Ki Mo Jang

Three-dimensional bioprinting of multilayered constructs containing human mesenchymal stromal cells for osteochondral tissue regeneration in the rabbit knee joint

The use of cell-rich hydrogels for three-dimensional (3D) cell culture has shown great potential for a variety of biomedical applications. However, the fabrication of appropriate constructs has been challenging. In this study, we describe a 3D printing process for the preparation of a multilayered 3D construct containing human mesenchymal stromal cells with a hydrogel comprised of atelocollagen and supramolecular hyaluronic acid (HA). This construct showed outstanding regenerative ability for the reconstruction of an osteochondral tissue in the knee joints of rabbits. We found that the use of a mechanically stable, host-guest chemistry-based hydrogel was essential and allowed two different types of extracellular matrix (ECM) hydrogels to be easily printed and stacked into one multilayered construct without requiring the use of potentially harmful chemical reagents or physical stimuli for post-crosslinking. To the best of our knowledge, this is the first study to validate the potential of a 3D printed multilayered construct consisting of two different ECM materials (atelocollagen and HA) for heterogeneous tissue regeneration using an in vivo animal model. We believe that this 3D printing-based platform technology can be effectively exploited for regeneration of various heterogeneous tissues as well as osteochondral tissue.

Posterior Multilevel Vertebral Osteotomy for Correction of Severe and Rigid Neuromuscular Scoliosis: A Preliminary Study

Study Design. Prospective study. Objective. To determine the effectiveness and correction with posterior multilevel vertebral osteotomy in severe and rigid curves without anterior release. Summary of Background Data. For the correction of severe and rigid scoliotic curve, anterior-posterior combined or posterior vertebral column resection (PVCR) procedures are used. Anterior procedure might compromise pulmonary functions, and PVCR might carry risk of neurologic injuries. Therefore, authors developed a new technique, which reduces both. Methods. Thirteen neuromuscular patients (7 cerebral palsy, 2 Duchenne muscular dystrophy, and 4 spinal muscular atrophy) who had rigid curve >100° were prospectively selected. All were operated with posterior-only approach using pedicle screw construct. To achieve desired correction, posterior multilevel vertebral osteotomies were performed at 3 to 5 levels (apex, and 1–2 levels above and below apex) through partial laminotomy sites connecting from concave to convex side, just above the pedicle; and repeated cantilever manipulation was applied over temporary short-segment fixation, above and below the apex, on convex side. On concave side, rod was assembled with screws and rod-derotation maneuver was performed. Finally, short-segment fixation on convex side was replaced with full-length construct. Intraoperative MEP monitoring was applied in all. Results. Mean age was 21 years and average follow-up was 25 months. Average preoperative flexibility was 20.3% (24.1°). Average Cobb’s angle, pelvic obliquity, and apical rotation were 118.2°, 16.7°, and 57° preoperatively, respectively, and 48.8°, 8°, and 43° after surgery showing significant correction of 59.4%, 46.1%, and 24.5%. Average number of osteotomy level was 4.2 and average blood loss was 3356 ± 884 mL. Mean operation time was 330 ± 46 minutes. None of the patient required postoperative ventilator support or displayed any signs of neurologic or vascular injuries during or after the operation. Conclusion. This technique should be recommended because (1) it provides release of anterior column without anterior approach and (2) our results supports its superiority as a technique.

Quantitative assessment of mineralization in distraction osteogenesis

ObjectivesThe most important decision in distraction osteogenesis is the timing of fixator removal. Various methods have been tried, such as radiographic appearance of callus and bone mineral density (BMD) assessment, but none has acquired gold standard status. The purpose of this study was to develop another objective method of assessment of callus stiffness to help clinicians in taking the most important decision of when to remove the fixator.Materials and methodsWe made a retrospective study of 70 patients to compare the BMD ratio and pixel value ratio. These ratios were calculated at the time of fixator removal, and Pearson’s coefficient of correlation was used to show the comparability. Inter- and intra-observer variability of the new method was also tested.ResultsGood correlation was found between BMD ratio and pixel value ratio, with a Pearson’s coefficient of correlation of 0.79. The interobserver variability was also low, with high intra-observer reproducibility, suggesting that this test was simple to perform.ConclusionPixel value ratio is a good method for assessing callus stiffness, and it can be used to judge the timing of fixator removal.

