Kiminobu Takeda
Kanazawa Medical University
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Featured researches published by Kiminobu Takeda.
International Journal of Dermatology | 2005
Takashi Mochizuki; Kiminobu Takeda; Masato Nakagawa; Masako Kawasaki; Hiroshi Tanabe; Hiroshi Ishizaki
A 26‐year‐old woman with no family history of dermatophytosis presented with pruritic erythema on the right palm ( Fig. 1 ) in November 2002, 1 month after first noticing it. She had no lesions on the soles, toe webs, left palm, or nails. The erythema had been treated with topical steroids for 4 weeks, but had continued to expand. The erythema measured 42 mm × 36 mm, and was hyperemic and infiltrated unevenly. Its center showed no signs of healing, its periphery exhibited small vesicles, and its margin showed scales. On the scales, fungal hyphae and chains of arthroconidia were revealed by direct KOH test. The erythema was diagnosed as tinea manuum and was treated with topical terbinafine once daily. After several days of treatment, the erythema flared up and red papuloerythemas appeared on the back of both hands and on both forearms. Ten days after the first visit, the patient returned to our clinic for the treatment of newly developed eruptions. A trichophytin skin test produced an infiltrated erythema measuring 21 mm × 18 mm, confirming that the newly developed eruptions and exacerbated erythema on the right palm were due to a trichophytid reaction. Topical terbinafine was continued for the palm and fluocinolone acetonide ointment was applied on the newly developed eruptions on the arms from day 10 after the first visit. All the inflammatory eruptions subsided after 4 weeks of treatment and direct KOH test results were negative.
Mycopathologia | 2017
Takashi Mochizuki; Kiminobu Takeda; Kazushi Anzawa
Dermatophytosis is a very common skin disorder and the most frequent infection encountered by practicing dermatologists. The identification, pathogenicity, biology, and epidemiology of dermatophytes, the causative agents of dermatophytosis, are of interest for both dermatologists and medical mycologists. Recent advances in molecular methods have provided new techniques for identifying dermatophytes, including intraspecies variations. Intraspecies subtyping and strain differentiation have made possible the tracking of infections, the identification of common sources of infections, recurrence or reinfection after treatment, and analysis of strain virulence and drug resistance. This review describes molecular methods of intraspecies subtyping and strain differentiation, including analyses of mitochondrial DNA and non-transcribed spacer regions of ribosomal RNA genes, random amplification of polymorphic DNA, and microsatellite markers, along with their advantages and limitations.
Medical mycology journal | 2016
Kiminobu Takeda; Hirokazu Mochizuki; Katsuhiko Izumi; Yuichi Sakata; Tsuyoshi Ushigami; Akiko Nishibu; Kazushi Anzawa; Takashi Mochizuki
We cultured 15 isolates of Trichophyton rubrum and one isolate of Trichophyton mentagrophytes from an 82-year-old male tinea patient with multiple lesions. To determine whether feet lesions were the source of dermatophytes of other tinea lesions, we extracted total cellular DNA from the T. rubrum isolates(13 from feet, two from right waist and buttock). PCR targeting the non-transcribed spacer(NTS)region of ribosomal RNA gene was performed. Molecular polymorphisms were detected by length variation of amplicons.Four molecular types were found among the 15 isolates. The predominant type, which we previously named Type III, comprised seven isolates cultured from both feet and from left waist and buttock. This was followed by Type VI, five isolates; Type V, two isolates; and Type IV, one isolate. Apart from type III, which was cultured from both feet, isolates were cultured from one foot only. The patient was successfully treated for all types with a six-month course of oral terbinafine and topical luliconazole. The molecular typing supported the notion that tinea pedis was the source of tinea corporis in the patient.
Journal of Dermatology | 2015
Takashi Mochizuki; Kiminobu Takeda; Kazushi Anzawa
Dermatophytosis is a very common skin disorder and the most prevalent infectious disease treated by dermatologists. Recent developments in molecular techniques have markedly changed methods of identifying dermatophytes, with these methods showing intraspecies polymorphisms in some molecular markers. Intraspecies subtyping and strain differentiation have made possible the tracking of infections, the identification of common sources of infections and recurrence or reinfection after treatment. This review describes methods of intraspecies differentiation using mitochondrial DNA, random amplification of polymorphic DNA, non‐transcribed spacer regions of ribosomal RNA genes and microsatellite markers, as well as their usefulness and limitations.
Journal of Dermatology | 2013
Maki Hasei; Kiminobu Takeda; Kazushi Anzawa; Akiko Nishibu; Hiroshi Tanabe; Takashi Mochizuki
A 90‐year‐old Japanese woman, taking prednisolone (5–10 mg/day) for polyarthritis, presented to our hospital with multiple subcutaneous lesions on her left arm in 2009. Her history included excision of a phaeomycotic cyst on the left middle finger in 2007. There were three subcutaneous nodules approximately 15 mm in diameter around her left wrist and a large soft cystic lesion measuring 80 mm × 60 mm on her left elbow. A granuloma with neutrophilic infiltration was detected in the deep dermis of a biopsy specimen. Chains composed of round brown cells and short pseudomycelia were found in the granuloma. Fungal cultures from the samples confirmed Exophiala sp. to be the causative agent. Treatment with terbinafine and local hyperthermia seemed effective as all the lesions tended to subside. However, the patient died due to pneumonia approximately 1 month after commencement of therapy.
