Kıvılcım Sönmez

Pathologic Alterations Detected in Acute Pancreatitis Induced by Sodium Taurocholate in Rats and Therapeutic Effects of Curcumin, Ciprofloxacin and Metronidazole Combination

Background and Aims: Secondary bacterial infections and free radical injury have been known to play an important role in the pathogenesis and clinical outcome of acute pancreatitis. Despite the therapy models developed in recent years, the mortality rate is still reported to be higher than expected. The objective of this study therefore was to investigate the effectiveness of ciprofloxacin and metronidazole combination and curcumin together in the treatment of acute pancreatitis. Methods: Acute pancreatitis was induced in rats by sodium taurocholate (n = 60). Starting 6 h after the induction of acute pancreatitis, groups I and II were injected 200 mg/kg ciprofloxacin and 500 mg/kg metronidazole intraperitoneally every 12 h for 6 days. Groups II and III received 100 mg/kg curcumin since day 20 prior to the initiation of acute pancreatitis. On day 6, animals of all groups were killed. Blood and tissue samples were taken for biochemical, pathologic and bacteriologic examination. Results: No statistical difference in the treatment groups versus the non-treatment group has been detected in the pancreatic tissue on the basis of histopathological scoring results. Prevalences of bacterial translocation were significantly lower in the treatment groups (groups I–III) than in the non-treatment group (group IV) (p < 0.001, p < 0.001, p < 0.05, respectively). Serum amylase, lipase, malon dialdehyde and nitric oxide (except for nitric oxide level in group I), levels of groups I, II and III were significantly lower than those of group IV (p < 0.05). Conclusions: The administration of ciprofloxacin and metronidazole in combination and curcumin in acute pancreatitis failed to provide a preventive effect on the occurrence of tissue injury, whereas free radical injury and prevalence of bacterial translocation were reduced significantly.

Bir Köpekte Ovarian Granüloza Hücre Tümörü ve Kompleks Meme Adenokarsinomu

On bir yasli boxer kopek rutin kisirlastirma icin Dogum ve Jinekoloji Anabilim Dali klinigine getirildi ve histopatolojik olarak granuloza hucre tumoru (GHT) olarak teshis edilen bir kitle tesadufen saptandi. Ameliyat esnasinda hormonal analiz icin kan ornegi toplandi ve bu kan orneginde ostrojen konsantrasyonu referans degerlerin altinda, progesteron konsantrasyonu ise normal olarak tespit edildi. Kopegin ilk muayenesi esnasinda meme bezlerinde genislemeler tespit edildi ve herhangi bir vulvar akinti/sislik tespit edilmedi. Kisirlastirmayi takiben bir ay sonra kopek total bilateral mastektomi icin klinigimize tekrar getirildi. Histopatolojik olarak tumorel kitleler kompleks adenokarsinom tip II olarak tespit edildi. Kopekte hem GHT hem de meme tumoru ayni anda bulundugu halde, GHT’nun kopek meme tumoru gelisimi uzerine etkisi tam anlamiyla anlasilamadi. Serum ostrojen ve progesteron konsantrasyonlarinin referans degerlerden yuksek olmamasina bagli olarak, GHT’nun henuz hormon salgilamadigi dusunuldu. Sonuc olarak, bu olgu GHT’nun kopek meme tumorunun gelisimi uzerine olasi etkisinden bahsetmesinden ve kopekte meme tumoru varliginda bu hastaligin goz ardi edilmemesi gerekliligini onermesinden dolayi onemlidir.

Investigation of human papillomaviruses (HPV), mouse mammary tumour virus (MMTV), Epstein–Barr virus (EBV), and human polyomavirus entities in canine mammary tumours

Abstract Introduction: The aim of the study was to investigate the presence of human papillomaviruses (HPV), mouse mammary tumour virus (MMTV), Epstein-Barr virus (EBV), and human polyomavirus BK in canine mammary tumours (CMTs) and to correlate the results of histopathological classification with the results of virological examination. Material and Methods: Eighty CMTs and ten normal canine mammary gland samples were evaluated using histopathological methods and TaqMan real-time PCR analysis. Results: The results indicated that all mammary tumours and normal mammary tissue samples were negative for HPV16 and other HPV, EBV, human polyomavirus, and human mammary tumour virus strains. Conclusion: Further studies should be performed to investigate the existence of other strains of HPV, EBV, and human polyomavirus in CMTs.

Immunohistochemical detection of tumour cell proliferation and intratumoural microvessel density in canine malignant mammary tumours

Abstract The objective of this study was to investigate the correlation between different histological types and grades of canine malignant mammary tumours, tumour cell proliferation and their angiogenic activity using immunohistochemical markers. Mammary tissue samples from 47 bitches with mammary cancer were evaluated. The expression of cellular proliferation marker Ki-67 and endothelial marker Von Willebrand’s factor (vWF) were immunohistochemically demonstrated. The tumours with the highest Ki-67 and vWF expressions were found to share similar histomorphological features. Simple solid carcinoma had the highest levels of Ki-67, vWF, and higher histological grade while complex carcinomas, osteosarcomas, and carcinosarcomas had the lowest ones. The differences between the expressions of Ki-67 and vWF in different tumour types were considered to be of great importance in determination of biological behaviour and prognosis of these tumours. This study is one of the few studies that evaluate these differences among the subtypes of malignant canine mammary tumours