Kim H. Ominski

Effect of Environmental Factors and Influence of Rumen and Hindgut Biogeography on Bacterial Communities in Steers

ABSTRACT Feces from cattle production are considered important sources of bacterial contamination of food and the environment. Little is known about the combined effects of arctic temperatures and fodder tannins on rumen and hindgut bacterial populations. Individual rumen liquor and rectal fecal samples from donor steers fed either alfalfa silage or sainfoin (Onobrychis viciifolia Scop.) silage and water ad libitum were collected weekly on the first three sampling days and fortnightly afterwards. The daily ambient temperatures were registered and averaged to weekly mean temperatures. Steers fed sainfoin silage had lower (P < 0.05) concentrations of branched-chain volatile fatty acids (VFA) than those fed alfalfa silage. All VFA concentrations were higher (P < 0.001) in rumen liquor samples than in fecal samples. The interaction of sample type and diet showed a significant effect (P < 0.05) on the proportions of the bacterial community that were from the phyla Proteobacteria and Verrucomicrobia. Ambient temperature had an indirect effect (P < 0.05) on the phylum Firmicutes, as it affected its proportional balance. The bacterial population diversity in samples appeared to decrease concurrently with the ambient temperature. The phylum Firmicutes explained the first principal component at 64.83 and 42.58% of the total variance in rumen liquor and fecal samples, respectively. The sample type had a larger effect on bacterial communities than diet and temperature. Certain bacterial populations seemed to be better adapted than others to environmentally adverse conditions, such as less access time to nutrients due to higher motility and rate of passage of digesta caused by extreme temperatures, or antimicrobials such as tannins, possibly due to an influence of their biogeographical location within the gut.

Potential To Reduce Escherichia coli Shedding in Cattle Feces by Using Sainfoin (Onobrychis viciifolia) Forage, Tested In Vitro and In Vivo

ABSTRACT There is a growing concern about the presence of pathogens in cattle manure and its implications on human and environmental health. The phytochemical-rich forage sainfoin (Onobrychis viciifolia) and purified phenolics (trans-cinnamic acid, p-coumaric acid, and ferulic acid) were evaluated for their ability to reduce the viability of pathogenic Escherichia coli strains, including E. coli O157:H7. MICs were determined using purified phenolics and acetone extracts of sainfoin and alfalfa (Medicago sativa), a non-tannin-containing legume. Ground sainfoin or pure phenolics were mixed with fresh cattle feces and inoculated with a ciprofloxacin-resistant strain of E. coli, O157:H7, to assess its viability at −20°C, 5°C, or 37°C over 14 days. Forty steers were fed either a sainfoin (hay or silage) or alfalfa (hay or silage) diet over a 9-week period. In the in vitro study, the MICs for coumaric (1.2 mg/ml) and cinnamic (1.4 mg/ml) acids were 10- to 20-fold lower than the MICs for sainfoin and alfalfa extracts. In the inoculated feces, the −20°C treatment had death rates which were at least twice as high as those of the 5°C treatment, irrespective of the additive used. Sainfoin was less effective than coumaric acid in reducing E. coli O157:H7 Cipr in the inoculated feces. During the animal trial, fecal E. coli numbers declined marginally in the presence of sainfoin (silage and hay) and alfalfa silage but not in the presence of hay, indicating the presence of other phenolics in alfalfa. In conclusion, phenolic-containing forages can be used as a means of minimally reducing E. coli shedding in cattle without affecting animal production.

Comparison of techniques for estimation of forage dry matter intake by grazing beef cattle

Four techniques were compared for their ability to estimate forage dry matter intake (DMI) of grazing animals. In the Cage technique, 10 grazing cages were placed within each pasture, and forage inside and outside the cages was clipped from 0.25-m2 quadrats after 12 to 20 d of grazing. The difference between forage inside and outside the grazing cage represented forage consumed by grazing animals. The second technique used n-alkane controlled release capsules to measure DMI of individual grazing animals (N-alkane marker technique). The remaining two techniques were prediction equations; one utilized body weight (BW) and average daily gain (ADG) to estimate DMI (Minson equation) and the other related dietary net energy concentration and BW to DMI (Net Energy equation). The four methodologies were applied to a 3-yr study evaluating liquid hog manure on the productivity of grass pastures. The study utilized steers of a relatively uniform body size on six continuously grazed grass pastures. When individual an...

