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Kinjiro Sukegawa

Obihiro University of Agriculture and Veterinary Medicine

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Nihon Chikusan Gakkaiho | 1988

Effect of Carbethoxylation of Histidyl Residues in Bovine IgG2 on the Interaction with Staphylococcal Protein A

Kei-ichi Shimazaki; Tetsuya Tsuchimoto; Kinjiro Sukegawa

血液中および乳汁中に含まれている免疫グロブリンと,Staphylococcus aureusの産生するプロテインAとの相互作用は,免疫グロブリンの各クラス,サブクラス間でさらには動物種間でその様式は微妙に異なっている.ウシ免疫グロブリンのIgGクラスでは,IgG 1はプロテインAと相互作用せず,IgG 2が相互作用をする事がプロテインA-セファローズCL-4 Bを用いたアフィニティークロマトグラフィーで確かあられた.さらにIgG 2のヒスチジン残基をジエチルピロカルボネートによりエトキシホルミル化したところ,4.2-8.3個のヒスチジン残基が修飾され,かつ7.7個以上修飾されたIgG 2ではプロテインA-セファローズカラムへの結合能は全く見られなくなった.ヒドロキシアミンにより修飾基を脱離すると,プロテインA-セファローズカラムへの結合能が回復し,ウシIgG 2とプロテインAとの相互作用にIgG 2のヒスチジン残基の寄与が不可欠である事が示された.

Journal of Dairy Science | 1982

Chromatographic Profiles of Bovine Milk Whey Components by Gel Filtration on Fractogel TSK HW55F Column

Kei-ichi Shimazaki; Kinjiro Sukegawa

Agricultural and biological chemistry | 1983

Analysis of Bovine Whey Protein Components by Two-dimensional Electrophoresis

Kei-ichi Shimazaki; Shunsuke Tezima; Kinjiro Sukegawa

Nihon Chikusan Gakkaiho | 1978

Orotic Acid Concentration in Bovine Normal, Colostral and Mastitic Milk

Kei-ichi Shimazaki; Masato Sasaki; Kinjiro Sukegawa

Nihon Chikusan Gakkaiho | 1987

Susceptibility of Bovine Colostral and Serum lgG to Proteolytic Enzymes as Analyzed via Gel Filtration Chromatographic Behavior

Kei-ichi Shimazaki; Yoshiyuki Maki; Kinjiro Sukegawa

Journal of Food Science and Technology-mysore | 1984

Reduction of Nitrate and Nitrite in Cheese and Nitrate Reducibility of Penicillium candidum as Fungal Starter

Hideko Ariga; Tsutomu Ohtsuka; Satoshi Hattori; Takumi Ohnishi; Kinjiro Sukegawa

Nippon Eiyo Shokuryo Gakkaishi | 1981

Comparison of Nutritional Changes between UHT-Pasteurized Milk and UHT-Sterilized Milk

Kinjiro Sukegawa; Hisashi Murasawa; Masato Sasaki

Nihon Chikusan Gakkaiho | 1981

Comparison of Binding Amounts of Bovine Immunoglobulin G1 and G2 to Staphylococcus aureus

Kei-ichi Shimazaki; Kinjiro Sukegawa

Nihon Chikusan Gakkaiho | 1981

Agglutinating Activities of Bovine lmmunoglobulins from Mastitic Milk against Staphylococci

Kei-ichi Shimazaki; Hidetoshi Aoyama; Kinjiro Sukegawa

Agricultural and biological chemistry | 1981

Kinetic Analysis of Reactivity of Amino Groups in Bovine Secretory IgA with 2, 4, 6-Trinitrobenzenesulfonic Acid

Kei-ichi Shimazaki; Kinjiro Sukegawa

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Kei-ichi Shimazaki

Obihiro University of Agriculture and Veterinary Medicine

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Hideko Ariga

Obihiro University of Agriculture and Veterinary Medicine

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Satoshi Hattori

Obihiro University of Agriculture and Veterinary Medicine

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Shunsuke Tezima

Obihiro University of Agriculture and Veterinary Medicine

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Takumi Ohnishi

Obihiro University of Agriculture and Veterinary Medicine

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Tsutomu Ohtsuka

Obihiro University of Agriculture and Veterinary Medicine

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Yoshiyuki Maki

Obihiro University of Agriculture and Veterinary Medicine

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