Keita Soda

Laboratory-size three-dimensional x-ray microscope with Wolter type I mirror optics and an electron-impact water window x-ray source

We constructed a laboratory-size three-dimensional water window x-ray microscope that combines wide-field transmission x-ray microscopy with tomographic reconstruction techniques, and observed bio-medical samples to evaluate its applicability to life science research fields. It consists of a condenser and an objective grazing incidence Wolter type I mirror, an electron-impact type oxygen Kα x-ray source, and a back-illuminated CCD for x-ray imaging. A spatial resolution limit of around 1.0 line pairs per micrometer was obtained for two-dimensional transmission images, and 1-μm scale three-dimensional fine structures were resolved.

Determination of the temperature of bremsstrahlung photon generated by ultraintense laser using various thickness attenuators

We evaluate the simplified method using the Lambert-Beer law to measure the temperature of bremsstrahlung photon generated by an ultraintense laser. Analytical values are compared to the results of the Monte Carlo calculation of GEANT4 and they agreed very well on the condition of the appropriate distance between the attenuator and the detector. We performed the experiment to measure the temperature of bremsstrahlung x-ray emitted from a metal target irradiated by a Ti:sapphire laser with 76 mJ, 72 fs, 2.2 × 10(18)  W∕cm(2). For a Cu target of 30  μm thick, the photon temperature was reasonably determined to be 0.18 MeV, which is in good agreement with previous studies.

Laboratory-size three-dimensional water-window x-ray microscope with Wolter type I mirror optics

We constructed a laboratory-size three-dimensional water-window x-ray microscope that combines wide-field transmission x-ray microscopy with tomographic reconstruction techniques. It consists of an electron-impact x-ray source emitting oxygen Kα x-rays, Wolter type I grazing incidence mirror optics, and a back-illuminated CCD for x-ray imaging. A spatial resolution limit better than 1.0 line pairs per micrometer was obtained for two-dimensional transmission images, and 1-μm-scale three-dimensional fine structures were resolved.

Plasma irradiation effects in the abdominal adhesion mouse model

Plasma phenomenon has been demonstrated as a new promising tool in medical field, although little is known about the plasma irradiation effects on tissues in abdominal cavity. Therefore finding the possible marker is the first and the key step to evaluate the events after plasma irradiation and a suitable mouse model is required to validate the plasma effects. We focused on abdominal adhesion formation that is the secondary event after surgery irrespective of the any surgical devices, and is associated with complications such as ileus, abdominal pain or infertility. Firstly we generated a mouse abdominal adhesion model using bipolar forceps. Within 7days, cauterized cecum was demonstrated to recruit the epididymal adipose tissue (EAT), which connected tissues abnormally and infiltrated inflammatory cells into EAT were observed by microscopic examination. We also identified some inflammatory proteins released into peritoneal cavity by proteomics of ascites after cauterization. Moreover, we measured serum cytokines level and determined that adhesion could be evaluated as a result of the alteration of pro-inflammatory and anti-inflammatory cytokines. Now we are pursuing to assess the plasma irradiation effects using those inflammatory proteins and cytokines as an appropriate marker.