Kenichi Nakazato

Association equilibrium of d-methamphetamine and l-methamphetamine with serum albumin

The association equilibria for complex formation between serum albumin (bovine, rat) and the optical isomers of methamphetamine (MAMP) was determined using an ultrafiltration method. It was found that serum albumin/d-MAMP and serum albumin/l-MAMP complexes had distinctly different Scatchard plots with bovine and rat albumin. The binding parameters of each association equilibrium were estimated from the Scatchard plots by Rosenthals graphic method. This distinguished two kinds of specific binding sites in terms of the association equilibrium between bovine serum albumin and d-MAMP, and one binding site for rat serum albumin and d-MAMP. One specific binding site was found between serum albumin and l-MAMP in both bovine and rat. Molar ellipticities, [theta], of peaks were decreased in the CD spectra of the complexes formed between bovine serum albumin and d-MAMP or l-MAMP when compared with the CD spectrum of bovine serum albumin alone. However, no difference in [theta] was found between the CD spectra of the enantiomers of MAMP in the measured wavelength range. The non-specific binding site was distinct from the specific binding site and resulting from altered tertiary structure of the albumin molecule.

Effect of composition of polyacrylamide gels on exclusion limits

Abstract An aqueous ethanol solution of acrylamide, N,N′-methylenebisacrylamide as cross-linked agent, and a third monomer was dispersed in an n -alkane and the three monomers were copolymerized to spherical porous gels. The effect of the composition of the third monomer in the feed on the exclusion limits of the gels was investigated. As the third monomers, acrylonitrile, methacrylamide, N,N-dimethylacrylamide, N,N-dimethylaminopropylacrylamide hydrochloride and N,N-dimethylaminopropylmethacrylamide hydrochloride were used. The logarithm of the exclusion limits of the polyacrylamide terpolymer gels bears a linear relationship to the difference between the values of the solubility parameter of the solvent and gels.

Study of acrylamide gel chromatography. VII. Preparation of polyacrylamide gels by copolymerization with N-hydroxymethylacrylamide and their properties.

N -ヒドロキシメチルアクリルアミドとN, N′-メチレンビスアクリルアミドとを, 希釈剤に水を用いてパラフィン系溶剤中で懸濁重合し, 親水性で多孔性の球状ゲルを得た. 橋かけ剤組成の増加に伴い, 排除限界分子量は増加し8mol%付近以上はほぼ一定の16,000となった. ところが, 希釈剤にエタノールと水の混合液の使用と橋かけ剤組成の増加とにより, 32.0mol%でMR型構造のゲルを得, その排除限界分子量は316,000であり, 膨潤状態での平均細孔径は53.7±4.1Åであった. 希釈剤に水を用いたN-ヒドロキシメチルアクリルアミドとアクリルアミドと75mol%の橋かけ剤からなるポリアクリルアミドゲルで, 膨潤度 (wet-ml/dry-ml), 含水量 (g/dry-g), 排除限界分子量, 比表面積 (mm2/g), 平均細孔径, そしてζ電位はそれぞれ, N-ヒドロキシメチルアクリルアミドとアクリルアミドの和に対するアクリルアミドのモル分率の増加により増大した.