Kelen Christine do Nascimento Souza

Assessment of angiogenesis by CD105 antigen in epithelial salivary gland neoplasms with diverse metastatic behavior

BackgroundInformation on the biology of metastasis development in salivary gland tumors is scarce. Since angiogenesis seems associated with this phenomenon in other tumors, we sought to compare salivary gland tumors with diverse metastatic behavior in order to improve the knowledge and management of these lesions.MethodsSamples from the most important salivary gland tumors were segregated according to its metastatic behavior and submitted to routine immunohistochemistry to identify vessels positive for CD105 expression. Frequency of positive cases and intratumoral microvessel density (IMD) was compared among the group of lesions.ResultsCD105 positive vessels were absent in normal salivary gland tissue, were rare in pleomorphic adenomas and adenoid cystic carcinomas (ACC), more common in polymorphous low-grade adenocarcinomas and highest in mucoepidermoid carcinomas. Only ACC with such feature were metastatic. IMD was higher in malignant rather than benign tumors.ConclusionImmunostaining of CD105 in salivary gland tumors implies participation of angiogenesis in the development of malignant lesions, as well as some role for myoepithelial cells in the control of new vessel formation. In addition, suggest that ACC with positive CD105 vessels are at higher risk for metastasis.

Giant Leiomyomatous Hamartoma of the Tongue

t U f r b u s i o p amartoma has been defined as a focal benign tumorike mass composed of a disordered mixture of maure tissues that normally occur in the affected part, ut with predominance of one particular tissue to this rea. Clinically, these lesions grow in concert with he patient and therefore do not show any tendency or uncontrolled growth. In oral tissues, hamartomas omposed of blood or lymphatic vessels (hemangioas and lymphangiomas) are relatively common. hese are usually single lesions in otherwise healthy atients, while there are eventual reports of associaion with congenital developmental defects such as

Cervical Castleman's Disease in Childhood

Castleman’s disease (CD) is an uncommon benign non-neoplastic lymphoproliferative disease. It was first described by Castleman and colleagues in 2 different papers, which described progressive enlargement of the mediastinal lymph nodes. 1,2 The etiology and pathogenesis of CD remains to be established. Different hypotheses, such as chronic inflammatory process associated or not to viral infection, angiolymphoid hamartomatous or choristomatous process, immunologic disturbance, and neoplastic transformation have been raised to explain it. 3,4 Recently, a unifying model of pathogenesis has implicated human herpes virus-8-stimulated interleukin-6 production by B-cell mantle zone. In turn, IL-6 would induce vascular endothelial growth factor production and B-cell proliferation, resulting in an increased vascularization and cellularity in the affected lymph nodes. 4

Vascular endothelial growth factor and thymidine phosphorylase expression in salivary gland tumors with distinct metastatic behavior

BACKGROUND Metastasis of salivary gland tumors has a negative impact on survival. Angiogenesis and its factors are potential markers for predicting metastasis in different malignant tumors, but this is not the case for salivary gland tumors. METHODS Salivary gland tumors of distinct biologic behavior were analyzed according to the semiquantitative immunoexpression of vascular endothelial growth factor (VEGF) and thymidine phosphorylase (TP). RESULTS Vascular endothelial growth factor expression was predominantly weak in benign tumors. Weak TP expression was observed in 100% cases of benign tumors and in 74.3% of primary malignant tumors. High VEGF and TP expression levels were significantly associated with primary malignant tumors but not with primary non-metastasizing and primary metastasizing malignant tumors or with subtypes of malignant tumors. CONCLUSIONS Vascular endothelial growth factor and TP expression levels discriminate benign and malignant tumors but cannot predict metastasis from non-metastasizing tumors.

Multicentric granular cell tumor: report of a patient with oral and cutaneous lesions

Granular cell tumor (GCT) is an uncommon benign neoplasm of soft tissue that characteristically affects the oral cavity, with increased frequency in the tongue. In this paper, we report a multicentric GCT affecting a 41-year-old black woman, with nodules in perineum, groin, vulva, tongue and lip. Some of these lesions were surgically resected, but in a six-year follow-up, new primary lesions and recurrences were observed. In addition, the patient opted to do not remove some of these lesions, but there were not significant intercurrences. In view of the present findings, aspects related to clinical presentation, multiplicity, malignant potential, and treatment of GCT are discussed.

Warthin's tumor at the Brazilian National Cancer Institute: additional evidence of homogeneous sex prevalence and association with other neoplasms.

