Khansa Taha Ababneh

Oral pyogenic granuloma in jordanians: a retrospective analysis of 108 cases

Abstract Purpose: The purpose of the present study was to retrospectively analyze the clinicopathologic features and treatment of oral pyogenic granuloma in Jordanian patients. Patients and methods: Information regarding 108 cases of pyogenic granuloma of the oral cavity that underwent biopsy were retrieved from the records of patients seen at the maxillofacial and periodontology units of the Faculty of Dentistry, Jordan University of Science and Technology, during an 11-year period from 1991 to 2001. Data were reviewed and analyzed for age, gender, site, treatment, and clinical and histopathologic features. Results: Patient ages ranged from 3 to 85 years (mean, 30 years), with the greatest degree of occurrence (26.8%) in the second decade. The male-to-female ratio was 1:1.7. The mean age for females age was higher than that for males. The most frequently involved site was the gingiva (44.4%); other sites were the lips, tongue, buccal mucosa, and palate. Gingival pyogenic granulomas were more prevalent in the maxilla than in the mandible, with the anterior region of both jaws being more commonly affected. The labiobuccal gingiva of both jaws was more commonly affected. The main complaint was bleeding (59.3%), and almost half of the lesions had a pedunculated base, with surface ulceration in 9.2% of cases. The mean greatest granuloma diameter was 10 mm. All lesions were surgically excised, with 5.8% of cases known to have recurred. Conclusions: The clinicopathologic features of oral pyogenic granuloma in Jordanians are similar to those of other whites. In this series, we found that with surgical excision of pyogenic granuloma, there was a low recurrence rate.

Position of the mental foramen in a northern regional Jordanian population

BackgroundThe mental foramen is frequently encountered in a number of maxillofacial surgical procedures. Its position has been shown to vary according to race. The aim was to study the position, shape, and appearance of the mental foramen, as seen on panoramic radiographs of Jordanians, and to compare our findings with international values.Patients and methodsPanoramic radiographs were randomly selected from the records of dental patients attending three dental services, and were analyzed according to patients’ age and gender, and the mental foramina’s anterior–posterior and superior–inferior positions, shape, appearance, and symmetry.ResultsThere were 860 cases (1,720 sides) with a female-to-male ratio of 1:1.4, and mean age of 24. The most frequent anterior–posterior position was in the area between the long axes of first and second mandibular premolar teeth. With advancing age, there was an increase in the frequency of more posterior positioning. The anterior–posterior position was asymmetrical in 33% of cases. The most frequent superior–inferior position was below the level of apices of mandibular premolar teeth roots. With advancing age there was an increase in the frequency of more inferior positioning. The superior–inferior position was asymmetrical in 14% of cases. The majority of foramina were round in shape, and the most frequent appearance was the continuous type. Accessory mental foramina were seen in 10% of the cases.ConclusionThe position of the mental foramen on panoramic radiographs in this selected group of Jordanians is most commonly below and between the mandibular premolar teeth, and the most frequent appearance was the continuous type. These results are similar to previous findings in Caucasian populations.

Prevalence and risk indicators of gingivitis and periodontitis in a Multi-Centre study in North Jordan: a cross sectional study

BackgroundThere are limited data about the epidemiology and risk factors/indicators of gingivitis, aggressive periodontitis (AgP) and chronic periodontitis (CP) in Jordan. The aim of this study was to assess the prevalence and risk indicators of gingivitis, AgP and CP.MethodsA sample of 595 subjects was randomly selected from subjects escorting out-patients attending a Medical Center, a Dental Teaching Hospital, and 2 private dental clinics. The socio-demographic variables, oral hygiene habits, income, smoking and Body Mass Index (BMI) were recorded. Full mouth periodontal examination was performed, and radiographs were taken for sites with probing depth > 3 mm.ResultsAbout 76% had gingivitis, 2.2% had AgP and 5.5% had CP. Periodontitis was more frequent among males than females with a M: F ratio of 1.6:1 and the prevalence increased with age. Subjects who reported not using a tooth brush, smokers and subjects with BMI > 30 kg/m2 had significantly higher prevalence of periodontitis. The risk for periodontitis was greater among subjects who reported positive family history and subjects with ≤ 12 years of education.ConclusionsThis is the first study to report on the prevalence of gingivitis, CP and AgP in North Jordanian. Age, low education, low frequency of tooth brushing and family history were significantly associated with increased risk of periodontitis.

