Koichi Muramatsu

Serial Evaluation of Human Anterior Cruciate Ligament Grafts by Contrast-Enhanced Magnetic Resonance Imaging: Comparison of Allografts and Autografts

PURPOSE The purpose of this study was to clarify the difference in remodeling between allografts and autografts in anterior cruciate ligament reconstruction. METHODS We examined 24 knees with bone-patellar tendon-bone allografts and 20 knees with central-third bone-patellar tendon-bone autografts. Surgical patients from January 1997 to December 2002 were included in the study, and more than 1 year had passed since surgery. The mean postoperative follow-up was 24.0 months in the allograft group and 21.3 months in the autograft group. Patients with a positive Lachman test were excluded. The operative method and postoperative rehabilitation programs were the same. All allografts were provided by Tokai Regional Bone Bank, the only regional bone bank in Japan. Contrast-enhanced magnetic resonance imaging (1.0 T, T1-weighted, sagittal section, infusion of 0.2-mL/kg gadolinium-diethylenetriamine pentaacetic acid [Gd-DTPA]) was performed for all knees at 1, 4, 6, and 12 months after surgery and performed for some knees irregularly thereafter. The signal/noise quotient (SNQ) of grafts was calculated by use of magnetic resonance imaging based on the method of Weiler et al. (2001) as follows: SNQ = (Signal of ACL graft - Signal of quadriceps tendon)/Signal of background. RESULTS The SNQ of allografts was significantly lower than for autografts 12 months after surgery. As for the SNQ 1 month after surgery, there was no difference before or after Gd-DTPA infusion in the allografts; however, the SNQ was significantly higher after Gd-DTPA infusion in autografts. The SNQ after Gd-DTPA infusion continued to increase until 12 to 24 months in the allograft group but peaked at 4 or 6 months in the autograft group. CONCLUSIONS Compared with autologous tendons, allogeneic tendons have a slower onset and rate of revascularization. LEVEL OF EVIDENCE Level III, retrospective comparative study.

Postoperative magnetic resonance imaging of lumbar disc herniation: comparison of microendoscopic discectomy and Love's method.

Study Design. We performed a study to compare the magnetic resonance imaging findings up to 24 weeks after microendoscopic discectomy or surgery using Love’s method in patients with lumbar disc herniation. Objectives. The objective was to determine whether or not microendoscopic discectomy was minimally invasive with respect to the nerve roots, cauda equina, and paravertebral muscles by comparing the postoperative magnetic resonance imaging findings in patients treated by microendoscopic discectomy and the conventional Love’s method. Summary of Background Data. We introduced microendoscopic discectomy as a minimally invasive surgical procedure for lumbar disc herniation in September 1998 and have obtained good results. Microendoscopic discectomy is superior to the conventional Love’s method in that it reduces postoperative pain, shortens the duration of hospitalization, and allows earlier resumption of normal activities. However, the effect of microendoscopic discectomy on the nerves and paravertebral muscles has not been evaluated objectively. Methods. Enhancement of the nerve roots and paravertebral muscles, as well as the configuration of the cauda equina at the level of herniation, was assessed on axial magnetic resonance images obtained with contrast enhancement using gadolinium-diethylenetriamine penta-acetic acid before surgery and 1, 4, 8, 12, and 24 weeks after surgery in 25 patients who underwent microendoscopic discectomy and 15 patients who were treated using Love’s method. Results. Increased enhancement of the nerve roots was seen in 50.0% of the microendoscopic discectomy group and 46.2% of the Love group at 1 week after surgery. Enhancement of the paravertebral muscles at the surgical site tended to persist for longer in the microendoscopic discectomy group than in the Love group. However, muscle enhancement was widespread in some patients from the Love group. Abnormalities of the cauda equina attributed to surgical invasion were seen in 12.5% of the microscopic discectomy group and 15.4% of the Love group at 1 week after surgery. Conclusions. Microendoscopic discectomy had an effect on the nerve roots and cauda equina that was comparable with that of Love’s method. The magnetic resonance images of the route of entry failed to show that microendoscopic discectomy is appreciably less invasive with respect to the paravertebral muscles.

The effect of heat-treated human bone morphogenetic protein on clinical implantation.

For the clinical usage of human-derived bones, it is necessary to treat bones to reduce the risk of contamination by microorganisms. Bone morphogenetic protein is vulnerable to chemicals, but shows resistance to thermal heat to 70° C in a short time. In this experiment, crude human bone morphogenetic protein was extracted from heat-treated bones at 60° C for 10 hours and from nonheated bones. Sodium dodecyl sulfate polyacrylamide gel electrophoresis for these specimens was done. Gelatin capsules containing 5 mg of crude human bone morphogenetic protein extracted from heated and nonheated bones were implanted into thigh muscle pouches of five mice. At 20 days after implantation, the heterotopic bone formation was compared by evaluating the radiographic and histologic analyses. The sodium dodecyl sulfate polyacrylamide gel electrophoresis pattern of the human bone morphogenetic proteins showed five main bands (16, 22, 28, 35, and 67 kDa) that were almost identical. Heterotopic bone formation observed on the radiograph was induced by crude human bone morphogenetic protein from heated bones in a manner similar to that used for nonheated bones. The results from this study show that heat-treated bone preserves osteoinduction.

3D Variable Refocus Flip Angle-Turbo Spin Echo T2 強調画像を用いた膝anterolateral ligament 描出における三次元画像再構成の有用性

Anterolateral ligament (ALL) is one of the lateral structures in the knee that contributes to the internal rotational stability of tibia. ALL has been referred to in some recent reports to re-emphasize its importance. We visualized the ALL on 3D-MRI in 32 knees of 27 healthy volunteers (23 male knees, 4 female knees; mean age: 37 years). 3D-MRIs were performed using 1.5-T scanner [T(2) weighted image (WI), SPACE: Sampling Perfection with Application optimized Contrast using different flip angle Evolutions] in the knee extended positions. The visualization rate of the ALL, the mean angle to the lateral collateral ligament (LCL), and the width and the thickness of the ALL at the joint level were investigated. The visualization rate was 100%. The mean angle to the LCL was 10.6 degrees. The mean width and the mean thickness of the ALL were 6.4 mm and 1.0 mm, respectively. The ALL is a very thin ligament with a somewhat oblique course between the lateral femoral epicondyle and the mid-third area of lateral tibial condyle. Therefore, the slice thickness and the slice angle can easily affect the ALL visualization. 3D-MRI enables acquiring thin-slice imaging data over a relatively short time, and arbitrary sections aligned with the course of the ALL can later be selected.