Kenan Karagöz

Plant growth-promoting rhizobacteria improved growth, nutrient, and hormone content of cabbage (Brassica oleracea) seedlings

Metin TURAN, Melek EKİNCİ, Ertan YILDIRIM*, Adem GÜNEŞ, Kenan KARAGÖZ, Recep KOTAN, Atilla DURSUN Department of Genetics and Bioengineering, Faculty of Engineering and Architecture, Yeditepe University, Kayışdağı, İstanbul, Turkey Department of Horticulture, Faculty of Agriculture, Atatürk University, Erzurum, Turkey Department of Soil Science, Faculty of Agriculture, Erciyes University, Kayseri, Turkey Department of Plant Protection, Faculty of Agriculture, Atatürk University, Erzurum, Turkey

INOCULATION EFFECTS OF PANTOEA AGGLOMERANS STRAINS ON GROWTH AND CHEMICAL COMPOSITION OF PLUM

In this study, five non-pathogenic Pantoea agglomerans strains were utilized. The objective of this study was to test for biochemical characteristics of these strains, and to evaluate their inoculation effects on fruit set rate, fruit pomological traits, fruit chemical compositions and some vegetative parameters of plum cultivar ‘Stanley’. The results showed that some of the tested strains had beneficial effects on the fruit set rate, fruit pomological traits, fruit chemical composition and/or some vegetative growth parameters of plum in comparison to the control. In conclusion, tested Pantoea agglomerans strains (especially RK-85) are the suitable inoculants for plum cultivation in cold areas such as Erzurum, and these strains may be considered as biofertilizer and protector sensitive plants against frost damage by applying in suitable timing and dose.

Effect of essential oil of Origanum rotundifolium on some plant pathogenic bacteria, seed germination and plant growth of tomato

The aim of this study is to determine effect of Origanum rotundifolium’s essential oil on some plant pathogenic bacterias, seed germination and plant growth of tomato. Xanthomonas axanopodis pv. vesicatoria strain (Xcv-761) and Clavibacter michiganensis ssp. michiganensis strain (Cmm) inoculated to tomato seed. The seeds were tested for germination in vitro and disease severity and some plant growth parameters in vivo. In vitro assay, maximum seed germination was observed at 62,5 µl/ml essential oil treatment in seeds inoculated with Xcv-761 and at 62,5 µl/ml essential oil and streptomycin treatment in seeds inoculated with Cmm. The least infected cotiledon number was observed at 500 µg/ml streptomycin treatment in seeds inoculated with Cmm. In vivo assay, maximum seed germination was observed at 250 µl/ml essential oil teratment in tomato inoculated with Cmm. Lowest disease severity, is seen in the CMM infected seeds with 250 µl/ml essential oil application these results were statistically significant wh...

Identification and characterisation of potential biofertilizer bacterial strains

In this study we aimed that isolation, identification and characterizations of PGPR strains from rhizosphere of legume plants. 188 bacterial strains isolated from different legume plants like clover, sainfoin and vetch in Erzurum province of Turkey. These three plants are cultivated commonly in the Erzurum province. It was screen that 50 out of 188 strains can fix nitrogen and solubilize phosphate. These strains were identified via MIS (Microbial identification system). According to MIS identification results, 40 out of 50 strains were identified as Bacillus, 5 as Pseudomonas, 3 as Paenibacillus, 1 as Acinetobacter, 1 as Brevibacterium. According to classical test results, while the catalase test result of all isolates are positive, oxidase, KOH and starch hydrolysis rest results are variable.

Erwinia amylovora Strainlerinin Streptomisin Sülfata Karşı Duyarlılıkları ve Strainlerin Yağ Asidi Metil Esterleri ile Antibiyotiğe Duyarlılıkları Arasındaki İlişkinin Belirlenmesi / Determination of Relationship between Streptomycin Sulphate Susceptibili

OZET: Bu calismada; Erzurum, Erzincan, Artvin, Kars ve Igdir illerinde yumusak cekirdekli meyve agaclarindan izole edilen ates yanikligi hastaliginin etmeni Erwinia amylovora’nin toplam 41 straininin streptomisin sulfata karsi duyarliliklari test edilmis; mikrobial tani sistemi (MIS) ile strainlerin yag asidi metil ester (FAME) gruplari belirlenerek, patojenin yag asitleri ve antibiyotiklere duyarliliklari arasindaki iliski arastirilmistir. Elde edilen sonuclara gore strainlerin % 31,7’sinde dayaniklilik tespit edilmistir. Yag asidi metil esterleri bakimindan patojenin 4 farkli grubunun oldugu belirlenmistir. Gruplar arasindaki farkliligin ise % 8 ile % 24 arasinda degistigi gorulmustur. Ayrica dayaniklilik oraninin 2. grupta en yuksek duzeyde (% 83.3) oldugu tespit edilmistir. Bu oran 1. grup icin % 27.6 bulunurken 3. ve 4. grupta dayanikli straine rastlanmistir. Ikinci grubun farki; 18:1 w7c yag asidinin diger gruplara gore cok yuksek duzeyde olmasi (% 30.96-43.8) ve diger gruplarin hic birisinde bulunmayan 16:0 3OH (% 3.27-4.14) ve 16:1 2OH (% 1.08-2.18) yag asitlerinin bu grupta bulunmasidir. Sonuc olarak; Dogu Anadolu Bolgesi’nde Erwinia amylovora’nin streptomisin sulfata karsi dayanikli strainlerinin gelistigi ilk olarak bu calismada tespit edilmistir. Ilave olarak, bu bakterinin FAME gruplari ile streptomisin sulfata karsi dayanikliligi arasinda bir iliskinin olmasindan dolayi; bakterilerin FAME profillerinin laboratuar sartlarinda antibiyotik duyarliliklarinin belirlenmesinde yeni bir yontem olarak kullanilabilecegi dusunulmektedir. Anahtar Kelimeler: Erwinia amylovora, FAME, MIS, streptomisin sulfat, yag asidi Kars and Igdir province, were tested for susceptibilities to streptomycin sulphate. In addition; it was determined to fatty acid methyl esters (FAME) group of the strains, and investigated of relationship between streptomycin susceptibility of them and their FAME group. According to these results, resistance to streptomycin of tested strains was 31.7%. It was determined four different FAMEs groups of strains by using microbial identification system (MIS) system. The difference between groups was changed from 8% to 24%. The highest rate of resistance was in second group (83.3%) and first group (27.6%), and there was not any resistance strains in other groups.. Different of second group from others was that 18:1 w7c fatty acid was the highest level (30.96-43.8%), and there was 16:0 3OH (30.96-43.8%) and 16:1 2OH (1.08-2.18%) fatty acids in only this group. Consequently, this is the first report demonstrating resistance of strains Erwinia amylovora to streptomycin sulphate in Eastern Anatolia Region. In addition, because of the fact that there is a relationship between resistance of bacterial strains to streptomycin sulphate and their FAME profiles, we think that FAME profiles of this bacterium can be used as a new method for determination of bacterial antibiotic resistance in laboratory conditions. Keywords: Erwinia amylovora, FAME, fatty acid, MIS, streptomycin sulphate