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Publication
Featured researches published by Kiyoshi Tominaga.
Journal of Infection and Chemotherapy | 2015
Tadayoshi Ikebe; Kazuki Chiba; Tomoko Shima; Chieko Masuda; Rumi Okuno; Hitomi Ohya; Kikuyo Ogata; Chihiro Katsukawa; Ryuji Kawahara; Kiyoshi Tominaga; Junko Yabata; Yuki Tada; Nobuhiko Okabe; Haruo Watanabe; Bin Chang; Michinaga Ogawa; Makoto Ohnishi
Infection with Streptococcus agalactiae has long been recognized in infants. In recent years, S. agalactiae is an important cause of morbidity and mortality among adults and among those with underlying medical condition. Several cases of GBS infection and more fulminant disease similar to streptococcal toxic shock syndrome have recently been reported. We report here that 19 S. agalactiae strains were isolated from streptococcal toxic shock-like syndrome cases involving adult patients in Japan between 2009 and 2013. The average age of the patients was 66.3 years. At least one underlying disease was present in 47.4% (9/19) of the patients. The most prevalent serotype among these strains was Ib. All serotype Ib strains belonged to clonal complex 10 and were ciprofloxacin resistant. In contrast, all strains were susceptible to penicillin G, ampicillin, cefazolin, cefotaxime, imipenem, panipenem, and linezolid. The characteristic type distributions of streptococcal toxic shock-like syndrome isolates differed between isolates obtained from vaginal swabs of women and infants with invasive infections.
Japanese Journal of Infectious Diseases | 2015
Mitsuhiro Kameyama; Junko Yabata; Yasuharu Nomura; Kiyoshi Tominaga
The biochemical features and virulence gene profiles of 37 enterohemorrhagic Escherichia coli (EHEC) strains belonging to serogroups other than O157 and O26 (non-O157/O26 EHEC) were investigated. All strains were isolated from humans between 2002 and 2013 in the Yamaguchi Prefecture. Serogroup O111 strains were the most common, followed by O103, O121, and O145. Most strains (84%) were negative for sorbose fermentation, whereas only 1 and 2 were negative for sorbitol and rhamnose fermentation, respectively. Two strains lacked β-D-glucuronidase activity. Shiga toxin (stx) subtyping revealed 6 genotypes:stx1a (n = 20), stx1a + stx2a (n = 8), stx2a (n = 4), stx2b (n = 3), stx2a + stx2c (n = 1), and stx2a + stx2d (n = 1). Polymerase chain reaction screening of other toxin and adherence genes showed that astA, subA, and cdtB were present in 5, 2, and 2 strains, respectively. The intimin gene eae was present in 30 strains (81%). Of the 7 eae-negative strains, saa and eibG were found in 3 and 2 strains, respectively; no adherence factors were detected in the remaining 2 strains. The antimicrobial susceptibility profiles of the strains to 12 drugs were examined and 11 strains (30%) showed resistance to 1 or more drugs. Our results revealed that non-O157/O26 EHEC strains exhibit various biochemical phenotypes and carry several toxins and adherence factor genes.
Microbial Genomics | 2017
Yoshitoshi Ogura; Yasuhiro Gotoh; Takehiko Itoh; Mitsuhiko P. Sato; Kazuko Seto; Shyuji Yoshino; Junko Isobe; Yoshiki Etoh; Mariko Kurogi; Keiko Kimata; Eriko Maeda; D. Piérard; Masahiro Kusumoto; Masato Akiba; Kiyoshi Tominaga; Yumi Kirino; Yuki Kato; Katsuhiko Shirahige; Tadasuke Ooka; Nozomi Ishijima; Ken-ichi Lee; Sunao Iyoda; Jacques Mainil; Tetsuya Hayashi
A key virulence factor of enterohaemorrhagic Escherichia coli (EHEC) is the bacteriophage-encoded Shiga toxin (Stx). Stxs are classified into two types, Stx1 and Stx2, and Stx2-producing strains are thought to cause more severe infections than strains producing only Stx1. Although O26 : H11 is the second most prevalent EHEC following O157 : H7, the majority of O26 : H11 strains produce Stx1 alone. However, Stx2-producing O26 strains have increasingly been detected worldwide. Through a large-scale genome analysis, we present a global phylogenetic overview and evolutionary timescale for E. coli O26 : H11. The origin of O26 has been estimated to be 415 years ago. Sequence type 21C1 (ST21C1), one of the two sublineages of ST21, the most predominant O26 : H11 lineage worldwide, emerged 213 years ago from one of the three ST29 sublineages (ST29C2). The other ST21 lineage (ST21C2) emerged 95 years ago from ST21C1. Increases in population size occurred in the late 20th century for all of the O26 lineages, but most remarkably for ST21C2. Analysis of the distribution of stx2-positive strains revealed the recent and repeated acquisition of the stx2 gene in multiple lineages of O26, both in ST21 and ST29. Other major EHEC virulence genes, such as type III secretion system effector genes and plasmid-encoded virulence genes, were well conserved in ST21 compared to ST29. In addition, more antimicrobial-resistance genes have accumulated in the ST21C1 lineage. Although current attention is focused on several highly virulent ST29 clones that have acquired the stx2 gene, there is also a considerable risk that the ST21 lineage could yield highly virulent clones.