Are Navigation Systems Accurate Enough to Predict the Correction Angle During High Tibial Osteotomy? Comparison of Navigation Systems With 3-Dimensional Computed Tomography and Standing Radiographs

Background: Unpredicted overcorrection of the mechanical axis can occur during navigation-assisted high tibial osteotomy (HTO). It is not clear whether the erroneous overcorrection stems from the navigation system itself or from other causes. Purpose: To evaluate the accuracy of the navigation system in HTO by comparing the change in the femorotibial angle provided by the navigation system with the bony correction angle of the proximal tibia on 3-dimensional computed tomography (3D CT) and with the change in mechanical femorotibial alignment on standing whole-leg radiographs. Study Design: Cohort study (diagnosis); Level of evidence, 2. Methods: A total of 16 knees underwent navigation-assisted HTO, and their alignment data were obtained before and after correction. For comparison, preoperative and follow-up standing whole-leg anteroposterior radiographs, lateral knee radiographs, and preoperative and postoperative 3D CT scans were taken. The medial proximal tibial angle (MPTA), posterior tibial slope, and mechanical femorotibial angle (mFTA) were measured in these images, and the 3 coronal and 3 sagittal correction angles were compared with each other. Results: In the coronal plane, the mean correction angle of the navigation system was 9.3° ± 2.0° valgus (range, 6°-13°), and the mean MPTA on 3D CT increased 9.7° ± 2.0° (range, 6.7°-13.8°) after correction. The mean correction angle of the mFTA on standing radiographs was 11.9° ± 3.2° valgus (range, 6.9°-16.5°). There was no statistical significance between the navigation system and 3D CT (P = .187), but there was a statistically significant difference between the navigation system and standing radiographs (P = .001). The results of the correction angle in the sagittal plane were similar to those in the coronal plane. Conclusion: The correction of the femorotibial angle by the navigation system was not different from the bony correction angle on 3D CT. There was a discrepancy between the correction angle of the navigation system and that of the standing radiograph. Clinical Significance: Surgeons must be cautious about the tension of soft tissue, even when using the navigation system during HTO.

Differentiating xanthogranulomatous cholecystitis from wall-thickening type of gallbladder cancer: Added value of diffusion-weighted MRI

AIM To evaluate the benefit of diffusion-weighted imaging (DWI) in differentiating xanthogranulomatous cholecystitis from the wall-thickening type of gallbladder cancer. MATERIALS AND METHODS This retrospective study was approved by the institutional review board. Fourteen patients with xanthogranulomatous cholecystitis and 19 patients with the wall-thickening type of gallbladder cancer were included. Qualitative (visual diffusion restriction compared to liver parenchyma) and quantitative [apparent diffusion coefficient (ADC)] analyses were performed. Conventional MRI findings including dynamic enhancement pattern between the two groups were also analysed. Two observers independently reviewed conventional magnetic resonance imaging (MRI) images and subsequently reviewed combined conventional MRI and DWI images. Pairwise comparison of the receiver operating characteristic (ROC) curves was used to compare diagnostic performances. RESULTS In conventional MRI findings, xanthogranulomatous cholecystitis showed significant continuity of enhancing mucosal line [79% (11/14) versus 26% (5/19), p = 0.003] and intramural T2-high signal intensity [64% (9/14) versus 21% (4/19), p = 0.012] compared to the wall-thickening type of gallbladder cancer. The enhancement pattern of gallbladder cancer compared to liver parenchyma showed earlier onset than that of xanthogranulomatous cholecystitis (p = 0.001). Diffusion restriction was more frequently seen in the wall-thickening type of gallbladder cancer (68%, 13/19) than in xanthogranulomatous cholecystitis (7%, 1/14; p < 0.001). The mean ADC value of xanthogranulomatous cholecystitis was higher than that of the wall-thickening type of gallbladder cancer with statistical significance (1.637 × 10(-3) mm(2)/s versus 1.076 × 10(-3) mm(2)/s, p = 0.005). Diagnostic performance [area under ROC curve (Az)] of both observers improved significantly after additional review of DWI; Az improved from 0.737 to 0.930 (p = 0.027) for observer 1 and from 0.675 to 0.938 (p = 0.008) for observer 2. CONCLUSION Addition of DWI to conventional MRI improves discrimination between xanthogranulomatous cholecystitis and the wall-thickening type of gallbladder cancer.

