Koichiro Saito
Kyorin University
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Publication
Featured researches published by Koichiro Saito.
PLOS ONE | 2015
Yasunao Kogashiwa; Hiroyuki Sakurai; Yoshihiro Akimoto; Dai Sato; Tetsuya Ikeda; Yoshifumi Matsumoto; Yorihisa Moro; Toru Kimura; Yasuhiro Hamanoue; Takehiro Nakamura; Koichi Yamauchi; Koichiro Saito; Masashi Sugasawa; Naoyuki Kohno
Background Sentinel node navigation surgery is gaining popularity in oral cancer. We assessed application of sentinel lymph node navigation surgery to pharyngeal and laryngeal cancers by evaluating the combination of contrast-enhanced ultrasonography and indocyanine green fluorescence in animal models. Methods This was a prospective, nonrandomized, experimental study in rabbit and swine animal models. A mixture of indocyanine green and Sonazoid was used as the tracer. The tracer mixture was injected into the tongue, larynx, or pharynx. The sentinel lymph nodes were identified transcutaneously by infra-red camera and contrast-enhanced ultrasonography. Detection time and extraction time of the sentinel lymph nodes were measured. The safety of the tracer mixture in terms of mucosal reaction was evaluated macroscopically and microscopically. Results Sentinel lymph nodes were detected transcutaneously by contrast-enhanced ultrasonography alone. The number of sentinel lymph nodes detected was one or two. Despite observation of contrast enhancement of Sonazoid for at least 90 minutes, the number of sentinel lymph nodes detected did not change. The average extraction time of sentinel lymph nodes was 4.8 minutes. Indocyanine green fluorescence offered visual information during lymph node biopsy. The safety of the tracer was confirmed by absence of laryngeal edema both macro and microscopically. Conclusions The combination method of indocyanine green fluorescence and contrast-enhanced ultrasonography for detecting sentinel lymph nodes during surgery for head and neck cancer seems promising, especially for pharyngeal and laryngeal cancer. Further clinical studies to confirm this are warranted.
Laryngoscope | 2017
Natsuko Kasakura‐Kimura; Masatsugu Masuda; Hideki Mutai; Sawako Masuda; Noriko Morimoto; Noboru Ogahara; Hayato Misawa; Hirokazu Sakamoto; Koichiro Saito; Tatsuo Matsunaga
Evaluating the prevalence of specific gene mutations associated with a certain audiometric configuration facilitates clinical assessment of patients with sensorineural hearing loss (SNHL). WFS1 is responsible for autosomal dominant nonsyndromic deafness 6/14/38 and is the most frequent genetic cause of low‐frequency SNHL (LFSNHL); however, the exact prevalence of WFS1 mutations in LFSNHL is unknown. Therefore, we evaluated genetic mutations and clinical features in patients with nonsyndromic bilateral LFSNHL, focusing on the WFS1.
Head and Neck-journal for The Sciences and Specialties of The Head and Neck | 2017
Yoshifumi Matsumoto; Hiroyuki Sakurai; Yasunao Kogashiwa; Toru Kimura; Yuma Matsumoto; Takashi Shionome; Masatake Asano; Koichiro Saito; Naoyuki Kohno
Despite improved survival by the addition of a monoclonal antibody against epidermal growth factor receptor (EGFR), cetuximab, to chemotherapy or radiotherapy for squamous cell carcinoma of the head and neck (SCCHN), cetuximab by itself is not a potent antiproliferative agent against SCCHN. We aimed to elucidate working mechanism of cetuximab in SCCHN.