Journal of Veterinary Medical Science | 2015
Mitsuhiro Kameyama; Kiyoshi Tominaga; Junko Yabata; Yasuharu Nomura
Patterns of insertion sequence (IS)629, norV genotype, and Shiga toxin (Stx) genotype distribution were investigated amongst 203 enterohemorrhagic Escherichia coli O157 isolates collected in Yamaguchi Prefecture, Japan, between 2004 and 2013. A total of 114 IS629 patterns were identified; these were divided into eight IS groups (A–H). Ninety isolates carried an intact norV gene, whereas 113 isolates carried a norV with a 204-bp deletion. Other than one isolate from IS group G, all isolates with an intact norV belonged to groups A–F, whereas isolates with a mutant norV belonged to IS groups G and H. Seven stx genotypes were identified, and of those, stx1a/stx2a was predominant (n=105), followed by stx2c (n=32) and stx2a (n=27). The stx1a/stx2a genotype was associated with the mutant norV isolates, whereas isolates with an intact norV had the stx2c genotype. Therefore, certain combinations of IS type and stx genotype appear to be more frequent among O157 clades which may be useful for detection of predominant subtypes in the interest of public health.
Journal of Infection and Chemotherapy | 2015
Mitsuhiro Kameyama; Junko Yabata; Yasuharu Nomura; Kiyoshi Tominaga
In 2013, an outbreak of enterohemorrhagic Escherichia coli (EHEC) O157:H7 occurred in a nursery school in Japan. The outbreak affected 12 school children and five members of their families. All 17 isolates obtained from these individuals were found to be clonal, as determined by pulsed-field gel electrophoresis analysis and multilocus variable number tandem repeat analysis. The antimicrobial susceptibility profiles of the isolates to 20 drugs were examined, with three isolates showing resistance to the extended-spectrum cephalosporins (ESC) and cephamycin, including cefotaxime, ceftazidime, and cefminox. The resistant isolates carried the blaCMY-2 AmpC β-lactamase gene. It is proposed that the ESC-resistant EHEC O157:H7 isolates might have acquired the resistance plasmid encoding the blaCMY-2 gene during human to human infection in the nursery school.
Journal of Veterinary Medical Science | 1993
Tatsuo Ohya; Kiyoshi Tominaga; Muneo Nakazawa
Japanese Journal of Infectious Diseases | 2010
Tadayoshi Ikebe; Yuuko Oguro; Kikuyo Ogata; Chihiro Katsukawa; Junko Isobe; Tomoko Shima; Rieko Suzuki; Hitomi Ohya; Kiyoshi Tominaga; Rumi Okuno; Yumi Uchitani; Yuki Tada; Nobuhiko Okabe; Haruo Watanabe
Journal of Veterinary Medical Science | 2013
Mitsuhiro Kameyama; Takehisa Chuma; Junko Yabata; Kiyoshi Tominaga; Hiroyuki Iwata; Karoku Okamoto
Journal of Veterinary Medical Science | 2012
Mitsuhiro Kameyama; Takehisa Chuma; Tomoki Yokoi; Junko Yabata; Kiyoshi Tominaga; Daisuke Miyasako; Hiroyuki Iwata; Karoku Okamoto
Fems Immunology and Medical Microbiology | 2006
Masatomo Morita; Kadumi Mori; Kiyoshi Tominaga; Jun Terajima; Kenji Hirose; Haruo Watanabe; Hidemasa Izumiya