Kessara Pathanapitoon

Uveitis in a tertiary ophthalmology centre in Thailand

Purpose: To determine the aetiology and clinical characteristics of patients with uveitis in a tertiary ophthalmology centre in northern Thailand. Methods: Standard ophthalmological examination and laboratory screening blood tests were performed in 200 consecutive new patients with uveitis. Patients were classified according to the location and aetiology of the uveitis. Specific clinical characteristics were recorded. Design: Prospective case series. Results: The case series included 106 male and 94 female patients with a mean age of 38 years. HIV-associated uveitis was noted in 31% (62/200), and included mostly patients with cytomegalovirus retinitis (85%, 53/62). In the non-HIV group, the most common anatomical type was anterior uveitis (34%, 47/138). Infectious uveitis was diagnosed in 22% (30/138) of non-HIV patients, and toxoplasmosis was the most common infection (12/138, 8.7%). The most common non-infectious clinical entities were Vogt–Koyanagi–Harada disease (20%, 22/108) and HLA-B27-associated acute anterior uveitis (9%, 10/108). Conclusions: The spectrum of uveitis in northern Thailand included 27% of HIV-infected patients with cytomegalovirus retinitis. Causes of non-HIV uveitis were similar to those often observed in the Far East, but the specific prevalences of these disorders were distinct from that found in India and Japan.

Viral causes of unexplained anterior uveitis in Thailand

AimsTo assess the possible role of virus infection in patients with unexplained anterior uveitis (AU).MethodsIntraocular fluid and plasma samples of 30 HIV-negative AU patients who were unresponsive or poorly responsive to topical steroid therapy were analyzed for nucleic acid of cytomegalovirus (CMV), herpes simplex virus (HSV), and varicella zoster virus (VZV) by real-time polymerase chain reaction (PCR) and for intraocular antibodies against these viruses by Goldmann–Witmer coefficient (GWC) analysis. Of these 30 cases, 21 were tested for rubella virus by GWC analysis, 16 of which also had PCR assessment of aqueous for rubella virus.ResultsViral uveitis determined by either real-time PCR and/or GWC was documented in 20 out of 30 patients (67%). Of 30 paired samples tested by both methods for HSV, CMV, and VZV, 15 showed positive results (CMV (10), HSV (4), and VZV (1)). Real-time PCR was positive in 8/15 (53%), whereas GWC was positive in 10/15 (67%). Out of 10 CMV-positive patients, four had endotheliitis, two had Posner–Schlossman syndrome, and one Fuchs heterochromic uveitis syndrome (FHUS). Five out of 21 (24%) samples tested by GWC for Rubella virus were positive, three of which exhibited clinical features of FHUS.ConclusionsOur results indicate that CMV is a major cause of AU in Thailand and show that FHUS can be caused by both CMV and Rubella virus.

Blindness and low vision in a tertiary ophthalmologic center in Thailand: The importance of cytomegalovirus retinitis

Purpose: To determine the causes of blindness and low vision in patients consulting a tertiary ophthalmologic center in northern Thailand. Methods: The study population included 2,951 new consecutive patients from the Department of Ophthalmology at University Hospital in Chiang-Mai, Thailand. Main outcome measures were blindness and low vision, which were defined according to World Health Organization criteria. Results: Of 2,951 patients, 369 (12.5%) had blindness and/or low vision (bilateral blindness in 73, unilateral blindness in 129, bilateral low vision in 77, and unilateral low vision in 90). Of the etiological causes of visual loss, age-related ocular disease was the most frequent (128 patients [35%]) followed by infections (66 patients [18%]) and trauma (43 patients [12%]). Although infections and trauma were the predominant causes of blindness, age-related disorders were frequently found in patients with low vision. Of anatomical sites, the lens (134 patients [36%]) was the main location of visual loss, closely followed by disorders of the retina and/or uvea (126 patients [34%]). Blindness and low vision were considered avoidable in 70% of cases. Of 73 patients with bilateral blindness, 14 had active cytomegalovirus retinitis, accounting for 19% of all patients with bilateral blindness. Conclusion: The most common causes of blindness and low vision in a tertiary center in northern Thailand were age-related ocular disorders and infections, which were predominantly cases of cytomegalovirus retinitis in human immunodeficiency virus–infected patients.

Clinical Manifestations of Cytomegalovirus-Associated Posterior Uveitis and Panuveitis in Patients Without Human Immunodeficiency Virus Infection