Targeted 16S rRNA high‐throughput sequencing to characterize microbial communities during composting of livestock mortalities

A comprehensive understanding of the microbial community is necessary to ensure a significant reduction in pathogens during the composting process.

Estimates of enteric methane emissions from cattle in Canada using the IPCC Tier-2 methodology

The objective of this study was to estimate enteric methane (CH4) emissions of the Canadian cattle population using the International Panel on Climate Change (IPCC) Tier-2 methodology. Estimates were then compared with IPCC Tier-1 methodology and data from Canadian research studies (CRS). Animal inventory data for the Canadian beef and dairy cattle herd was obtained from Statistics Canada. Information on cattle performance and feeding practices were obtained from provincial cattle specialists via a survey, as well as various published reports. Methane emissions from dairy and beef cattle in Canada for 2001 were 173 030 t yr-1 or 3.6 Mt CO2 eq. and 763 852 t yr-1 or 16.0 Mt CO2 eq., respectively, using Tier-2 methodology. Emissions for dairy cattle ranged from 708 t yr-1 in Newfoundland to 62 184 t yr-1 in Ontario. Emissions for beef cattle ranged from 191 t yr-1 in Newfoundland to 356 345 t yr-1 in Alberta. The national emission factors (kg CH4 yr-1) using IPCC Tier-2 were 73, 126, 90, 94, 40, 75, 63 and ...

Greenhouse gas emissions of Canadian beef production in 1981 as compared with 2011

The present study compared the greenhouse gas (GHG) emissions, and breeding herd and land requirements of Canadian beef production in 1981 and 2011. In the analysis, temporal and regional differences in feed types, feeding systems, cattle categories, average daily gains and carcass weights were considered. Emissions were estimated using life-cycle assessment (cradle to farm gate), based primarily on Holos, a Canadian whole-farm emissions model. In 2011, beef production in Canada required only 71% of the breeding herd (i.e. cows, bulls, calves and replacement heifers) and 76% of the land needed to produce the same amount of liveweight for slaughter as in 1981. Compared with 1981, in 2011 the same amount of slaughter weight was produced, with a 14% decline in CH4 emissions, 15% decline in N2O emissions and a 12% decline in CO2 emissions from fossil fuel use. Enteric CH4 production accounted for 73% of total GHG emissions in both years. The estimated intensity of GHG emissions per kilogram of liveweight that left the farm was 14.0 kg CO2 equivalents for 1981 and 12.0 kg CO2 equivalents for 2011, a decline of 14%. A significant reduction in GHG intensity over the past three decades occurred as a result of increased average daily gain and slaughter weight, improved reproductive efficiency, reduced time to slaughter, increased crop yields and a shift towards high-grain diets that enabled cattle to be marketed at an earlier age. Future studies are necessary to examine the impact of beef production on other sustainability metrics, including water use, air quality, biodiversity and provision of ecosystems services.

Pasture productivity, cattle productivity and metabolic status following fertilization of a grassland with liquid hog manure: A three-year study

Forage yield and quality, cattle and pasture productivity, and enteric methane (CH4) emissions were determined from grass pastures receiving liquid hog manure. Three treatments were evaluated: grass pastures receiving no manure (Control), grass pastures receiving liquid hog manure applied as a single application of 142 ± 20 kg available N ha-1 in the spring (Single) or a split application of 70 ± 6 kg available N ha-1 in both the spring and autumn (Split). Each treatment was represented by two paddocks. The study was carried out over three grazing seasons, from 2004 to 2006, with each grazing season divided into three, 28-d periods. Growing steers (338 ± 32 kg) were introduced into pastures in early summer (May-June) of each year. Standing biomass, measured using quadrats, averaged 1.2, 2.6, and 2.0 ± 0.44 t dry matter (DM) ha-1 for Control, Split and Single pastures, respectively, and was not influenced (P = 0.13) by liquid hog manure application. Liquid hog manure application caused a shift in pasture g...

