Kellen Lagares Ferreira Silva

CARACTERIZAÇÃO ANATÔMICA E HISTOQUÍMICA DE RAÍZES E FOLHAS DE PLÂNTULAS DE Anacardium occidentale L. (Anacardiaceae)

Anacardium occidentale L. (Anacardiaceae) is a typical species of the Central Brazil cerrado and has socioeconomic importance. The present study aimed to characterize anatomically and histochemically the roots and leaves of this species at different stages of early development. For anatomical characterization, the samples were fixed in FAA50, embedded in paraffin and subjected to the usual procedures for light microscopy. Histochemical tests were performed to detect total lipids, tannins, lignin, starch and terpenoids with a carbonyl group in the leaves, and test for starch in roots in fresh cuts. The root has uniseriate epidermis, phloem and xylem with secretory ducts tetrarc. Five days after germination the plant presents regions with secondary growth and has the pith in the root, with storage function of starch grains. The leaves exhibit uniseriate epidermis with glandular trichomes, thick cuticle and paracytic stomata on both sides. The mesophyll is dorsiventral and midrib presents collateral vascular bundles with secretory ducts associated with phloem. The histochemical characterization showed lipids and phenolic compounds, including tannins and lignin in different tissues of the leaf. The seedling has characteristics that demonstrate its adaptation to Cerrado, as thick cuticle, dorsiventral mesophyll, secondary growth, presence of trichomes, even when cultivated in greenhouse.

Caracterização anatômica e micromorfométrica em folhas de Caryocar brasiliense

The current study aims to study the anatomy and micromorphometry of C. brasiliense leaves in three Cerrado (savanna) vegetation-types: dense, typical and sparse) in the municipality of Porto Nacional-TO. Samples were collected, fixed and stored in alcohol 70%. Transverse, longitudinal and paradermal sections of the median leaflet were prepared for anatomical and micromorphometric studies using standard techniques. C. brasiliense leaflets show uniestratified epidermis covered by thick cuticle on the adaxial surface and by paracytic stomata and multicellular non-glandular trichomes on the abaxial surface. The mesophyll is formed by two or three layers of palisade parenchyma adaxially and spongy parenchyma abaxially. The vascular bundle is of the collateral type and the accessory bundles show a sheath extending into the epidermides. The micromorphometric analyses pointed to significant differences in the thickness of both adaxial epidermis and spongy parenchyma in all specimens from the three vegetation-types. The highest averages were found in the leaflets of sparse cerrado plants, suggesting that environmental factors may have an influence over the plants morphological responses.current study aims to study the anatomy and micromorphometry of C. brasiliense leaves in three Cerrado (savanna) vegetation-types: dense, typical and sparse) - in the municipality of Porto Nacional-TO. Samples were collected, fixed and stored in alcohol 70%. Transverse, longitudinal and paradermal sections of the median leaflet were prepared for anatomical and micromorphometric studies using standard techniques. C. brasiliense leaflets show uniestratified epidermis covered by thick cuticle on the adaxial surface and by paracytic stomata and multicellular non-glandular trichomes on the abaxial surface. The mesophyll is formed by two or three layers of palisade parenchyma adaxially and spongy parenchyma abaxially. The vascular bundle is of the collateral type and the accessory bundles show a sheath extending into the epidermides. The micromorphometric analyses pointed to significant differences in the thickness of both adaxial epidermis and spongy parenchyma in all specimens from the three vegetation-types. The highest averages were found in the leaflets of sparse cerrado plants, suggesting that environmental factors may have an influence over the plants morphological responses.