IMPORTANCE Little attention has been paid to clinical features of cytomegalovirus (CMV) infections in individuals without human immunodeficiency virus (HIV). OBJECTIVE To describe the clinical manifestations and comorbidities of patients without HIV infection who have CMV-associated posterior uveitis or panuveitis. DESIGN AND SETTING Retrospective observational case series in an academic research setting. PARTICIPANTS The medical records were reviewed of 18 patients (22 affected eyes) diagnosed as having posterior uveitis or panuveitis who had aqueous positive for CMV by polymerase chain reaction techniques. MAIN OUTCOME MEASURES Demographic data, clinical manifestations, and associated systemic diseases were recorded. RESULTS Ocular features included focal hemorrhagic retinitis (n = 13) and peripheral retinal necrosis (n = 7). Two eyes had no focal retinal lesions but manifested vasculitis and vitritis. All patients exhibited vitreous inflammation. Inflammatory reactions in anterior segments developed in 14 of 22 eyes (64%). Retinal vasculitis was observed in 16 of 22 eyes (73%) and included mostly arteries (in 13 of 16 eyes [81%]). Eleven of 18 patients were taking immunosuppressive medications (5 for hematologic malignant diseases, 4 for systemic autoimmune diseases, and 2 following organ transplants). One additional patient was diagnosed as having non-Hodgkin lymphoma 3 months after the onset of CMV-associated panuveitis, and another patient had primary immunodeficiency disorder. Of the remaining 5 patients, 2 had diabetes mellitus, and 3 had no associated systemic diseases and exhibited no evidence of immune deficiency. CONCLUSIONS AND RELEVANCE Cytomegalovirus-associated infections of posterior eye segments can develop in patients without HIV infection who have compromised immune function of variable severity but may occur also in individuals who have no evidence of immune insufficiency. Cytomegalovirus infections located in posterior eye segments in patients without HIV infection caused intraocular inflammatory reaction in all cases and demonstrated more variable clinical presentation than classic CMV retinitis observed in patients with HIV infection.

HLA-B27-associated acute anterior uveitis in the University Referral Centre in North Thailand: clinical presentation and visual prognosis

Background: Acute anterior uveitis (AAU) is the most frequent type of uveitis encountered in the west. Although human leucocyte antigen (HLA)-B27-associated ankylosing spondylitis was reported in South East Asia, it is not known whether HLA-B27-associated ocular disease is prevalent in Thailand. Methods: A prospective study of 100 unrelated blood donors and 121 consecutive patients with AAU was carried out. All people underwent HLA-B27 typing and full ocular examination. Radiological examination of the sacroiliac joints was conducted in patients with low back pain or arthralgias. Results: The prevalence of HLA-B27 was 10% among the blood donors in contrast with 44% in the AAU group (p<0.001). The clinical characteristics of HLA-B27-associated AAU were similar to those published throughout the world (unilaterality in 74%, hypopyon in 31%, recurrent AAU in 64%). However, the increased intraocular pressure (IOP) was more common in the HLA-B-27-negative group (p = 0.03) than in their HLA-B27-positive counterparts. At least 15% of the HLA B27-positive group had radiological signs of ankylosing spondylitis. Conclusion: The prevalence of HLA-B27 in the population without uveitis in Thailand is about 10% and clinical characteristics of HLA-B27-positive AAU are similar to those reported in the west. In contrast with earlier reports, HLA-B27-negative AAU in Thailand was associated with increased IOP and should be further studied.

Intraocular and plasma HIV-1 RNA loads and HIV uveitis.

Objective:The objective of this study was to analyze human immunodeficiency virus (HIV) dynamics across the blood–retinal barrier and to determine whether the high levels of HIV in the eye are associated with any ocular disorders in HIV-infected patients. Design:This study included a prospective case series of 40 HIV-positive patients with uveitis. Intervention:Clinical and laboratory examinations included plasma and intraocular HIV-1 RNA loads as well as the clinical manifestations of uveitis. Results:Intraocular HIV-1 RNA was detected in 32% (13/40) of HIV-positive patients with uveitis. Intraocular HIV-1 RNA loads were associated with high HIV-1 RNA plasma loads (P < 0.001) and not being on HAART therapy (P = 0.005). In addition, detectable intraocular HIV-1 RNA levels were higher in patients with the absence of retinal lesions (P = 0.008). In three patients, the HIV load in the eye largely exceeded that of plasma. These three patients had all bilateral anterior uveitis and/or vitritis without retinal lesions and exhibited no evidence of other intraocular infectious agents causing uveitis than HIV itself. Conclusion:The eye can form a sanctuary where HIV might replicate and cause an inflammatory reaction.

Chronic Central Serous Chorioretinopathy Associated with Serous Retinal Detachment in a Series of Asian Patients

Purpose: To determine clinical features of patients with severe chronic central serous chorioretinopathy (diffuse retinal pigment epitheliopathy, DRPE) associated with bullous retinal detachment in Thailand. Methods: The authors reviewed clinical and imaging characteristics, visual outcomes, and complications of 7 patients with severe DRPE associated with bullous retinal detachment. Results: Included were 6 males and 1 female with average age at onset of 39 years (range 30–46 years) diagnosed with DRPE. Although 4 patients had unilateral complaints, retinal pigment epithelium (RPE) changes on fluorescein angiography (FA) were visible in both eyes in all patients and 10 out of 14 affected eyes exhibited large exudative bullous retinal detachments (RD) and evidence of multiple characteristic leakage points. The disease was induced by steroid medications in 3 patients and an additional 3 patients received steroid treatment after they were initially considered to have Harada disease. The administration of steroids caused worsening in all cases. Conclusion: Chronic central serous chorioretinopathy associated with bullous retinal detachment is a severe variant of DRPE, which might be mistaken for Harada disease. The early diagnosis of DRPE might prevent the complications from harmful medications as well as unnecessary surgery and visual loss.