Association of interleukin-10 gene promoter polymorphisms with chronic and aggressive periodontitis.

OBJECTIVE Interleukin-10 gene promoter polymorphisms have been associated with interleukin-10 decreased production, thereby playing a role in the pathogenesis of periodontitis. This study aimed to investigate whether interleukin-10 single nucleotide polymorphisms at positions -1087(G/A) and -597(C/A) are associated with generalised chronic periodontitis and localised aggressive periodontitis. METHODS Genomic DNA samples were isolated from 276 unrelated Jordanian participants. Subjects were categorised into 86 periodontally healthy controls, 105 chronic periodontitis patients and 85 localised aggressive periodontitis patients. Genotype frequencies were calculated, and differences were determined using Pearson chi-squared test, and odds ratio and 95% confidence intervals were included. RESULTS The frequencies of the -1087A and -597A alleles were significantly more common in chronic periodontitis patients than controls. The A-positive allele genotypes (GA, AA) at position -1087 and A-positive allele genotypes (CA, AA) at position -597 appeared to increase the risk of having chronic periodontitis. No significant differences were observed in the genotype frequencies between localised aggressive periodontitis patients and controls. CONCLUSIONS These findings indicate the possible use of interleukin-10 single nucleotide polymorphisms as genetic markers in chronic periodontitis patients and further emphasise the molecular differences between chronic periodontitis and aggressive periodontitis.

Investigation of the interleukin-1 gene cluster polymorphisms in Jordanian patients with chronic and aggressive periodontitis.

OBJECTIVE Interleukin-1 (IL-1) is a proinflammatory cytokine that is highly elevated in response to bacterial biofilms and is a potential risk factor for periodontal diseases. IL-1 gene polymorphisms have been associated with the IL-1 level. The aim of this study was to investigate if IL-1 gene cluster polymorphisms are associated with chronic (CP) and aggressive (AgP) periodontitis in a Jordanian population. METHODS A total of 100 CP, 80 AgP patients and 80 controls were genotyped using PCR for IL-1RN-86-bp VNTR and PCR-RFLP for IL-1A-889, IL-1B-511, -35, +3953, and IL-1RN +8006, +9589, +11100 SNPs. The distribution of alleles and genotypes between groups was compared using χ(2) analysis. Estimation of haplotype frequencies was carried out using the EH programme. RESULTS The IL-1RN8006 SNP and the IL-1RN-VNTR were associated with CP but not with AgP. The C allele and TC genotype of IL-1RN8006 were increased in CP (P(corr)=0.002, 0.00026 respectively). The A1 allele and A1/A1 genotype of the IL1-RN-VNTR were significantly increased in CP (P(corr)=0.0007, <0.0001 respectively). The CA1 haplotype formed by both markers was present in 29 CP patients but not in any of the controls (P<0.0001). No significant differences were found in the distribution of allele and genotype frequencies of the other markers between CP and AgP cases and controls. CONCLUSIONS IL-1RN 8006 and IL-1RN VNTR were associated with CP but not AgP in a Jordanian population, whilst other investigated markers in IL-1A, IL-1B and IL-1RN were not associated with either CP or AgP.