Assessing the Inactivation of Mycobacterium avium subsp. paratuberculosis during Composting of Livestock Carcasses

ABSTRACT Mycobacterium avium subsp. paratuberculosis causes Johnes disease (JD) in ruminants, with substantial economic impacts on the cattle industry. Johnes disease is known for its long latency period, and difficulties in diagnosis are due to insensitivities of current detection methods. Eradication is challenging as M. avium subsp. paratuberculosis can survive for extended periods within the environment, resulting in new infections in naïve animals (W. Xu et al., J. Environ. Qual. 38:437-450, 2009). This study explored the use of a biosecure, static composting structure to inactivate M. avium subsp. paratuberculosis. Mycobacterium smegmatis was also assessed as a surrogate for M. avium subsp. paratuberculosis. Two structures were constructed to hold three cattle carcasses each. Naturally infected tissues and ground beef inoculated with laboratory-cultured M. avium subsp. paratuberculosis and M. smegmatis were placed in nylon and plastic bags to determine effects of temperature and compost environment on viability over 250 days. After removal, samples were cultured and growth of both organisms was assessed after 12 weeks. After 250 days, M. avium subsp. paratuberculosis was still detectable by PCR, while M. smegmatis was not detected after 67 days of composting. Furthermore, M. avium subsp. paratuberculosis remained viable in both implanted nylon and plastic bags over the composting period. As the compost never reached a homogenous thermophilic (55 to 65°C) state throughout each structure, an in vitro experiment was conducted to examine viability of M. avium subsp. paratuberculosis after exposure to 80°C for 90 days. Naturally infected lymph tissues were mixed with and without compost. After 90 days, M. avium subsp. paratuberculosis remained viable despite exposure to temperatures typically higher than that achieved in compost. In conclusion, it is unlikely composting can be used as a means of inactivating M. avium subsp. paratuberculosis associated with cattle mortalities.

A typological characterization of Canadian beef cattle farms based on a producer survey

Abstract: The diverse nature of beef production was captured by establishing a farm typology based on an extensive survey of 1005 Canadian farms in 2011. The survey provided information on the type of operation, cattle numbers, feed storage and management, manure management, land use, producer demographics and attitudes to risk, and technology adoption. Principal component analysis and cluster analysis were used to understand the relationships among variables and to statistically identify farm types. A total of 41 diagnostic variables from 133 survey questions were used to define 16 principal components explaining 68% of the variation. Cluster analysis yielded eight major clusters as distinct farm types. The largest number of farms (37%) was grouped as small-scale, part-time cow-calf operations. Mixed operations (crop-beef) were next most frequent (22%), followed by large cow-calf backgrounding (18%) and diversified cow-calf operations that included crop-beef mixed operations as well as off-farm activities (11%). Cow-calf operations that finished calves comprised 8% of the total farms surveyed. Extensive cow-calf backgrounding operations, large backgrounding/finishing operations, and large finishing operations represented the remaining 3% of the farms. The typology not only provides a strategy by which the Canadian beef cattle industry can be characterized, but also improves understanding of the diversity of farm management practices to help develop policies and beneficial management practices.

Estimation of carbon dioxide production and energy expenditure of grazing cattle by the sulphur hexafluoride (SF6) tracer gas technique

The purpose of the study was to explore the suitability of the sulphur hexafluoride (SF6) tracer gas technique to estimate CO2 production and energy expenditure (EE) for grazing animals on pastures typical of western Canada. During each year of a 3-yr study, CO2 emissions were collected from 60 yearling steers on grass pastures receiving one of three fertility treatments: no manure, liquid hog manure applied as a split application (74 kg available N ha-1) in the spring and fall, and a single application of liquid hog manure applied at a rate of 155 kg available N ha-1 each spring. The mean CO2 production estimate across all treatments was 5424 ± 2218 L d-1 (mean ± SD) with a range of 1099 to 11548 L d-1. When compared across three grazing periods in June, July and August, steers produced more (P < 0.05) CO2 in June than in either July or August. Metabolic body weight (BW0.75), average daily gain (ADG), standing forage biomass, and forage neutral detergent fibre (NDF) and crude protein (CP) contents explai...