Kiyoshige Wakabayashi

Orange-I laurate, a new chromogenic substrate for the assay of lipase in blood

Abstract Eight long-chain fatty acid esters (laurates, myristates, palmitates and stearates) of sodium m - or p -(4-hydroxy-1-naphthylazo)-benzenesulfonate were synthesized and assessed as chromogenic substrates for the assay of lipase. All are hydrolyzed in the presence of lipase, the laurates most rapidly. Orange-I laurate (the p -compound) was selected for detailed study. A very selective assay of lipasein the presence of other esterases was achieved by adding sodium dodecylbenzenesulfate and protamine sulfate as inhibitors. Orange-I laurate could be used as a substrate for lipase in serum.