Asia Pacific Allergy | 2017
Hidenori Yokoi; Hiroshi Yoshitake; Yuma Matsumoto; Michitsugu Kawada; Yoshiki Takato; Kiyomi Shinagawa; Hiroyuki Sakurai; Koichiro Saito
Background Specific IgE antibodies against the low-molecular-weight carbohydrate antigen that does not bridge IgE molecules on mast cells are not associated with clinical symptoms. Cross reactivity can be determined in allergen-specific IgE detection assays when the carbohydrate structures between pollen allergens and plant derived food allergens are similar; in such cases, false positive results for grain or legume allergens can be reported for pollen allergic patients who are not sensitized to those allergens. This phenomenon arises owing to the presence of cross-reactive carbohydrate determinants (CCDs). Objective This study aimed to assess the impact of CCD interference on the results for pollen allergen-specific IgE antibodies in the general adult population and to perform CCD inhibition tests evaluating the involvement of CCD on samples positive to pollen allergens. Methods Serum samples from 322 subjects were tested for IgE antibodies to pollens and CCD. The research subjects were given questionnaires about pollen allergic symptoms to help assess the presence of allergies. Allergen IgE antibodies for Japanese cedar, Japanese cypress, orchard grass, ragweed, MUXF, bromelain, horseradish peroxidase (HRP), and ascorbate oxidase (ASOD) were analyzed. Results It was observed that among individuals who tested positive to any of the pollen allergens, the positive ratio of CCD-specific IgE antibody was the highest for HRP (13.5%–50.0%). The results from the inhibition tests revealed that CCD was marginally present. Although IgE antibodies for cedar pollen did not react with CCD, IgE antibodies for Japanese cypress, orchard grass, and ragweed might be detected by the presence of CCD. Conclusion The results of the inhibition tests revealed the obvious presence of CCD suggesting its involvement. Considering these findings, careful evaluation of patient IgE results should be performed for Japanese cypress, orchard grass, and ragweed.
Laryngoscope | 2017
Takeyuki Kono; Koichiro Saito; Haruna Yabe; Kaoru Ogawa
This study was designed to assess the feasibility of the submucosal infusion technique combined with microflap dissection as a radical therapeutic and diagnostic option for precancerous laryngeal leukoplakia.
Cancer Research | 2016
Yoshifumi Matsumoto; Hiroyuki Sakurai; Yasunao Kogashiwa; Koichiro Saito
Background: Recently, irradiation combined with Cetuximab (Cmab) is being one of the standard therapeutic options against head and neck squamous cell carcinoma (HNSCC), especially in advanced/recurrent cases. While this concurrent chemo-radiotherapy is widely accepted and tolerated, Cmab monotherapy has been reported to be unsatisfactory to treat HNSCC, and detailed mechanism of Cmab to show such clinical efficacy is unknown. Methods: To assess the impact of Cmab on proliferation, migration, and invasion of HNSCC, WST assay, wound healing assay, and matrigel invasion assay were incorporated in this study. Effects of Cmab on the grade of epithelial-mesenchymal transition (EMT), expression level of membrane type 1-matrix-metalloproteinase (MT1-MMP), and activity of EGFR-GEP100-Arf6-AMAP1 pathway, were evaluated using the immunocytochemistry, western blot analysis and Arf6 activation assay, respectively. Furthermore, In vivo efficacy of Cmab was evaluated in highly aggressive and metastatic HSC-3-M3 cells using orthotopic xenograft model in nude mice nu/nu. Results: Inhibition of migration and invasion were observed in 4 HNSCC cell lines by Cmab application. However, proliferation potential of HNSCC were not affected by this chemotherapeutic agent. Increased expression of E-cadherin, with decreased expression of N-cadherin and MT1-MMP, were observed after Cmab treatment in vitro to suggest that Cmab may inhibit EMT in HNSCC. Furthermore, inhibition of Tyr 1086 phosphorylation in EGFR, as well as the inactivation of Arf6 coupled with suppression of both GEP100 and AMAP1 expressions were observed by Cmab application. These results suggested that Cmab might have the potential to suppress the activity of EGFR-GEP100-Arf6-AMAP1 pathway. Furthermore, less metastasis of the cancer was observed in the Cmab treatment group compared with control group in vivo. Conclusions: Our study is the first to provide that Cmab could affect HNSCC through inhibition of migration and invasion of cancer cells, regardless of its anti-proliferative action. EMT inhibition after Cmab application was also clarified in this study. Furthermore, our study suggested that EGFR-GEP100-Arf6-AMAP1 pathway may have some role in the invasion and metastasis of HNSCC. Future studies to further assess the precise mechanism of Cmab against HNSCC are warranted. Citation Format: Yoshifumi Matsumoto, Hiroyuki Sakurai, Yasunao Kogashiwa, Koichiro Saito. Cetuximab inhibits migration, invasion, metastasis and epithelial-mesenchymal transition but not proliferation via GEP100-Arf6-AMAP1 pathway in head and neck squamous cell carcinoma. [abstract]. In: Proceedings of the 107th Annual Meeting of the American Association for Cancer Research; 2016 Apr 16-20; New Orleans, LA. Philadelphia (PA): AACR; Cancer Res 2016;76(14 Suppl):Abstract nr 1605.
