail and gills and the remodeling intestine and central nervous system, and induced in thyroid hormone‐treated tail‐derived myoblastic cultured cells, while MMP‐9 mRNA was detected in embryos. Three thyroid hormone response elements in the distal promoter and the first intron were involved in the upregulation of the Xl MMP‐9TH gene by thyroid hormone in transient expression assays, and their relative positions are conserved between X. laevis and X. tropicalis promoters. These data strongly suggest that the MMP‐9 gene was duplicated, and differentiated into two genes, one of which was specialized in a common ancestor of X. laevis and X. tropicalis to be expressed in degenerating and remodeling organs as a response to thyroid hormone during metamorphosis.


Biology Open | 2013

Comparison of TALEN scaffolds in Xenopus tropicalis

Keisuke Nakajima; Yoshio Yaoita

Summary Transcription activator-like effector nucleases (TALENs) are facile and potent tools used to modify a gene of interest for targeted gene knockout. TALENs consist of an N-terminal domain, a DNA-binding domain, and a C-terminal domain, which are derived from a transcription activator-like effector, and the non-specific nuclease domain of FokI. Using Xenopus tropicalis (X. tropicalis), we compared the toxicities and somatic mutation activities of four TALEN architectures in a side-by-side manner: a basic TALEN, a scaffold with the same truncated N- and C-terminal domains as GoldyTALEN, a scaffold with the truncated N- and C-terminal domains and an obligate heterodimeric nuclease domain, and a scaffold with the truncated N- and C-terminal domains and an obligate heterodimeric Sharkey nuclease domain. The strongest phenotype and targeted somatic gene mutation were induced by the injection of TALEN mRNAs containing the truncated N- and C-terminal domains and an obligate heterodimeric nuclease domain. The obligate heterodimeric TALENs exhibited reduced toxicity compared to the homodimeric TALENs, and the homodimeric GoldyTALEN-type scaffold showed both a high activity of somatic gene modification and high toxicity. The Sharkey mutation in the heterodimeric nuclease domain reduced the TALEN-mediated somatic mutagenesis.


Genes to Cells | 2012

Regulation of thyroid hormone sensitivity by differential expression of the thyroid hormone receptor during Xenopus metamorphosis.

Keisuke Nakajima; Kenta Fujimoto; Yoshio Yaoita

During amphibian metamorphosis, a series of dynamic changes occur in a predetermined order. Hind limb morphogenesis begins in response to low levels of thyroid hormone (TH) in early prometamorphosis, but tail muscle cell death is delayed until climax, when TH levels are high. It takes about 20 days for tadpoles to grow from early prometamorphosis to climax. To study the molecular basis of the timing of tissue‐specific transformations, we introduced thyroid hormone receptor (TR) expression constructs into tail muscle cells of Xenopus tadpoles. The TR‐transfected tail muscle cells died upon exposure to a low level of thyroxine (T4). This cell death was suggested to be mediated by type 2 iodothyronine deiodinase (D2) that converts T4 to T3—the more active form of TH. D2 mRNA was induced in the TR‐overexpressing cells by low levels of TH. D2 promoter contains a TH‐response element (TRE) with a lower affinity for TR. These results show that the TR transfection confers the ability to respond to physiological concentrations of TH at early prometamorphosis to tail muscle cells through D2 activity and promotes TH signaling. We propose the positive feedback loop model to amplify the cells ability to respond to low levels of T4.


Developmental Biology | 2015

Xenopus pax6 mutants affect eye development and other organ systems, and have phenotypic similarities to human aniridia patients.

Takuya Nakayama; Marilyn Fisher; Keisuke Nakajima; Akinleye O. Odeleye; Keith Zimmerman; Margaret B. Fish; Yoshio Yaoita; Jena L. Chojnowski; James D. Lauderdale; Peter A. Netland; Robert M. Grainger

Mutations in the Pax6 gene cause ocular defects in both vertebrate and invertebrate animal species, and the disease aniridia in humans. Despite extensive experimentation on this gene in multiple species, including humans, we still do not understand the earliest effects on development mediated by this gene. This prompted us to develop pax6 mutant lines in Xenopus tropicalis taking advantage of the utility of the Xenopus system for examining early development and in addition to establish a model for studying the human disease aniridia in an accessible lower vertebrate. We have generated mutants in pax6 by using Transcription Activator-Like Effector Nuclease (TALEN) constructs for gene editing in X. tropicalis. Embryos with putative null mutations show severe eye abnormalities and changes in brain development, as assessed by changes in morphology and gene expression. One gene that we found is downregulated very early in development in these pax6 mutants is myc, a gene involved in pluripotency and progenitor cell maintenance and likely a mediator of some key pax6 functions in the embryo. Changes in gene expression in the developing brain and pancreas reflect other important functions of pax6 during development. In mutations with partial loss of pax6 function eye development is initially relatively normal but froglets show an underdeveloped iris, similar to the classic phenotype (aniridia) seen in human patients with PAX6 mutations. Other eye abnormalities observed in these froglets, including cataracts and corneal defects, are also common in human aniridia. The frog model thus allows us to examine the earliest deficits in eye formation as a result of pax6 lesions, and provides a useful model for understanding the developmental basis for the aniridia phenotype seen in humans.


Zoological Science | 2013

Targeted Gene Disruption in the Xenopus tropicalis Genome using Designed TALE Nucleases

Keisuke Nakajima; Yuya Nakai; Morihiro Okada; Yoshio Yaoita

Transcription activator-like effector nucleases (TALENs) are attractive and powerful molecular tools for targeted gene disruption because of their simple design and quick assembly. To evaluate the utility of TALENs in genome editing in Xenopus tropicalis, we prepared nine pairs of TALENs for the tyrosinase, noggin and MMP-9TH genes. All of the TALENs had some activity in a single-strand annealing assay using a cultured frog cell line, suggesting double-stranded DNA cleavage activity by the TALENs at their target site. The injection of mRNAs encoding TALENs into fertilized X. tropicalis embryos resulted in Cel-1 cleavage of the PCR fragment containing the target site amplified from embryo genomic DNA, indicating that a mutation in the target gene had occurred during embryogenesis. These mutations were confirmed by the sequencing of clones derived from the PCR fragments of genomic DNA. Patches of vitiligo were observed in tadpoles raised from fertilized eggs that had been injected with mRNAs of TALENs for the tyrosinase gene. TALENs containing the repeat variable di-residue (RVD) NN appeared to show more activity than TALENs containing RVD NK, although both RVD NN and NK preferentially associate with a G nucleotide.

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