Kiattisak Duangmal

Electrochemically reduced graphene oxide-modified screen-printed carbon electrodes for a simple and highly sensitive electrochemical detection of synthetic colorants in beverages

A simple and highly sensitive electrochemical sensor based on an electrochemically reduced graphene oxide-modified screen-printed carbon electrode (ERGO-SPCE) for the simultaneous determination of sunset yellow (SY) and tartrazine (TZ) was proposed. An ERGO film was coated onto the electrode surface using a cyclic voltammetric method and then characterized by scanning electron microscopy (SEM). In 0.1M phosphate buffer at a pH of 6, the two oxidation peaks of SY and TZ appeared separately at 0.41 and 0.70V, respectively. Surprisingly, the electrochemical response remarkably increased approximately 90- and 20-fold for SY and TZ, respectively, using the modified electrode in comparison to the unmodified electrode. The calibration curves exhibited linear ranges from 0.01 to 20.0µM for SY and from 0.02 to 20.0µM for TZ. The limits of detection were found to be 0.50 and 4.50nM (at S/N=3) for SY and TZ, respectively. Furthermore, this detection platform provided very high selectivity for the measurement of both colorants. This electrochemical sensor was successfully applied to determine the amount of SY and TZ in commercial beverages. Comparison of the results obtained from this proposed method to those obtained by an in-house standard technique proved that this developed method has good agreement in terms of accuracy for practical applications. This sensor offers an inexpensive, rapid and sensitive determination. The proposed system is therefore suitable for routine analysis and should be an alternative method for the analysis of food colorants.

Mechanical, Optical, and Barrier Properties of Soy Protein Film As Affected by Phenolic Acid Addition

This study aimed to explore the effect of phenolic acid addition on properties of soy protein film. Ferulic (FE), caffeic (CA), and gallic (GA) acids as well as their oxidized products were used in this study. Phenolic acid addition was found to have a significant effect (p ≤ 0.05) on the mechanical properties of the film. GA-containing films exhibited the highest tensile strength and elongation at break, followed by those with added CA and FE, respectively. Oxidized phenolic acids were shown to produce a film with higher tensile strength and elongation at break than their unoxidized counterparts. Phenolic acid addition also affected film color and transparency. As compared to the control, phenolic-containing film samples demonstrated reduced water vapor permeability and water solubility and increased contact angle, especially at high concentrations of oxidized phenolic acid addition.

Drying of Osmosed Cantaloupe: Effect of Polyols on Drying and Water Mobility

Cantaloupe slices were sequentially immersed in 40 and 50 °Brix sucrose solutions for 24 h each. Partial replacement of the 50 °Brix sucrose solution with one of two polyhydric alcohols (sorbitol or glycerol) at 10 or 15% (w/v) was also performed along with a 0% (w/v) polyhydric alcohol treatment and a 10% (v/v) invert sugar partial replacement of the 50 °Brix solution as a control and a reference, respectively. Solids gain (SG) and water loss (WL) were determined up to 48 h later. Conversely, the treatment with 10 and 15% (w/v) sorbitol and the reference showed a significantly higher SG and a lower WL than the control (p < 0.05). The treatments with 10 and 15% (w/v) glycerol presented a significantly lower SG and higher WL than the control (p < 0.05). Increasing concentrations of polyol led to an increase in both the WL and the SG. The osmosed cantaloupe was then dried in a hot air dryer at 60°C, where the numerical drying rate was observed to be as follows: control > 10% sorbitol > 15% sorbitol > 10% glycerol > 15% glycerol > reference, but these differences at each moisture ratio were not significantly different (p > 0.05). Pages model showed a better goodness of fit with the experimental data for all treatments than did the Henderson and Pabis model. The addition of either of the two polyhydric alcohols resulted in a decreased longitudinal relaxation time (T 1), as monitored using nuclear magnetic resonance (NMR), indicating the decreased mobility of water molecules. Among all treatments evaluated, the sensory analysis derived acceptance scores for the product treated with 10% (w/v) sorbitol were not significantly different from that of the reference (p > 0.05). This may due to the ability of invert sugar and polyols to act as a humectant.

Effect of acid pretreatment and the germination period on the composition and antioxidant activity of rice bean (Vigna umbellata)

This research evaluated effect of germination period and acid pretreatment on chemical composition and antioxidant activity of rice bean sprouts. Moisture, total phenolics, reducing sugar and B vitamins (thiamine, riboflavin, and niacin) content of steamed sprouts increased with increasing germination time (p⩽0.05). Pretreatment with 1% (w/v) citric acid for 6h significantly increased the total phenolic content. The 18-h-germinated rice beans showed the highest crude protein content, as determined using the Kjeldahl method. During germination, acid pretreatment led to a significant decrease in the intensity of the 76-kDa band. Germination caused a significant increase in radical scavenging activity and ferric reducing antioxidant power, especially in sprouts from citric acid-treated seeds. The antioxidant activities of the ethanolic extracts from both pretreated beans and the control were 1.3-1.6 times higher than those obtained from the water extracts. Major phenolics found in both 0-h and 18-h-germinated rice beans were catechin and rutin.

Influence of humectants on the drying kinetics, water mobility, and moisture sorption isotherm of osmosed air-dried papaya

ABSTRACT Papaya slices were sequentially soaked in 40, 50, and 60° Brix sucrose solutions. There was a higher water loss and solids gain when 60° Brix sucrose solution was partially substituted with 10% (w/v) sorbitol, 10% (v/v) glycerol, or invert sugar. The modified Henderson and Pabis model provided the best fit to experimental drying kinetics. Moisture sorption isotherm of all dried papaya, at 30 ± 2°C, was a type III with different curvature, revealing different sugar-binding ability towards water molecules. The Henderson model showed suitable fit to experimental desorption isotherm data. NMR water mobility revealed the better ability of glycerol to bind water than sucrose.

Entrapment of 5‐aminolevulinic acid under edible composite film of konjac glucomannan and chitosan

5‐Aminolevulinic acid (5‐ALA) is a known plant regulator and growth promoter. It is a very sensitive and highly unstable compound that is easy to deteriorate. Here we propose a novel approach to stabilize 5‐ALA into a film. Films from konjac glucomannan (KGM), KGM treated with alkali solution (KGOH), chitosan (CHI) as well as blends between KGOH and CHI were fabricated for 5‐ALA entrapment. It was found that the efficiency of KGM film, KGOH film and CHI film for 5‐ALA entrapment was 55.7 ± 0.73%, 58.3 ± 0.36% and 60.3 ± 0.18 %, respectively. A 25:75 (%w/w) blended film (KGOH/CHI) showed the highest entrapment efficiency of 5‐ALA (65.9 ± 0.37%) versus other films. The possible mechanism for entrapment of 5‐ALA in blended film was postulated under two mechanisms. A secondary amide that leads to the interaction between the amino group of CHI and carboxyl group of 5‐ALA is proposed as the first mechanism. The fact that the 5‐ALA molecule was entrapped within the complexity of KGOH structure is proposed as the second mechanism. Therefore, stabilizing 5‐ALA in a film may be an alternative way to use and preserve 5‐ALA for further applications.