Purpose of the Study: To report the largest Brazilian series of Warthin’s tumor (WT). Procedures: The medical files of 76 patients with WT treated in the Brazilian National Cancer Institute from 1996 to 2006 were reviewed. Results: The male:female ratio was 2:1, with a predominance of white, old, and smoking patients. However, there were more women among the nonsmokers. One fifth of the patients presented with synchronous multiple lesions. Parotid lesions were prevalent, but there were patients with lesions in cervical lymph nodes and in the inferior lip. Most cases were treated by superficial parotidectomy, without recurrences. One fourth of the patients also developed other primary neoplasms. Conclusions: The observed data do not differ from those in the international literature. Multiplicity and the development of other neoplastic diseases require close clinical management of patients with WT. Message: The previously unreported observation of female prevalence among nonsmoking people with WT should be confirmed and explored in future studies.

Myoepithelial carcinoma of the submandibular gland: report of a case with multiple cutaneous metastases

Myoepithelial carcinoma is a rare and aggressive neoplasm of the salivary glands. One-third of the patients may develop regional distant metastases, and lungs and kidneys have been regarded as the most usual sites for implantation. There is, however, little information on the metastatic behavior of this malignancy. We report the first case of patient with multiple cutaneous metastases from a myoepithelial carcinoma of the submandibular gland, which depicted a very aggressive clinical course.

Expressão imuno-histoquímica de p53 na discriminação do comportamento biológico dos tumores de glândulas salivares

BACKGROUND: Mutations in the tumor suppressor gene TP53 have been found in many tumors, including salivary gland neoplasms (SGN). However, there is not consensus about the influence of p53 protein expression on the biological behavior of SGN. OBJECTIVES: To compare the expression of p53 between benign and malignant neoplasms, among histologic subtypes of those tumors, and among (1) tumors that did not metastasize; (2) primary lesions that emitted metastasis; and (3) metastasis of SGN, by immunohistochemistry. MATERIAL AND METHOD: It was evaluated 16 cases of pleomorphic adenoma (PA), 17 samples of primary malignant tumors that did not metastasize throughout preservation (seven adenoid cystic carcinoma [ACC] and ten mucoepidermoid carcinomas [MC]), 13 malignant neoplasms that emitted metastasis (nine ACC and four MC) and 12 regional or distant metastasis (five ACC and seven MC) by immunohistochemical detection of p53 antigen (streptavidin-biotin-peroxidase method). Statistical analyses were performed by Mann-Whitney and Kruskal-Wallis tests. p value below 0.05 was considered significant. RESULTS: A significant difference between benign and malignant lesions (p = 0.03) and among different histological types (p = 0.02) was observed, notably between PA and MC. Malignant lesions with distinct biological behavior did not differ according to this analysis.

Estudo da expressão de galectina-3 em carcinomas de língua de camundongos

BACKGROUND: Galectin-3 (Gal-3) is a b-galactoside-binding mammalian lectin. Numerous studies have demonstrated that Gal-3 plays an important role in tumor biology, acting in some events such as apoptosis, metastasis and malignant transformation. However, in carcinomas of head and neck, the real significance of Gal-3 expression requires a better understanding. OBJECTIVES: The aim of this paper was to evaluate Gal-3 expression in tongue carcinomas experimentally induced in mice challenged with carcinogen 4-nitroquinoline-1-oxide (4NQO). MATERIAL AND METHOD: Forty-two C57BL/6 male mice were challenged with 4NQO in drinking water for 16 weeks and killed at different periods after induction. In each period, their tongues were removed, routinely processed, stained with hematoxylin and eosin (H&E) and microscopically analyzed as to the presence of carcinoma. Immunohistochemical test for Gal-3 and a descriptive analysis of its expression in induced tumors were performed. RESULTS: By the end of the experiment, 15 tumors had been induced. In the non-neoplastic lingual epithelium, strong and weak immunoreactivity for Gal-3 was noted in parabasal and superficial layers, respectively. In the basal layer, Gal-3 expression varied from absent to weak. All the well-differentiated carcinomas showed weak immunoreactivity for Gal-3, except in keratinized areas. The only case of poorly differentiated squamous cell carcinoma indicated strong immunoreactivity for Gal-3. CONCLUSION: Our results show that malignant transformation is associated with reduced expression of Gal-3, whereas its increased expression in poorly differentiated carcinoma seems to be connected with tumor aggressiveness.