Pathological correlation with diffusion restriction on diffusion-weighted imaging in patients with pathological complete response after neoadjuvant chemoradiation therapy for locally advanced rectal cancer: preliminary results

OBJECTIVE The objective of this study was to assess causative pathological factors associated with diffusion restriction on diffusion-weighted imaging (DWI) in patients who achieved pathological complete response (pCR) after treatment with neoadjuvant chemoradiation therapy (CRT) for locally advanced rectal cancer. METHODS In total, 43 patients with locally advanced rectal cancer (≥T3 or lymph node positive) who underwent neoadjuvant CRT, subsequent surgery and ultimately achieved pCR were enrolled. All patients underwent pre- and post-CRT 3.0 T rectal MRI with DWI. Two radiologists blinded to pathological staging reviewed pre- and post-CRT 3.0 T rectal MRI for the presence of diffusion restriction in the corresponding tumour areas on post-CRT DWI, with a third radiologist arbitrating any disagreement. The consensus of these findings was then correlated with pathological data such as intramural mucin and the degree of proctitis and mural fibrosis seen on surgical specimen. Additionally, the pre-CRT tumour volume was measured to define the effect of this variable on the degree of radiation proctitis and fibrosis, as well as the presence of intramural mucin. RESULTS Diffusion restriction occurred in 18 subjects (41.9%), while 25 subjects remained diffusion restriction-free (58.1%). The diffusion restriction group tended to have more severe proctitis and mural fibrosis when compared with non-diffusion restriction group (p<0.001). Intramural mucin was also more common in the diffusion restriction group (p=0.052). Higher pre-CRT tumour volumes were significantly predictive of the degree of proctitis (p=0.0247) and fibrosis (p=0.0445), but not the presence of intramural mucin (p=0.0944). Proctitis and mural fibrosis severity were also identified as independent pathological risk factors for diffusion restriction on multivariate analysis (p=0.0073 and 0.0011, respectively). CONCLUSION Both radiation-induced proctitis and fibrosis were significant and independent predictors of diffusion restriction in patients achieving pCR after treatment with neoadjuvant CRT for locally advanced rectal cancer, and pre-CRT tumour volume significantly affects both variables.

The Use of Contralateral Knee Magnetic Resonance Imaging to Predict Meniscal Size During Meniscal Allograft Transplantation

PURPOSE The purpose of this study was to determine the size of each meniscus and compare it with the contralateral limb using conventional knee magnetic resonance imaging (MRI) taken from previously uninjured, healthy, young volunteers. METHODS The knee joints of 60 healthy volunteers (aged 21 to 43 years, 30 men and 30 women) were enrolled in this study. Standard 3.0-T MRI in a controlled setting was used. By use of the mid-coronal images, the height and width of each medial and lateral meniscus were measured. By use of the mid-sagittal images, the height and width of the anterior and posterior horns of each meniscus was measured. The whole length spanning from the most anterior margin to the most posterior margin of each meniscus was also measured. Mean, standard deviation, and 95% confidence interval values were determined for each measurement. RESULTS There were 3 incomplete discoid lateral menisci (10%) in men and 2 incomplete discoid menisci (6.7%) in women. The study group with non-discoid knees comprised 27 men and 28 women. The power of this study ranged from 0.57 to 0.66. All values showed good reliability (intraclass correlation coefficient range, 0.887 to 0.974). There were no significant differences between right and left menisci (all P > .05). There were significant differences between genders. All parameters showed significant differences (P < .05) except the medial meniscus width (P = .221). CONCLUSIONS In this small subset of patients, there were no differences between right and left meniscal measurements according to MRI. Therefore, when one is performing meniscal allograft transplantation, contralateral knee MRI may be useful to determine the required size. Identifying both the overall width and length of each meniscus is important when preparing an allograft. CLINICAL RELEVANCE Contralateral knee MRI may be used for more accurate meniscal size measurement in patients undergoing meniscal allograft transplantation.

