Kenneth J. Piller
Johns Hopkins University School of Medicine
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Featured researches published by Kenneth J. Piller.
The EMBO Journal | 1997
Laura N. Rusché; Jorge Cruz-Reyes; Kenneth J. Piller; Barbara Sollner-Webb
Kinetoplastid mitochondrial RNA editing, the insertion and deletion of U residues, is catalyzed by sequential cleavage, U addition or removal, and ligation reactions and is directed by complementary guide RNAs. We have purified a ∼20S enzymatic complex from Trypanosoma bruceimitochondria that catalyzes a complete editing reaction in vitro. This complex possesses all four activities predicted to catalyze RNA editing: gRNA‐directed endonuclease, terminal uridylyl transferase, 3′ U‐specific exonuclease, and RNA ligase. However, it does not contain other putative editing complex components: gRNA‐independent endonuclease, RNA helicase, endogenous gRNAs or pre‐mRNAs, or a 25 kDa gRNA‐binding protein. The complex is composed of eight major polypeptides, three of which represent RNA ligase. These findings identify polypeptides representing catalytic editing factors, reveal the nature of this ∼20S editing complex, and suggest a new model of editosome assembly.
Molecular and Cellular Biology | 2001
Laura N. Rusché; Catherine E. Huang; Kenneth J. Piller; Michael T. Hemann; Elizabeth Wirtz; Barbara Sollner-Webb
ABSTRACT Kinetoplastid RNA editing is a posttranscriptional insertion and deletion of U residues in mitochondrial transcripts that involves RNA ligase. A complex of seven different polypeptides purified fromTrypanosoma brucei mitochondria that catalyzes accurate RNA editing contains RNA ligases of ∼57 kDa (band IV) and ∼50 kDa (band V). From a partial amino acid sequence, cDNA and genomic clones of band IV were isolated, making it the first cloned component of the minimal RNA editing complex. It is indeed an RNA ligase, for when expressed inEscherichia coli, the protein autoadenylylates and catalyzes RNA joining. Overexpression studies revealed that T. brucei can regulate of total band IV protein at the level of translation or protein stability, even upon massively increased mRNA levels. The proteins mitochondrial targeting was confirmed by its location, size when expressed in T. brucei and E. coli, and N-terminal sequence. Importantly, genetic knockout studies demonstrated that the gene for band IV is essential in procyclic trypanosomes. The band IV and band V RNA ligases of the RNA editing complex therefore serve different functions. We also identified the gene for band V RNA ligase, a protein much more homologous to band IV than to other known ligases.
Molecular Cell | 1998
Jorge Cruz-Reyes; Laura N. Rusché; Kenneth J. Piller; Barbara Sollner-Webb
RNA | 1997
Kenneth J. Piller; Laura N. Rusché; Jorge Cruz-Reyes; Barbara Sollner-Webb
Molecular and Cellular Biology | 1995
Laura N. Rusché; Kenneth J. Piller; Barbara Sollner-Webb
Biochemistry | 1998
Jorge Cruz-Reyes; Kenneth J. Piller; Laura N. Rusché; Mita Mukherjee; Barbara Sollner-Webb
Molecular and Cellular Biology | 1995
Kenneth J. Piller; Carolyn J. Decker; Laura N. Rusché; Michael E. Harris; Stephen L. Hajduk; Barbara Sollner-Webb
Journal of Biological Chemistry | 1996
Kenneth J. Piller; Laura N. Rusché; Barbara Sollner-Webb
Scopus | 1998
Jorge Cruz-Reyes; Laura N. Rusché; Kenneth J. Piller; Barbara Sollner-Webb
Scopus | 1995
Laura N. Rusché; Kenneth J. Piller; Barbara Sollner-Webb