Clinical Manifestations of Human Immunodeficiency Virus-Induced Uveitis

PURPOSE To describe the clinical manifestations of patients with human immunodeficiency virus (HIV)-induced uveitis in Thailand. DESIGN Prospective cohort study of 6 patients with HIV-induced uveitis. PARTICIPANTS Six patients (8 eyes) with HIV-induced uveitis who had an extremely high intraocular: plasma HIV-1 RNA ratio. METHODS The clinical manifestations and laboratory findings are reported of 6 consecutive patients with HIV-induced uveitis who had an extremely high intraocular-to-plasma HIV-1 RNA ratio and were diagnosed between July 2009 and May 2011. MAIN OUTCOME MEASURES Clinical manifestations and laboratory findings. RESULTS Human immunodeficiency virus-induced uveitis was diagnosed in 4 men and 2 women with an average age of 41 years at presentation. None of the patients were receiving highly active anti-retroviral therapy (HAART) or had clinical or laboratory evidence, or both, of opportunistic infections. The mean plasma load was 218 688 copies/ml (median, 137 500 copies/ml; range, 24 900-540 000 copies/ml), and the mean intraocular HIV load was 20 937 755 copies/ml (median, 7 499 000 copies/ml; range, 2 460 000-89 800 000 copies/ml). The average CD4 cell count was 192 cells/μl (median, 248 cells/μl; range, 5-342 cells/μl). All the patients had decreased vision, and none had conjunctival hyperemia. The anatomic location of uveitis was anterior in all patients, and associated vitreitis was present in 4 patients; none exhibited retinal lesions or scars. Anterior segment inflammation and keratic precipitates were observed in all patients, and none responded to topical corticosteroid therapy. After the administration of HAART, the intraocular inflammation disappeared entirely within several weeks in all of the patients and the intraocular and plasma HIV loads decreased. CONCLUSIONS Human immunodeficiency virus-induced uveitis should be suspected in HAART-naïve, HIV-positive patients or in those in whom this treatment fails and who have anterior uveitis without any retinal lesions and exhibit no response to topical corticosteroids. The concurrent determination of HIV load in the intraocular fluids and plasma may clarify the cause of HIV-associated uveitis.

The diagnostic value of intraocular fluid analysis by polymerase chain reaction in Thai patients with uveitis

Uveitis is a major cause of severe visual impairment throughout the world and can be initiated by various infectious and non-infectious causes. Early recognition of specific infections is important as the treatment with antimicrobial agents might stop the progression or even cure the eye disease. To determine the infectious causes of uveitis in Thailand, intraocular fluid samples of 100 HIV-negative patients and 47 HIV-positive patients with uveitis were examined using real-time PCR analysis for herpes simplex virus, varicella zoster virus, cytomegalovirus and Toxoplasma gondii. Positive PCR results were found in 33/100 (33%) HIV-negative patients and in 33/47 (70%) HIV-positive patients with uveitis. In Thailand, cytomegalovirus was identified as the most frequent cause of infectious uveitis in both HIV-negative and HIV-positive patients (49 and 91%, respectively). PCR analysis of intraocular samples in uveitis was a valuable diagnostic assay. The pattern of uveitis observed in the Far East differs from that found in the West.

Keratometry and axial length in pigment dispersion syndrome: a descriptive case-control study.

PurposePigment dispersion syndrome and pigmentary glaucoma are characterized by loss of pigment from the posterior surface of the iris due to reverse pupillary block. This may be due to an anomalous relation between the position of the globe within the orbit, the axial length of the globe, and the corneal curvature. The authors compared axial length, keratometry, and exophthalmometry in patients with pigment dispersion syndrome and pigmentary glaucoma and in healthy controls. MethodsThirteen pigment dispersion syndrome and pigmentary glaucoma and 17 controls underwent refraction, keratometry, A-scan and exophthalmometry. Patients with pigment dispersion syndrome and pigmentary glaucoma were selected in a university glaucoma practice, and controls were selected for their age and refraction from the staff of the hospital. Both groups were comparable in age and refraction. ResultsMean age of pigment dispersion syndrome patients and healthy controls was 46.54 and 41.82 years (P = 0.30), ,respectively, and mean refraction (spherical equivalent) was −4.53 and −4.32 diopters, (P = 0.84), respectively. No statistical differences were noted in axial length measured with A-scan (25.98 and 25.14, P = 0. 15) or anterior chamber depth and lens thickness. The pigment dispersion syndrome and pigmentary glaucoma group had flatter keratometry than the myopic controls. Mean keratometry was 42.39 ± 1.77 and 44.34 ± 1.50 (P = 0.003), respectively. ConclusionThe results show that patients with pigment dispersion syndrome have flatter keratometry of approximately 2 diopters compared with age-matched myopic controls. This finding suggests a difference in architecture of the anterior segment.