Unintended Rotational Changes of the Distal Tibia After Biplane Medial Open-Wedge High Tibial Osteotomy.

This study involved 35 knees undergoing biplane medial open-wedge high tibial osteotomy (OWHTO) to assess the axial rotation of the distal tibia. The distal tibiae were internally rotated by 3.0° ± 7.1° after OWHTO. The opening width showed a Pearson correlation coefficient of -0.743 (P < .001), and the tuberosity osteotomy angle showed that of -0.678 (P < .001) with distal tibial rotation. However, changes in hip-knee-ankle angle, medial proximal tibial angle, and posterior tibial slope were not significantly correlated with the change in distal tibial rotation. In conclusion, there was an unintended tendency of increasing internal rotation of the distal tibia after biplane medial OWHTO, and this tendency was positively related to the opening width and tuberosity osteotomy angle.

In Vivo Three-Dimensional Imaging Analysis of Femoral and Tibial Tunnel Locations in Single and Double Bundle Anterior Cruciate Ligament Reconstructions

Background Anatomic footprint restoration of anterior cruciate ligament (ACL) is recommended during reconstruction surgery. The purpose of this study was to compare and analyze the femoral and tibial tunnel positions of transtibial single bundle (SB) and transportal double bundle (DB) ACL reconstruction using three-dimensional computed tomography (3D-CT). Methods In this study, 26 patients who underwent transtibial SB ACL reconstruction and 27 patients with transportal DB ACL reconstruction using hamstring autograft. 3D-CTs were taken within 1 week after the operation. The obtained digital images were then imported into the commercial package Geomagic Studio v10.0. The femoral tunnel positions were evaluated using the quadrant method. The mean, standard deviation, standard error, minimum, maximum, and 95% confidence interval values were determined for each measurement. Results The femoral tunnel for the SB technique was located 35.07% ± 5.33% in depth and 16.62% ± 4.99% in height. The anteromedial (AM) and posterolateral (PL) tunnel of DB technique was located 30.48% ± 5.02% in depth, 17.12% ± 5.84% in height and 34.76% ± 5.87% in depth, 45.55% ± 6.88% in height, respectively. The tibial tunnel with the SB technique was located 45.43% ± 4.81% from the anterior margin and 47.62% ± 2.51% from the medial tibial articular margin. The AM and PL tunnel of the DB technique was located 33.76% ± 7.83% from the anterior margin, 45.56% ± 2.71% from the medial tibial articular margin and 53.19% ± 3.74% from the anterior margin, 46.00% ± 2.48% from the medial tibial articular margin, respectively. The tibial tunnel position with the transtibial SB technique was located between the AM and PL tunnel positions formed with the transportal DB technique. Conclusions Using the 3D-CT measuring method, the location of the tibia tunnel was between the AM and PL footprints, but the center of the femoral tunnel was at more shallow position from the AM bundle footprint when ACL reconstruction was performed by the transtibial SB technique.

Tooth loss and bone mineral density in postmenopausal South Korean women: The 2008–2010 Korea National Health and Nutrition Examination Survey

PURPOSE The current study aimed to investigate the association between the number of remaining teeth and bone mineral density (BMD) using data from the Korean National Health and Nutrition Examination Survey (KNHANES) 2008-2010. METHODS This study enrolled 7315 Korean subjects (3364 men over 50 years of age and 3951 postmenopausal women). BMD was measured using dual-energy X-ray absorptiometry at three sites: the total femur (TF), femur neck (FN), and lumbar spine (LS). The number of teeth present was categorized into four groups (≤10, 11-20, 21-25, and ≥26 teeth). RESULTS More remaining teeth were significantly associated with a higher BMD at the TF, FN, and LS in postmenopausal women after adjusting for all the covariates, but not in elderly men. In both sexes, subjects with a normal BMD tended to have more remaining teeth than those who were diagnosed with osteopenia and osteoporosis. A lower prevalence of osteoporosis was also significantly associated with more remaining teeth (number of teeth ≥26) in postmenopausal women. This trend was statistically significant in osteoporosis at the FN (p for trend=0.019). CONCLUSION The number of remaining teeth was associated with osteoporosis, especially at the FN, in postmenopausal women.