ita K

A novel Ph1 chromosome positive cell line established from a patient with chronic myelogenous leukemia in blastic crisis

A novel Philadelphia (Ph1) chromosome positive cell line, designated KYO-1, was established from the peripheral blood of a patient with chronic myelogenous leukemia (CML) in blastic crisis. Although this line had a unique capacity to differentiate spontaneously along the erythroid and monocytoid lineages as evidenced by cytochemical analysis for the first several months, the capacity was gradually lost after repeated passages. The results suggest that KYO-1 is an undifferentiated myeloid cell line. This cell line provides a useful source for studying differentiation and proliferation of pluripotent stem cells from CML in blastic crisis.

Acute leukemia with two cell populations of lymphoblasts and monoblasts

A 46-year-old man had acute leukemia with two cell populations of lymphoblasts and monoblasts (L1 and M5-b in FAB classification, respectively), which were characterized by morphological, cytochemical and cell marker studies. At the time of diagnosis about 80% blasts were terminal deoxynucleotidyl transferase (TdT) positive lymphoid cells, while the rest were TdT negative monocytoid cells. After induction chemotherapy of vindesine and prednisolone for 15 days, almost all blasts were TdT negative monocytoid cells. Therefore, an additional course of the chemotherapy with the protocol for acute nonlymphocytic leukemia was given and one month later the patient achieved complete remission.

Sinus histiocytosis with massive lymphadenopathy in a Japanese male with symptoms of dyspnea and visual disturbance.

A case study of sinus histiocytosis with massive lymphadenopathy is reported. A Japanese male developed lymphadenopathy in the nasal cavity 10 years ago. Biopsy specimens demonstrated massive histiocytosis with phagocytosis of lymphocytes, plasmacytes, and erythrocytes. During the course of his illness, the patient experienced an episode of massive nasal bleeding, and a tracheotomy was performed because of dyspnea. He had also an operation by Killians approach to relieve a visual disturbance. The patient has been treated with cyclophosphamide and prednisolone for 6 months.