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Featured researches published by Koen Dekker.


Applied Microbiology and Biotechnology | 1992

Efficient production of thermostable Thermus thermophilus xylose isomerase in Escherichia coli and Bacillus brevis

Koen Dekker; Ayumu Sugiura; Hideo Yamagata; Kenji Sakaguchi; Shigezo Udaka

SummaryThe xylose (glucose) isomerase from the thermophile Thermus thermophilus seems to have potential for the development of new isomerization processes using high temperatures and slightly acidic pH. The isomerase has an optimum temperature at 95° C, and is also very stable at high temperatures. The optimum pH is around 7.0, close to where by-product formation is minimal. Since Thermus produces only a little of this useful isomerase, the production of the cloned gene in Escherichia coli and Bacillus brevis were compared. Especially B. brevis was able to produce the isomerase effeciently, more than 1 g/l, in spite of the high G + content (67%) of the Thermus gene, and the presence of codons not frequently used in E. coli or B. brevis.


Archive | 1992

Cloning and Expression of the Xylose (Glucose) Isomerase Gene from the Thermophile Thermus thermophilus HB8

Koen Dekker; Ayumu Sugiura; Hideo Yamagata; Kenji Sakaguchi; Shigezo Udaka

D-xylose isomerase (E.C. 5.3.1.5) is used industrially to catalyze the isomerization of glucose to the sweeter fructose [1]. The isomerization is reversible and the final fructose content depends on the reaction temperature. At present the process temperature is about 60°C, but a higher temperature would give a higher fructose content. Hence there is a continuing interest in new, stable xylose isomerases [2]. Because thermophilic microorganisms are known to produce highly thermostable enzymes, several thermophiles were screened for isomerase activity. Thermus thermophilus HB8, an aerobe thermophile which grows at temperatures up to 75°C, is the most thermophilic organism from which a xylose isomerase gene has been cloned and characterized [3]. The isomerase has a temperature optimum at 95°C and is more thermostable than any other known isomerase.


Journal of Bacteriology | 1991

Xylose (glucose) isomerase gene from the thermophile Thermus thermophilus : cloning, sequencing, and comparison with other thermostable xylose isomerases

Koen Dekker; Hideo Yamagata; K Sakaguchi; Shigezo Udaka


Agricultural and biological chemistry | 1991

Xylose (Glucose) Isomerase Gene from the Thermophile Clostridium thermohydrosulfuricum; Cloning, Sequencing, and Expression in Escherichia coli

Koen Dekker; Hideo Yamagata; Kenji Sakaguchi; Shigezo Udaka


Archive | 1993

Xylose isomerase gene of Thermus aquaticus

Shigezo Udaka; Kenji Sakaguchi; Hideo Yamagata; Koen Dekker


Archive | 1991

Xyloseisomerase gene of thermus aquaticus, xyloseisomerase and process for preparation of fructose

Shigezo Udaka; Kenji Sakaguchi; Hideo Yamagata; Koen Dekker


Agricultural and biological chemistry | 1991

Production of Thermostable Clostridium thermohydrosulfuricum Xylose Isomerase in Bacillus brevis

Koen Dekker; Hideo Yamagata; Kenji Sakaguchi; Shigezo Udaka


Archive | 1991

Xyloseisomerase-Gen von Thermus Aquaticus, Xyloseisomerase und Verfahren zur Herstellung von Fructose Xyloseisomerase gene from Thermus aquaticus xyloseisomerase and methods for producing fructose

Shigezo Udaka; Kenji Sakaguchi; Hideo Yamagata; Koen Dekker


Archive | 1991

Xyloseisomerase-Gen von Thermus Aquaticus, Xyloseisomerase und Verfahren zur Herstellung von Fructose

Shigezo Udaka; Kenji Sakaguchi; Hideo Yamagata; Koen Dekker


Archive | 1991

Xyloseisomerase gene from Thermus aquaticus xyloseisomerase and methods for producing fructose

Shigezo Udaka; Kenji Sakaguchi; Hideo Yamagata; Koen Dekker

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Shigezo Udaka

Tokyo University of Agriculture

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