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Featured researches published by Kimihiko Matsuzawa.


British Journal of Haematology | 1994

Determination of plasma tissue factor antigen and its clinical significance

Takatoshi Koyama; Kenji Nishida; Shinichi Ohdama; Megumi Sawada; Naomi Murakami; Shinsaku Hirosawa; Renjiro Kuriyama; Kimihiko Matsuzawa; Ryoichi Hasegawa; Nobuo Aoki

SUMMARY. To investigate the clinical significance of determination of plasma tissue factor (TF) antigen, we have developed a highly sensitive enzyme‐linked immunosorbent assay (ELISA) for plasma TF, using two different monoclonal antibodies against TF apoprotein, 6B4 (catching antibody) and 5G9 (detecting antibody), and tetramethyl benzidine/H2O2 as substrates. Titration curves of recombinant human TF in buffer containing Triton X‐100 were linear within the range from 50 to 2000pg/ml. The total assay time was 3h. Ultracentrifugation and immunoblot analysis indicated that human plasma and urine contained 50 000g sedimentable and non‐sedimentable forms of TF, both of which were detected by our ELISA method.


Archive | 1985

Permselective hollow fiber membrane, process for the preparation thereof, method and apparatus for plasma components separation

Eiichi Murakami; Kimihiko Matsuzawa; Eiji Masunaga


Archive | 1975

Novel immunoglobulin derivatives and process for the preparation thereof

Katsuhiko Tomibe; Yasuhiko Masuho; Kimihiko Matsuzawa; Sachio Ishimoto; Kazuo Satake; Tsuneo Watanabe


Archive | 1993

Method for determining chondrocalcin

Kimihiko Matsuzawa; Kazuhiko Itou; Hitomi Honda; Ryoichi Hasegwwa; Naomi Okamoto; Kenji Hosoda; Masayuki Shinmei; Shigeo Matsuyama


Archive | 1985

Permselective hollow yarn membrane, method of producing the same, method of separating plasma components, and plasma component separator

Eiichi Murakami; Kimihiko Matsuzawa; Eiji Masunaga


Archive | 1990

Immunoassay of human thrombomodulin, and reagent and kit therefor

Kimihiko Matsuzawa; Ryoichi Hasegawa


Archive | 1983

Permselective hollow yarn membrane, serum component separation method using same, serum separator, serum component separating apparatus and blood treating apparatus

Eiichi Murakami; Kimihiko Matsuzawa


Journal of Biochemistry | 1976

Reconstruction of intact gamma-globulin from S-sulfonated gamma-globulin in vivo.

Yasuhiko Masuho; Katsuhiko Tomibe; Kimihiko Matsuzawa; Tsuneo Watanabe; Sachio Ishimoto


Archive | 1992

Method for measurement of tissue factor in high sensitivity and measurement kit therefor

Kimihiko Matsuzawa; Ryoichi Hasegawa


Archive | 1992

Highly sensitive assay of tissue factor and kit therefor

Kimihiko Matsuzawa; Ryoichi Hasegawa

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