Kimberly R. Kuhlman

Diversity of Microorganisms within Rock Varnish in the Whipple Mountains, California

ABSTRACT Rock varnish from Arizonas Whipple Mountains harbors a microbial community containing about 108 microorganisms g−1 of varnish. Analyses of varnish phospholipid fatty acids and rRNA gene libraries reveal a community comprised of mostly Proteobacteria but also including Actinobacteria, eukaryota, and a few members of the Archaea. Rock varnish represents a significant niche for microbial colonization.

Planetary Imaging in Powers of Ten: A Multiscale, Multipurpose Astrobiological Imager

Contextual, multiscale astrobiological imaging is necessary to discover, map, and image patchy microbial colonization in extreme environments on planetary surfaces. The large difference in scale--several orders of magnitude--between search environment and microorganisms or microbial communities represents a challenge, which to date no single imaging instrument is able to overcome. In support of future planetary reconnaissance missions, we introduce an adapter-based imager, built from an off-the-shelf consumer digital camera, that offers scalable imaging ranging from macroscopic (meters per pixel) to microscopic (micrometers per pixel) imaging, that is, spanning at least 6 orders of magnitude. Magnification in digital cameras is governed by (1) the native resolution of the CCD/CMOS chip of the camera, (2) the distance between camera and object to be imaged (focal length), and (3) the built-in optical and digital zoom. Both telezoom and macro mode alone are usually insufficient for microscopic imaging. Therefore, the focal distance has to be shortened, and the native CCD resolution of the camera has to be increased to attain a microscopic imaging capability. Our adapter-based imager bridges the gap between macroscopic and microscopic imaging, thereby enabling for the first time contextual astrobiological imaging with the same instrument. Real-world applications for astrobiology and planetary geology are discussed, and proof-of-concept imagery taken with our prototype is presented.

Tumbleweed: A New Paradigm for Surveying the Surface of Mars for In-Situ Resources

Mars missions to date have interrogated the planet at very large scales using orbital platforms or at very small scales intensively studying relatively small patches of terrain. In order to facilitate discovery and eventual utilization of Martian resources for future missions, a strategy that will bridge these scales and allow assessment of large areas of Mars in pursuit of a resource base will be essential. Long-range surveys of in-situ resources on the surface of Mars could be readily accomplished with a fleet of Tumbleweeds - vehicles capable of using the readily available Martian wind to traverse the surface of Mars with minimal power, while optimizing their capabilities to perform a variety of measurements over relatively large swaths of terrain. These low-cost vehicles fill the niche between orbital reconnaissance and landed rovers, which are capable of much more localized study. Fleets of Tumbleweed vehicles could be used to conduct long-range, randomized surveys with simple, low-cost instrumentation functionally equivalent to conventional coordinate grid sampling. Gradients of many potential volatile resources (e.g. H2O, CH4, etc.) will also tend to follow wind-borne trajectories thus making the mobility mode of the vehicles well matched to the possible target resources. These vehicles can be suitably instrumented for surface and near-surface interrogation and released to roam for the duration of a season or longer, possibly on the residual ice cap or anywhere orbital surveillance indicates that usable resources may exist. Specific instrument selections can service the exact exploration goals of particular survey missions. Many of the desired instruments for resource discovery are currently under development for in-situ applications, but have not yet been miniaturized to the point where they can be integrated into Tumbleweeds. It is anticipated that within a few years, instruments such as gas chromatograph mass spectrometers (GC-MS) and ground-penetrating radar (GPR) will be deployable on Tumbleweed vehicles. The wind-driven strategy conforms to potential natural gradients of moisture and potentially relevant resource gases that also respond to wind vectors. This approach is also useful for characterizing other resources and performing a variety of basic science missions. Inflatable and deployable structure Tumbleweeds are wind-propelled long-range vehicles based on well-developed and field tested technology (Antol et al., 2005; Behar et al., 2004; Carsey et al., 2004; Jones and Yavrouian, 1997; Wilson et al., 2008). Different Tumbleweed configurations can provide the capability to operate in varying terrains and accommodate a wide range of instrument packages making them suitable for autonomous surveys for in-situ natural resources. Tumbleweeds are lightweight and relatively inexpensive, making them very attractive for multiple deployments or piggybacking on larger missions.

Helium Isotopes in the Lunar Regolith – Measuring Helium Isotope Diffusivity in Lunar Analogs

The purpose of the present chapter is to provide information about the diffusivities and activation energies of 4He and 3He in analogs of lunar ilmenite, which has been theorized to retain helium isotopes better than other lunar minerals. The presence of noble gases in the surfaces of lunar fines was discovered in early Apollo samples by several investigations (Bauer et al. 1972, Ebergart et al. 1970, Hintenberger et al. 1970). The correlation between lunar helium and the mineral ilmenite was discovered by Eberhart et al. (1970) and subsequently others (Muller et al. 1976, Signer et al. 1977). This correlation was rediscovered by researchers at the University of Wisconsin in the early 1980’s while researching sources of 3He for fuel for future fusion reactors (Cameron 1988). The data presented here raise questions about the diffusivities of helium in other lunar minerals and will be useful in the design of future lunar miners. This chapter also provides insight into the mechanisms of space weathering on airless bodies.