Koh Nishida

Studies on the Culture Conditions of Higher Plant Cells in Suspension Culture: Part II. Effect of Nutritional Factors on the Growth

To study the influence of cultural conditions on higher plant cells in suspension culture, the effects of nutritional conditions on the growth of suspended cells were investigated. Calluses were induced from 39 species of Nicotiana plants and 6 species of Populus plants on agar slant media, then these were transferred to suspension cultures. Concentrations of 2,4-D and kinetin suitable for incubation of callus from each plant were investigated and species having high growth rates in the appropriate medium were selected.The effects of concentrations of auxins and kinetin, a variety of carbon and nitrogen sources, thiamin and myo-inositol on growth of the selected calluses were also studied. Of these calluses studied, N. glutinosa, N. tabacum var. Xanthi ova and P. hybrids were selected as calluses having high growth rates. Myo-inositol had no effect on any callus growth, and thiamin gave a distinct effect on Populus callus only. Nitrate as a nitrogen and sucrose as a carbon sources, and 2,4-D as an auxin w...

Studies on Storage and Ageing of Leaf Tobacco:Part IX. Changes in the Content of Amino Acid-Sugar Compounds during Ageing

Changes in the content of amino acid-sugar compounds with natural ageing of flue-cured leaf tobaccos were followed during a period of four years. The content of 1-deoxy-1-prolino-fructose, the most prominent component of the amino acid-sugar compounds in the tobacco, increased in the initial period of the storage and then turned to decreasing. Most of the other amino acid-sugar compounds also showed similar changes in content except for glutamine-fructose compound which only decreased throughout the storage period. These changes indicated the possible progress of Maillard type reactions during natural ageing of leaf tobaccos.