Journal of Dermatology | 2010
Kiminobu Takeda; Jun Fujita; Toshiki Fujii; Hiroshi Tanabe; Takashi Mochizuki; Makoto Yanagihara
A 73‐year‐old woman developed linear erythema at the sites of scratching‐induced scars on the bilateral thighs 2 weeks before the initial consultation. Subsequently, edematous erythema developed in the upper eyelids, dorsum of the nose and the face, and pitting edema in the dorsum of the bilateral hands and feet. The C‐reactive protein (CRP) level was 8.2 mg/dL and erythrocyte sedimentation rate (ESR) 121 mm/h. The antinuclear antibody titer was 1:160, and rheumatoid factor (RF) and anti‐Jo‐1 antibody were negative. X‐ray examination of the bilateral hands showed neither narrowing of the joint spaces nor bone erosion. Ga scintigraphy showed synovitis of the bilateral wrists. A diagnosis of remitting seronegative symmetrical synovitis with pitting edema syndrome (RS3PE) was made. The erythema disappeared after diclofenac sodium administration. However, because the joint swelling and pitting edema did not improve, p.o. administration of prednisolone (20 mg/day) was initiated. The CRP and ESR levels normalized 2 months after the initiation of administration, and pitting edema disappeared after 3 months. We report this case because linear erythema like that observed in dermatomyositis has not been described as eruptions associated with RS3PE.
Journal of Dermatology | 2018
Kiminobu Takeda; Kazushi Anzawa; Takashi Mochizuki; Shigeo Yamada; Hiroto Kobayashi; Satoru Kimura
Dear Editor, Microsporum canis, a zoophilic dermatophyte species usually parasitic or saprophytic on small pet animals such as cats and dogs, sometimes causes tinea corporis and tinea capitis in women and children who interact with infected animals. However, the infection is seldom reported in neonates and infants. Recently, we treated an infant with tinea faciei caused by M. canis, which a molecular study suggested was contracted from pet animals. The mother of a 2-month old boy noticed a red skin lesion around his left eye from 1 month after birth, and topical application of prednisolone valerate acetate ointment worsened the eruption. At the first consultation, demarked erythema was observed on the left side of his nose, glabella, and upper and lower eyelids, with the eyelashes caked with wet scales (Fig. 1a). During cleaning of the lesion, some trapped eyelashes were detached with the scales which showed positive for fungus by direct KOH examination (Fig. 1b). A fungal culture was positive for M. canis (Fig. 1c). The mother and son had lived at his grandparents’ residence for 4 weeks, where two cats were kept as pets. However, the mother said the neonate had no occasion to have direct physical contact with the animals. The mother had tinea pedis but fungal culture failed. No other family member had complained of tinea. As treatment, white petrolatum then terbinafine cream were applied once daily and the erythema and scale disappeared after 4 weeks but fungal elements were revealed on his eyelashes by direct KOH examination. After 6 weeks, his eyelashes had regrown and the treatment was terminated. Some hairs of the two cats (6-year-old Russian Blue and 2-year-old Somali) were pulled and collected for examination. The Somali had been considered the source of the infection because this cat was molting, but both cats were positive for fungal cultures. These three strains of M. canis (KMU7067–9) were genotyped by microsatellite (MS) profiles using six primer pairs named MS1, 2 and 4–7, which is the most sensitive method for genotyping of M. canis. As a result, all three isolates were classified as type A which we designated previously. We classified 70 Japanese clinical isolates into 20 genotypes A–T with A being the most prevalent (21.4%), and also found in each five different episodes of infection among humans and pet cats. Following the results of our genotyping of the fungal cultures from the boy and pets, the family agreed to segregate their living space in the residence and to consult a veterinarian for removal of the fungus from the cats. Thus, intraspecies genotyping is a useful method for analyzing and tracing fungal infections. Treatment of dermatophytoses often involves application of topical antifungals but there is sometimes a high risk of recurrence in cases involving hair. Although eyelashes were infected with M. canis in our case, topical terbinafine induced sufficient resolution, otherwise liquid itraconazole would have been the only usable therapeutic agent.
Nippon Ishinkin Gakkai Zasshi | 2008
Masako Kawasaki; Kazushi Anzawa; Asako Wakasa; Kiminobu Takeda; Hiroshi Tanabe; Takashi Mochizuki; Hiroshi Ishizaki; Basavaraj M.Hemashettar
Japanese Journal of Infectious Diseases | 2012
Kiminobu Takeda; Akiko Nishibu; Kazushi Anzawa; Takashi Mochizuki
Japanese Journal of Infectious Diseases | 2008
Takashi Mochizuki; Masako Kawasaki; Kazushi Anzawa; Jun Fujita; Tsuyoshi Ushigami; Kiminobu Takeda; Ayako Sano; Yoko Takahashi; Katsuhiko Kamei