Kin-Ya Sohda

YM-216391, a Novel Cytotoxic Cyclic Peptide from Streptomyces nobilis : II. Physico-chemical Properties and Structure Elucidation

AbstractYM-216391, a novel cytotoxic cyclic peptide, has been isolated from the cultured mycelium of Streptomyces nobilis JCM 4274. The planar structure of YM-216391 was assigned on the basis of 1D and 2D NMR spectroscopic techniques. The absolute configuration of the amino acid residues in YM-216391 was determined by Marfeys analysis and chiral HPLC analysis of its acid hydrolysate.

YM-216391, a novel cytotoxic cyclic peptide from Streptomyces nobilis. I. fermentation, isolation and biological activities.

AbstractYM-216391, a novel cyclic peptide, was isolated from the cultured mycelium of Streptomyces nobilis JCM 4274. It was purified by solvent extraction, silica gel and ODS flash column chromatographies, followed by preparative HPLC. YM-216391 dose-dependently inhibited the growth of human cervical cancer HeLa S3 cells with an IC50 value of 14 nM. YM-216391 also showed potent cytotoxic activity against a human cancer cell line panel.