Kenneth W. Tate

Transport of Cryptosporidium parvum Oocysts through Vegetated Buffer Strips and Estimated Filtration Efficiency

ABSTRACT Vegetated buffer strips were evaluated for their ability to remove waterborne Cryptosporidium parvum from surface and shallow subsurface flow during simulated rainfall rates of 15 or 40 mm/h for 4 h. Log10 reductions for spiked C. parvum oocysts ranged from 1.0 to 3.1 per m of vegetated buffer, with buffers set at 5 to 20% slope, 85 to 99% fescue cover, soil textures of either silty clay (19:47:34 sand-silt-clay), loam (45:37:18), or sandy loam (70:25:5), and bulk densities of between 0.6 to 1.7 g/cm3. Vegetated buffers constructed with sandy loam or higher soil bulk densities were less effective at removing waterborne C. parvum (1- to 2-log10 reduction/m) compared to buffers constructed with silty clay or loam or at lower bulk densities (2- to 3-log10 reduction/m). The effect of slope on filtration efficiency was conditional on soil texture and soil bulk density. Based on these results, a vegetated buffer strip comprised of similar soils at a slope of ≤20% and a length of ≥3 m should function to remove ≥99.9% of C. parvum oocysts from agricultural runoff generated during events involving mild to moderate precipitation.

Efficacy of Natural Wetlands to Retain Nutrient, Sediment and Microbial Pollutants

Wetlands can improve water quality through natural processes including sedimentation, nutrient transformations, and microbial and plant uptake. Tailwater from irrigated pastures may contribute to nonpoint source water pollution in the form of sediments, nutrients, and pathogens that degrade downstream water quality. We examined benefits to water quality provided by a natural, flow-through wetland and a degraded, channelized wetland situated within the flood-irrigation agricultural landscape of the Sierra Nevada foothills of Northern California. The non-degraded, reference wetland significantly improved water quality by reducing loads of total suspended sediments, nitrate, and Escherichia coli on average by 77, 60, and 68%, respectively. Retention of total N, total P, and soluble reactive P (SRP) was between 35 and 42% of loads entering the reference wetland. Retention of pollutant loads by the channelized wetland was significantly lower than by the reference wetland for all pollutants except SRP. A net export of sediment and nitrate was observed from the channelized wetland. Decreased irrigation inflow rates significantly improved retention efficiencies for nitrate, E. coli, and sediments in the reference wetland. We suggest that maintenance of these natural wetlands and regulation of inflow rates can be important aspects of a best management plan to improve water quality as water runs off of irrigated pastures.

Spatial and temporal patterns of cattle feces deposition on rangeland

The objective of this study was to identify and model environmental and management factors associated with cattle feces deposition patterns across annual rangeland watersheds in the Sierra Nevada foothills. Daily cattle fecal load accumulation rates were calculated from seasonal fecal loads measured biannually on 40 m2 permanent transects distributed across a 150.5 ha pasture in Madera County, Calif. during the 4 year period from 1995 through 1998. Associations between daily fecal load per season, livestock management, and environmental factors measured for each transect were determined using a linear mixed effects model. Cattle feces distribution patterns were significantly associated with location of livestock attractants, slope percentage, slope aspect, hydrologic position, and season. Transects located in livestock concentration areas experienced a significantly higher daily fecal load compared to transects outside of these concentration areas (P < 0.001). Percent slope was negatively associated with daily fecal load, but this association had a significant interaction with slope aspect (P = 0.02). Daily fecal load was significantly lower during the wet season compared to the dry season (P = 0.002). Daily fecal loading rates across hydrologic positions were dependent upon season. Our results illustrate the opportunities to reduce the risk of water quality contamination by strategic placement of cattle attractants, and provide a means to predict cattle feces deposition based upon inherent watershed characteristics and management factors.

Improved Quantitative Estimates of Low Environmental Loading and Sporadic Periparturient Shedding of Cryptosporidium parvum in Adult Beef Cattle

ABSTRACT Our primary goal was to generate an accurate estimate of the daily environmental loading rate of Cryptosporidium parvum oocysts for adult beef cattle, using immunomagnetic separation coupled with direct immunofluorescence microscopy for a highly sensitive diagnostic assay. An additional goal was to measure the prevalence and intensity of fecal shedding of C. parvum oocysts in pre- and postparturient cows as an indicator of their potential to infect young calves. This diagnostic method could detect with a ≥90% probability oocyst concentrations as low as 3.2 oocysts g of feces−1, with a 54% probability of detecting just one oocyst g of feces−1. Using this diagnostic method, the overall apparent prevalence of adult beef cattle testing positive for C. parvum was 7.1% (17 of 240), with 8.3 and 5.8% of cattle shedding oocysts during the pre- and postcalving periods, respectively. The mean intensity of oocyst shedding for test-positive cattle was 3.38 oocysts g of feces−1. The estimated environmental loading rate of C. parvum ranged from 3,900 to 9,200 oocysts cow−1 day−1, which is substantially less than a previous estimate of 1.7 × 105 oocysts cow−1 day−1 (range of 7.7 × 104 to 2.3 × 105 oocysts cow−1 day−1) (B. Hoar, E. R. Atwill, and T. B. Farver, Quant. Microbiol. 2:21-36, 2000). Use of this highly sensitive assay functioned to detect a greater proportion of low-intensity shedders in our population of cattle, which reduced the estimated mean intensity of shedding and thereby reduced the associated environmental loading rate compared to those of previous studies.

