Kiyoshi Matsukawa

The Screening of Cattle with Potential for Developing Leukemia by Using Monoclonal Antibody against Bovine Leukemia Cells

Tumor cells from cattle with enzootic bovine lymphosarcoma (EBL) have a tumor‐associated antigen (TAA) which is distinct from bovine leukemia virus (BLV)‐induced antigens. We were able to sacrifice 8 TAA‐positive cattle with no clinical signs of EBL and to examine whether or not they had gross or histological tumors. At necropsy, 4 animals had tumors macroscopically. Three animals had no tumors histologically but had initial lesions showing follicular hyperplasia and had the TAA on affected lymph nodes. The remaining one showed medullary hyperplasia in the spleen but there were no findings of tumors. These results suggest that most BLV‐infected cattle which are TAA‐positive but have no clinical signs of EBL, do have tumors and have a higher potential for developing EBL in the future when compared to BLV‐infected but TAA‐negative cattle.

C cell hyperplasia and carcinoma developing in sheep with experimentally-induced lymphosarcoma

Two cases of C cell hyperplasia and one case of C cell carcinoma of the thyroid glands were bilaterally recognized in 11 sheep with experimentally-induced lymphosarcoma. The serum calcium concentration in the C cell carcinoma case was slightly increased above the normal concentration of around 9 mg per dl. Bilateral C cell hyperplasia also developed in the thyroid lobes of the C cell carcinoma case. Immunohistochemically, hyperplastic C cells and tumour cells were positive for calcitonin, calcitonin gene-related peptide, chromogranin A and neurone-specific enolase. No amyloid deposition nor multiple endocrine neoplasia was demonstrated in any of the cases. Ultrastructurally, many secretory granules were observed in the cytoplasm of neoplastic cells constituting the C cell carcinoma and in the hyperplastic C cells.

Histochemistry of glandular metaplasia at the trigone of the urinary bladder in cows

The epithelium at the trigone of the urinary bladder showed intestinal metaplasia of a colonic type in three Holstein-Friesian cows affected with chronic polypoid cystitis. Except for Ricinus communis (RCA-I), almost all goblet cells in the whole crypt were positive to periodic acid-Schiff (PAS), alcian blue (AB) pH 2.5, AB pH 1.0, periodate borohydride-potassium hydroxide-PAS (PB-KOH-PAS), Ulex europaeus (UEA-I), Triticum vulgare (WGA) and Arachis hypogaea (PNA) after neuraminidase digestion. This result indicated that most goblet cells contained acidic and neutral glycoconjugates as O-acetylated sialomucin, L-fucose, N-acetyl-glucosamine, neuraminic acid residues and sialic acid-galactose dimers and were devoid of beta-D-galactose. The goblet cells at the surface in the upper half of the crypt contained both sulpho- and sialo-mucins with N-acetyl-galactosamine residues by AB (pH 2.5)-PAS, high iron diamine (HID)-AB (pH 2.5), Dolichos biflorus (DBA) and Glycine maximus (SBA) reactions. On the other hand, the lower goblet cells were found to contain predominantly sulphated mucins with D-mannose and D-glucose residues by AB-PAS, HID-AB and Concanavalia ensiformis (Con A) reactivities. This suggested that mucin secreted from these cells was similar to that secreted from the goblet cells of the large intestine in cattle.

A Case of Canine Chondrosarcoma Observed on the Thoracic Vertebra

脊椎孔内に侵入増殖した腫瘍により脊髄の圧迫, 変形がみられ, 後躯麻痺を呈した10歳の雄シェパードの第11胸椎から第12胸椎左側に認められた軟骨肉腫について病理学的検索を行なった. 腫瘍組織は, 鶏卵大, 表面は軟骨膜で被われていた. 割面は, 灰青色, 透明感あり, 小葉構造を示した. 腫瘍組織は, 胸椎において, 椎体および椎弓に浸潤増殖し, さらに, 左側稚間孔より椎孔内ヘ侵入し, 髄膜および胸髄を右側ヘ三日月状に圧迫していた. 胸髄の神経細胞および神経線維の変性, 左側胸髄神経背および腹根の神経線維の変性, 硬膜および軟膜の肥厚が認められた. 腫瘍実質は, 淡明均質なる基質と, 大小の小腔内におさまった種々の形態を示す異形性軟骨細胞から構成されていた. 腫瘍細胞は, 円形もしくは楕円形の大型淡明核, クロマチンが濃染する二核, もしくは多核性巨大核などを有していた. 周囲筋組織に対しては, 圧排性に, 骨組織に対しては, 浸潤性, 融合性に増殖していた. 原発巣ほ, 第11胸椎肋椎関節部と思われた. 多発した変形性脊椎症が該部に異常な力学的負担を加える原因となり, それが腫瘍形成に関与したのかもしれない. 脊椎原発の軟骨肉腫についての文献的考察にもとづき, その報告例は, 極めで少なく, 興味ある1例と考えられた. 謝辞: 稿を終えるにあたり, 本論文の御校閲をいただいた岩手大学農学部 大島寛一教授に心から感謝の意を表します. 又, 検索の機会を与えて下さった酪農学園大学獣医学科 高橋清志助教授ならびに小谷忠生助教授に対し謝意を表します.