Kostas D. Zarpas

Morphological discrimination of Aphis gossypii (Hemiptera: Aphididae) populations feeding on Compositae

Aphis gossypii Glover is a polyphagous aphid pest with a worldwide distribution. However, there is evidence that on a global scale the name A. gossypii is being applied to a number of forms with different life cycles and/or host-plant associations. Morphometric variation of A. gossypii samples from crops and non-cultivated plants in many parts of the world was examined, to determine whether this variation is correlated with the hosts from which the aphids originated. Samples of A. gossypii were collected from Cucurbitaceae and Malvaceae in Europe, and from Compositae in various parts of the world. Morphometric data for 13 parameters measured from 97 clonal lineages (728 specimens) and 27 field-collected samples (313 specimens) were analysed by a series of canonical variates analyses, using the field sample/clonal lineage as grouping factor. Clonal lineages were reared on a common host in controlled conditions to standardize the effect of host and environment on morphology. The analyses provided a clear morphometric separation of the aphids originating from Compositae and those collected on Cucurbitaceae and Malvaceae, regardless of the geographical origin of the aphids and the host plant on which they were reared. This indicates that within A. gossypii there are two widely distributed host races or subspecies with different plant family associations. The taxonomic implications are discussed.

Performance of cotton aphid Aphis gossypii (Hemiptera: Aphididae) lineages on cotton varieties

Abstract The performance of three clonal lineages of Aphis gossypii Glover originating from central Greece was studied on six commercial cotton cultivars that differ with respect to leaf pubescence traits (Zeta-2, Zeta-5, Eva, Korina, Acala-SJ2 and Sindos-80). Two main laboratory experiments were conducted: (i) one clonal lineage was studied on all six varieties; and (ii) four varieties were assayed with two other lineages. Aphid development, fecundity, longevity and mortality were recorded and life-table characteristics estimated for all varieties. The results showed that leaf trichome density was significantly correlated with aphid performance (i.e. positive correlation with intrinsic rate of increase and fecundity, and negative correlation with developmental time) in both experiments. Leaf hairiness was at least partly responsible for the poorer performance in cotton aphid lineages. We hypothesise that other factors such as allelochemicals (e.g., gossypol, tannins), which are known to affect hemipteran insects, also might be involved.

Toxic and hormetic-like effects of three components of citrus essential oils on adult Mediterranean fruit flies (Ceratitis capitata)

Plant essential oils (EOs) and a wide range of their individual components are involved in a variety of biological interactions with insect pests including stimulatory, deterrent, toxic and even hormetic effects. Both the beneficial and toxic properties of citrus EOs on the Mediterranean fruit fly (medfly) have been experimentally evidenced over the last years. However, no information is available regarding the toxic or beneficial effects of the major components of citrus EOs via contact with the adults of the Mediterranean fruit fly. In the present study, we explored the toxicity of limonene, linalool and α-pinene (3 of the main compounds of citrus EOs) against adult medflies and identified the effects of sub-lethal doses of limonene on fitness traits in a relaxed [full diet (yeast and sugar)] and in a stressful (sugar only) feeding environment. Our results demonstrate that all three compounds inferred high toxicity to adult medflies regardless of the diet, with males being more sensitive than females. Sub-lethal doses of limonene (LD20) enhanced the lifespan of adult medflies when they were deprived of protein. Fecundity was positively affected when females were exposed to limonene sub-lethal doses. Therefore, limonene, a major constituent of citrus EOs, induces high mortality at increased doses and positive effects on life history traits of medfly adults through contact at low sub-lethal doses. A hormetic-like effect of limonene to adult medflies and its possible underlying mechanisms are discussed.