Hydrology in a California oak woodland watershed: a 17-year study

The western foothills of the Sierra-Nevada are some of the most rapidly developing lands in California. Use of these lands includes vineyards, retirement and family home construction, livestock grazing, and fuelwood harvesting. These many uses require varying levels of woodland conversion and oak tree removal that alters the nutrient cycling, wildlife habitat and hydrology of these watersheds. There is little long-term hydrologic data to help determine the impact of these land use changes on water yield or quality. To fill this gap, precipitation and stream flow data were collected for 17 years in a 103 ha California oak woodland watershed, from which oaks were removed from 14% of the land area. These data were combined with measured potential evapotranspiration (PET) to develop a simple water balance and to investigate changes in water yield from oak removal. Hydrologic data included continuous stage height records from a three-foot Parshall flume and a one-foot 908 V-notch weir. Rainfall measurements were made using a tipping bucket rain gage. Average annual rainfall, runoff, and estimated evapotranspiration (ET) for the 17 years were 708, 344, and 364 mm, respectively. In this Mediterranean climate, ET is less dependent upon rainfall than is runoff because the majority of precipitation coincides with the period of lowest PET. Mean annual baseflow depth was 24 mm ranging between 15 and 40 mm. Depth of baseflow was more strongly associated with the annual rainfall than with rainfall from previous years, indicating that changes in soil moisture storage approaches zero on an annual time-scale. Effective depth for watershed soils was calculated to be 217 mm. Potential soil water storage between bedrock and the top of the clay-rich subsoil (Bt Horizon) was 52 mm. This quantity accounts for summer ET and stream baseflow. A weakly significant difference between the pre- and post-harvest mean monthly effective rainfall was observed, indicating that oak removal, from 1984 to 1986, had little influence on watershed hydrology. Peak monthly effective rainfall corresponded to peak monthly runoff. The threshold of response to significantly increase water yield from oak harvesting is greater than 14% of a watershed area for the Sierra-Nevada foothills oak woodlands. q 2000 Elsevier Science B.V. All rights reserved.

Aspen (Populus tremuloides) stands and their contribution to plant diversity in a semiarid coniferous landscape

We conducted a field study to determine the relative contributions of aspen (Populus tremuloides), meadow, and conifer communities to local and landscape-level plant species diversity in the Sierra Nevada and southern Cascade Range, northeastern California, USA. We surveyed plant assemblages at 30 sites that included adjacent aspen, conifer, and meadow communities across a 10,000-km2 region. We statistically investigated patterns in local and landscape-scale plant diversity within and among the three vegetation types. Summing across sites, aspen stands supported more plant species overall and more unique plant species than either meadow or conifer communities. Local richness and diversity did not differ between aspen and meadow plots; conifer forest plots were significantly lower in both measures. Heterogeneity in species composition was higher for aspen forest than for meadows or conifer forest, both within sites and between sites. Plant communities in aspen stands shared less than 25% of their species with adjacent vegetation in conifer and meadow plots. Within aspen forest, we found a negative relationship between total canopy cover and plant diversity. Our results strongly support the idea that plant communities of aspen stands are compositionally distinct from adjacent meadows and conifer forest, and that aspen forests are a major contributor to plant species diversity in the study region. Current patterns of aspen stand succession to conifer forest on many sites in the semiarid western US are likely to reduce local and landscape-level plant species diversity, and may also have negative effects on other ecosystem functions and services provided by aspen forest.

Effect of canopy and grazing on soil bulk density

Abstract This study compared soil surface bulk density between: 1) sites not grazed by cattle > 26 years; 2) sites not grazed for 6 years; 3) sites grazed for 15 years to October residual dry matter levels of > 1100 kg ha−1; 4) sites grazed for 15 years to October residual dry matter levels of 670 to 900 kg ha−1; 5) sites grazed for 15 years to October residual dry matter levels of < 450 kg ha−1; and 6) sites subject to concentrated cattle use (trails, corrals, and supplemental feed-water stations). Sites were collected from across the 1,772 ha San Joaquin Experimental Range (SJER) in Madera County, Calif. to represent canopy cover (open grassland, blue oak (Quercus douglasii Hook and Arn.), live oak (Quercus wislizenii A.DC.), foothill pine (Pinus sabiniana Douglas), wedgeleaf ceanothus (Ceanothus cuneatus (Hook) Nutt.), and ceanothus interspace) and topography (swale, uplands) typical of the rocky coarse sandy loam soils of the southern Sierra Nevada foothill oak savannah. Soil surface (0 to 7.62 cm) bulk density (g cm−3) was determined for 1489 soil cores collected across all available combinations of grazing management, canopy cover and topographic position at the SJER. Soil surface bulk density was 0.23 to 0.30 g cm−3 lower under canopy compared to open grasslands. Bulk density was not different (P > 0.05) between sites not grazed > 26 years and sites not grazed for 6 years. Grazing to residual dry matter levels of > 1100, 670 to 900, and < 450 kg ha−1 created bulk densities which were 0.08, 0.18, and 0.21 g cm−3 greater than non-grazed sites, respectively. Cattle concentration sites had bulk densities 0.37 to 0.47 g cm−3 greater than areas not grazed > 6 or 26 years. For the purpose of maintaining soil surface bulk density current residual dry matter recommendations for sites with canopy cover > 50% appear appropriate, but recommendations for open grasslands need additional review. In particular, residual dry matter level must be directly linked to soil surface infiltration capacity.