Radiology Case Reports | 2018
Hiroshi Nagafuji; Hidenori Yokoi; Arisa Ohara; Masachika Fujiwara; Nobuyuki Takayama; Koichiro Saito
Diffuse large B-cell lymphoma arising as a primary tumor in the frontal sinus is very rare. Moreover, it is often difficult to diagnose frontal sinus lesions. A 67-year-old Japanese man initially presented with diplopia and a swollen left upper eyelid. Diffusion-weighted magnetic resonance imaging suggested a malignant lymphoma of the frontal sinus, and subsequent extensive examination revealed diffuse large B-cell lymphoma of the frontal sinus with left orbital invasion. Six courses of combined immunodirected chemotherapy were administered. The patient is tumor-free owing to the accurate diagnosis of lymphoma at an early stage.
Laryngoscope | 2018
Yuma Matsumoto; Hidenori Yokoi; Toru Kimura; Yoshifumi Matsumoto; Michitsugu Kawada; Ken Arae; Susumu Nakae; Tetsuya Ikeda; Kenji Matsumoto; Hiroyuki Sakurai; Koichiro Saito
Gastrin‐releasing peptide (GRP) is a neuropeptide that targets transmembrane‐type receptors. Its role in allergic rhinitis (AR) has yet to be investigated. The present study utilized the nasal mucosa of AR model mice to examine GRP and GRP receptor (GRPR) expression levels, localization, and other factors to evaluate their role in AR pathology.
BMC Clinical Pathology | 2018
Hidenori Yokoi; Yuichi Terado; Masachika Fujiwara; Yuma Matsumoto; Tetsuya Ikeda; Koichiro Saito
BackgroundLow-grade nasopharyngeal papillary adenocarcinoma (LGNPPA) is distinctly rare. We report a patient with a uniquely biphasic LGNPPA; additionally, we review similar tumors reported in the literature.Case presentationA 56-year-old man presented with an asymptomatic pedunculated tumor in the vault of the nasopharynx, at the junction of the nasal septum and the roof, which was discovered during screening for laryngeal cancer. To obtain a definitive diagnosis, the patient underwent endoscopic endonasal surgery under general anesthesia. Immunohistochemical analysis of the tumor revealed it to be an LGNPPA with a prominent spindle cell component.ConclusionTo our knowledge, this is the fourth reported LGNPPA exhibiting a spindle cell component and the second with a prominent pathological condition. The prognosis of LGNPPA is usually excellent. Therefore, it is important for clinicians to scrutinize the lesion’s pathology to avoid unnecessary, disfiguring surgery.
BMC Cardiovascular Disorders | 2018
Satoshi Higuchi; Yusuke Kabeya; Kenichi Matsushita; Keisei Tachibana; Riken Kawachi; Hidefumi Takei; Yutaka Suzuki; Nobutsugu Abe; Yorihisa Imanishi; Kiyoshi Moriyama; Tomoko Yorozu; Koichiro Saito; Masanori Sugiyama; Haruhiko Kondo; Hideaki Yoshino
BackgroundA previous retrospective cohort study established the relationship between perioperative atrial fibrillation (POAF) and subsequent mortality and stroke. However, the details regarding the cause of death and etiology of stroke remain unclear.MethodsThe prospective cohort study of surveillance for perioperative atrial fibrillation recurrence in major non-cardiac surgery for malignancy (PREDICT AF RECURRENCE) registry is an ongoing prospective cohort study to elucidate the long-term recurrence rate and the clinical impact of new-onset POAF in the setting of head and neck, non-cardiac thoracic, and abdominal surgery for malignancy. In this study, cardiologists collaborate with a surgical team during the perioperative period, carefully observe the electrocardiogram (ECG) monitor, and treat arrhythmia as required. Furthermore, patients who develop new-onset POAF are followed up using a long-term Holter ECG monitor, SPIDER FLASH-t AFib®, to assess POAF recurrence.DiscussionEven if patients with malignancy survive by overcoming the disease, they may die from any preventable cardiovascular diseases. In particular, those with POAF may develop cardiogenic stroke in the future. Because details of the natural history of patients with POAF remain unclear, investigating the need to continue anticoagulation therapy for such patients is necessary. This study will provide essential information on the recurrence rate of POAF and new insights into the prediction and treatment of POAF.Trial registrationUniversity Hospital Medical Information Network Clinical Trial Registry (UMIN-CTR): UMIN000016146; Data of Registration: January 7, 2015.