Kenji Fujinuma

肝蛭駆除剤(トリブロムサラン、オキシクロザニドおよびブロムフェノホス)の乳牛への投与後の血漿中濃度および乳汁への移行

The fasciolicides tribromsalan (TBS), oxyclozanide (OCZ) and bromofenofos (BFF) were orally administered to three lactating cows. The concentrations of TBS, OCZ and the BFF metabolite dephosphate bromofenofos (DBFF) in plasma, and the excretion of these compounds in milk were determined by high-performance liquid chromatography. In plasma, the concentrations of TBS, OCZ and DBFF reached maximum at about 1.0 day and the compounds remained detectable until 5.7, 7.4 and 15.1 days after administration, respectively. The detection limits of these compounds in plasma were 10, 2 and 2 ppb, respectively. In milk, the concentrations of TBS, OCZ and DBFF reached maximum at about 24 hours and the compounds remained detectable until 30-47, 30-47 and 78-119 hours after administration, respectively. The detection limits of these compounds in milk were 5.1 and 1 ppb, respectively. The residence times of TBS and BFF were very close to the withdrawal times of the fasciolicides.

Amounts of Acesulfame K, Saccharin and Aspartame Used in Foods

The contents of three sweeteners [acesulfame K (AK), saccharin (SA) and aspartame (APM)] in 203 samples of foods, either imported, purchased on a trip abroad, or made in Japan, were examined. In soft drinks, we detected both SA and APM in three samples manufactured in the U. S. A., AK and APM in three samples manufactured in the U. K. and only APM in three samples manufactured in Japan. In powdered soft drinks, we detected AK and APM, and SA and APM in British and Japanese products, respectively. In solid foods, we detected SA in three pickles and a confectionery (all Japanese-made).Confirmation of AK was carried out as follows: samples were dialyzed against 0.1N hydrochloric acid, and AK was extracted with ethyl acetate from the dialyzate. It was methylated with ethereal diazomethane, and analyzed by gas chromatography-mass spectrometry.