Association of vitamin D receptor gene polymorphisms with chronic and aggressive periodontitis in Jordanian patients

Vitamin D acts through binding with vitamin D receptor (VDR) and is responsible for regulating bone metabolism and mineralization; it also suppresses the immune system. The aim of this study was to investigate if VDR gene polymorphisms are associated with chronic periodontitis (CP) and aggressive periodontitis (AgP) in a Jordanian population. A total of 99 patients with CP, 63 patients with AgP, and 126 controls were genotyped using PCR-restriction fragment length polymorphism (RFLP) for BsmI, ApaI, and TaqI single nucleotide polymorphisms (SNPs). The association was determined after correcting for confounding factors using multivariate logistic regression analysis. Estimation of haplotype frequencies was carried out using the EH program, and haplotypes were constructed using the phase 2.1 program. After correcting for confounding factors, multivariate logistic regression analysis revealed that inheritance of the BsmI bb genotype or the ApaI aa genotype was associated with increased risk of developing CP (OR = 2.4 and OR = 3.4, respectively) but with reduced risk of developing AgP (OR = 0.4 and OR = 0.3, respectively). This was further supported by association of the ba haplotype with CP but not with AgP. This study supports an association of VDR gene polymorphisms with CP and AgP in a Jordanian population; however, the pattern of association was different between the two diseases.

The association of aggressive and chronic periodontitis with systemic manifestations and dental anomalies in a jordanian population: a case control study

BackgroundThe relationship between dental anomalies and periodontitis has not been documented by earlier studies. Although psychological factors have been implicated in the etiopathogenesis of periodontitis, very little information has so far been published about the association of anxiety and depression with aggressive periodontitis. The aim of this study was to investigate the association of chronic periodontitis and aggressive periodontitis with certain systemic manifestations and dental anomalies.MethodsA total of 262 patients (100 chronic periodontitis, 81 aggressive periodontitis and 81 controls), attending the Periodontology clinics at Jordan University of Science and Technology, Dental Teaching Centre) were included. All subjects had a full periodontal and radiographic examination to assess the periodontal condition and to check for the presence of any of the following dental anomalies: dens invaginatus, dens evaginatus, congenitally missing lateral incisors or peg-shaped lateral incisors. Participants were interrogated regarding the following: depressive mood, fatigue, weight loss, or loss of appetite; and their anxiety and depression status was assessed using the Hospital Anxiety and Depression (HAD) scale.ResultsPatients with aggressive periodontitis reported more systemic symptoms (51%) than the chronic periodontitis (36%) and control (30%) patients (p < 0.05). Aggressive periodontitis patients had a higher tendency for both anxiety and depression than chronic periodontitis and control patients. Dental anomalies were significantly (p < 0.05) more frequent among both of chronic and aggressive periodontitis patients (15% and 16%, respectively), compared to controls.ConclusionIn this group of Jordanians, systemic symptoms were strongly associated with aggressive periodontitis, and dental anomalies were positively associated with both aggressive and chronic periodontitis.

Immunolocalization of Proteoglycans in Meckel’s Cartilage of the Rat

The aim of this study was to investigate the presence and distribution of proteoglycans within Meckel’s cartilage of rat embryos. A standard indirect immunoperoxidase technique was used on paraffin sections of rat heads. Sections were incubated with monoclonal antibodies recognising core protein epitopes in the proteoglycans versican and CD44. Polyclonal antibodies localized the proteoglycans decorin, biglycan and lumican. Versican was expressed by chondrocytes, but very weekly by the extracellular matrix. Decorin was strongly expressed by both of chondrocytes and the ECM. Both of biglycan and lumican were moderately expressed by chondrocytes, but weakly by the extracellular matrix. CD44 was weakly expressed by chondrocytes only, without staining of the ECM. It is concluded that Meckel’s cartilage chondrocytes express the proteoglycans versican, decorin, biglycan, lumican and CD44 at variable levels during development in the rat. Such data are important for a greater understanding of the changes that take place during mandibular development. Further studies are needed to elucidate the exact role of proteoglycans during Meckel’s cartilage and mandibular organogenesis.