Kosaku Eto

Establishment and characterization of a new human renal cell carcinoma cell line (KRC/Y)

SummaryA new renal cell carcinoma (RCC) cell line (KRC/Y) has been established from a surgical specimen of a 41-yr-old Japanese female patient with RCC composed of both clear cells and granular cells. This cell line has been maintained for more than 15 mo. through 45 passages with a stable growth, KRC/Y cells have clear or eosinophilic polygonal cytoplasm and round to oval nuclei with one or two nucleoli, and proliferate in a pavementlike cell arrangement with a lack of contanct inhibition. By electron microscopy, these cells contain abundant fat droplets and glycogen granules or well-developed organells or both, which were also observed in the original tumor. The doubling time of these cells at the 15th passage was 73 h. The chromosome number was from 37 to 45 with a hypodiploid modal number of 42. Tumorigenicity was identified by tumor formation after subcutaneous injections of KRC/Y cells in nude mice, which showed close resemblance to the original tumor by light and electron microscope observations.

Treatment of Complicated Urinary Tract Infections with Ofloxacin following an Aminoglycoside

In order to reduce the dosage of aminoglycoside in the treatment of patients with complicated urinary tract infections (UTI) in outpatient clinics and to improve cost benefits, we tried both ofloxacin (OFX) treatment and a combination treatment with OFX and a single dose of aminoglycoside (isepamicin). Both groups showed the same cure rate on day 5, but the bacteriuria elimination rate on day 1 was slightly higher in the isepamicin-OFX group. The recurrence rate on days 7-14 did not differ between the groups. This aminoglycoside-OFX treatment did not show significantly higher effectiveness against complicated UTI but resulted in earlier elimination of bacteriuria. These results suggest that this combination therapy may be cost effective in treating complicated UTI, because of the shorter treatment period required.

Studies of urodynamics in hemodialysis patients

血液透析患者の排尿機能に関する報告は今まであまりなされていない. 今回uroflowmetry (UFM) を施行して, その尿流量曲線より尿流量率を解析し, 透析期間, 尿路感染症 (UTI) の有無, 透析レベル, 尿量, 末梢神経伝導速度などとの相関性を検討した. その結果, 1) 細菌尿は23例中10例 (43.5%) に検出され, 4例 (17.4%) がUTIであった. 細菌尿陽性は透析期間と有意の正の相関を示した. 2) 細菌尿と1日尿量との間には負の相関を認めた. また, 残尿量は予想に反し全例で無視できる量であった. 3) 神経伝導速度は13例中12例 (92.3%) が正常で, UFMの結果とは全く関係はなかった. 4) UFM判定では23例中9例 (39.1%) が排尿異常を示した. 5) 排尿異常は透析期間との間に負の相関を認めた.以上より, 透析が長期間になるにしたがって膀胱機能低下が起こってくる可能性があることが示された. また末梢神経障害による排尿機能障害は否定的な結果が得られたが, 原因については今後の詳細な検討を要する. UTIとの関係については, 比較的長期の透析患者では, 尿流量率の低下のみが起こっており, このことがUTI発症に何らかの影響を与えているのではないかと考えられた.