Cryptosporidium parvum transport from cattle fecal deposits on California rangelands.

Cryptosporidium parvum is a fecal borne protozoan parasite that can be carried by and cause gastrointestinal illness in humans, cattle, and wildlife. The illness, cryptosporidiosis, can be fatal to persons with compromised immune systems. At question is the potential for C. parvum in cattle fecal deposits on rangeland watersheds to contaminate surface water. First, C. parvum oocysts must be released from fecal deposits during rainfall, becoming available for transport. In 1996, we examined the transport of C. parvum oocysts in overland flow from fecal deposits under natural rainfall and rangeland conditions at the San Joaquin Experimental Range in Madera County, Calif. Our null hypothesis was that C. parvum oocysts are not released from fecal pats and transported 1 m downslope as overland flow with rainfall. Paired plots were located on 10, 20, and 30% slope sites. Each plot was loaded with four, 200 g fecal pats dosed with 10 5 oocysts g -1 . Pats were placed 1.0 m above the base of each plot. Composite runoff samples from each plot were analyzed for oocyst concentration following each of 4 storm events. Oocysts were transported during each storm. Slope was a significant fac tor in oocyst transport, with oocyst transport increasing with slope. Although not significant, there was an apparent flushing effect of oocysts across storms, with the majority transported in the first 2 storms. A pilot rainfall simulation experiment also revealed a flushing phenomenon from pats during individual rainfall events. C. parvum oocysts in fecal pats on rangeland can be transported from fecal deposits during rainfall events, becom ing available for transport to water-bodies. Future studies need to examine surface and subsurface transport of oocysts on range land hillslopes for distances greater than 1 m.

Tallgrass prairie response to grazing system and stocking rate.

Grazing system and stocking rate effects on standing crop of species and relative species composition of tallgrass prairies in north-central Oklahoma were evaluated from 1989 to 1993. Twelve experimental units, consisting of pastures dominated by big bluestem [Andropogon gerurdii Vitman], little bluestem [Schizachyrium scoparium (Michx.) Nash], and indiangrass [Sorghustrum nutuns (L.) Nash], were arranged in a completely randomized design with either a short duration rotation or continuous grazing system and stocking rates ranging from 51.5 animal-unit-days/ha (AUD/ha) to 89.8 AUD/ha. Yearling steers grazed the pastures from late April to late September. Cumulative precipitation was above average during the study period. Continuous and rotation grazing affected the major herbage components similarly over time. Standing crop of all major herbage components declined as stocking rate increased. The standing crop of the major herbage components also declined from the first to the last year of the study. The decrease in standing crop of big bluestem, indiangrass and forbs over years was greatest at lighter stocking rates. Relative composition of switchgrass

Litter contributions to dissolved organic matter and disinfection byproduct precursors in California oak woodland watersheds.

Export of dissolved organic matter (DOM) from California oak woodland ecosystems is of a great concern because DOM is a precursor for carcinogenic disinfection byproducts (DBPs) formed during drinking water treatment. Fresh litter and decomposed duff materials for the four dominant vegetation components of California oak woodlands: blue oak (Quercus douglassi H. & A.), live oak (Quercus wislizenii A. DC.), foothill pine (Pinus sabiniana Dougl.), and annual grasses, were exposed in natural condition for an entire rainy season (December to May) to evaluate their contributions of particulate (POC) and dissolved (DOC) organic carbon, particulate (PON) and dissolved (DON) organic nitrogen, inorganic nitrogen (NH4+ and NO3-), and trihalomethane (THM) and haloacetonitrile (HAN) formation potentials, to surface waters. Litter and duff materials can be significant sources of DOC (litter=29-126 mg DOC g(-1) C; duff=6.5-37 mg DOC g(-1) C) and THMs and HANs (up to 4600 mg-THMs g-C(-1) and 137 microg-HANs g-C(-1)). Blue oak litter had the highest yield of DOC, THM, and HAN precursors. When scaled to the entire watershed, leachate production yielded 445 kg-DOC ha(-1), as compared to DOC export via streams of 5.25 kg-DOC ha(-1). DOC transport to surface waters is facilitated by subsurface lateral flow through A horizons during storm events. The majority of DOM and DBP precursors was leached from plant materials in the initial rainfall events and thus may explain the seasonal stream pattern of a DOC pulse early in the rainy season.