Ken-ichi Fukuhara

Rapid confirmation and revision of the primary structure of bovine serum albumin by ESIMS and frit-FAB LC/MS

Incorrectness of the amino acid sequence of bovine serum albumin (BSA) was suggested from the observed molecular weight of BSA obtained by electrospray ionization mass spectrometry (ESIMS). Lack of a tyrosine residue in the position of 156th was found rapidly, by the combination of frit-fast atom bombardment mass spectrometry/liquid chromatography (Frit-FAB LC/MS), automated Edman degradation and tandem mass spectrometry (MS). Then it turned out that BSA is composed of 583 amino acid residues, and that its average molecular weight is not 66267.1, and it is corrected to 66430.3. Moreover the amino acid sequence of the positions of 94th and 95th was corrected to -QE- by using automated Edman degradation method.

Purification and properties of a pepstatin-insensitive carboxyl proteinase from a Gram-negative bacterium

A carboxyl proteinase was found in the culture filtrate of a Gram-negative bacterium. The optimum for the action of the purified enzyme was approx. pH 3 and its caseinolytic activity was not inhibited by carboxyl proteinase inhibitors, such as pepstatin, Streptomyces pepsin inhibitor and diazoacetyl-DL-norleucine methyl ester. 1,2-epoxy-3-(p-nitrophenoxy)propane modified the enzyme with concomitant loss of its enzyme activity. The enzymatic and physicochemical properties of the enzyme were compared with those of known pepstatin- and diazoacetyl-DL-norleucine methyl ester-insensitive carboxyl proteinases previously reported. To our knowledge, this is the first carboxyl proteinase isolated from bacteria.

Structural elucidation of talopeptin (MK-I), a novel metallo proteinase inhibitor produced by streptomyces mozunensis MK-23

Abstract The structure of talopeptin (MK-I), a novel metallo proteinase inhibitor produces by Streptomyces mozunensis MK-23 was elucidated to be 6-deoxy-α- l -talopyranosyloxyphospho- l -leucyl- l -tryptophan.

Tertiary structural models for human interleukin‐6 and evaluation by a sequence‐structure compatibility method and NMR experimental information

© 1997 Federation of European Biochemical Societies.